LOCUS BBa_S04308 3053 bp DNA linear 19-Apr-2024 DEFINITION S04306:S04307 ACCESSION BBa_S04308 VERSION BBa_S04308.1 FEATURES Location/Qualifiers misc_feature 9..25 /label=OR1 misc_feature 33..49 /label=OR2 promoter 48..53 /label=-35 misc_feature 59..65 /label=mutate to obliterate OR3 promoter 71..76 /label=-10 promoter 1..82 /label=BBa_I12007 misc_feature 95..98 /label=conserved RBS 91..102 /label=BBa_B0034 misc_feature 688..719 /label=LVA CDS 109..687 /label=luxI misc_feature 109..726 /label=BBa_C0061 misc_feature 727..751 /label=Help:Barcodes CDS 109..726 /label=BBa_C0061 stem_loop 771..814 /label=stem_loop misc_feature 760..839 /label=BBa_B0010 terminator 760..839 /label=BBa_B0010 stem_loop 855..874 /label=T7 TE misc_feature 881..881 /label=stop polyA_site 875..888 /label=polya misc_feature 848..888 /label=BBa_B0012 terminator 848..888 /label=BBa_B0012 misc_feature 91..888 /label=BBa_F1610 misc_feature 1..888 /label=BBa_S04306 promoter 897..1026 /label=BBa_I13453 misc_feature 1039..1042 /label=conserved RBS 1035..1046 /label=BBa_B0034 misc_feature 2142..2180 /label=LVA CDS 1053..2180 /label=lacI-LVA misc_feature 1053..2180 /label=BBa_C0012 misc_feature 2181..2205 /label=Help:Barcodes CDS 1053..2180 /label=BBa_C0012 misc_feature 1035..2205 /label=BBa_S0100 promoter 897..2205 /label=BBa_K091225 misc_feature 2218..2221 /label=conserved RBS 2214..2225 /label=BBa_B0034 CDS 2235..2851 /label=tetR misc_feature 2852..2885 /label=SsrA misc_feature 2892..2916 /label=Help:Barcodes CDS 2232..2891 /label=BBa_C0040 stem_loop 2936..2979 /label=stem_loop misc_feature 2925..3004 /label=BBa_B0010 terminator 2925..3004 /label=BBa_B0010 stem_loop 3020..3039 /label=T7 TE misc_feature 3046..3046 /label=stop polyA_site 3040..3053 /label=polya misc_feature 3013..3053 /label=BBa_B0012 terminator 3013..3053 /label=BBa_B0012 misc_feature 2214..3053 /label=BBa_P0440 misc_feature 897..3053 /label=BBa_S04307 ORIGIN 1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa 61 atagtggtga tagatttaac gttactagag aaagaggaga aatactagat gactataatg 121 ataaaaaaat cggatttttt ggcaattcca tcggaggagt ataaaggtat tctaagtctt 181 cgttatcaag tgtttaagca aagacttgag tgggacttag ttgtagaaaa taaccttgaa 241 tcagatgagt atgataactc aaatgcagaa tatatttatg cttgtgatga tactgaaaat 301 gtaagtggat gctggcgttt attacctaca acaggtgatt atatgctgaa aagtgttttt 361 cctgaattgc ttggtcaaca gagtgctccc aaagatccta atatagtcga attaagtcgt 421 tttgctgtag gtaaaaatag ctcaaagata aataactctg ctagtgaaat tacaatgaaa 481 ctatttgaag ctatatataa acacgctgtt agtcaaggta ttacagaata tgtaacagta 541 acatcaacag caatagagcg atttttaaag cgtattaaag ttccttgtca tcgtattgga 601 gacaaagaaa ttcatgtatt aggtgatact aaatcggttg tattgtctat gcctattaat 661 gaacagttta aaaaagcagt cttaaatgct gcaaacgacg aaaactacgc tttagtagct 721 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa 781 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 841 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacat 901 tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc 961 ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc gtttttttgg 1021 gctagctact agagaaagag gagaaatact agatggtgaa tgtgaaacca gtaacgttat 1081 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg 1141 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt 1201 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg 1261 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg 1321 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct 1381 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc 1441 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat 1501 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta 1561 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg 1621 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc 1681 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc 1741 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg 1801 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg 1861 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa 1921 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac 1981 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa 2041 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 2101 tgcagctggc acgacaggtt tcccgactgg aaagcgggca ggctgcaaac gacgaaaact 2161 acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta gagaaagagg 2221 agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc 2281 ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag 2341 agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca 2401 ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa agctggcaag 2461 attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag 2521 caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat 2581 tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg 2641 tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag 2701 aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct atcgaattat 2761 ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat 2821 tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag 2881 tagcttaata acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa 2941 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 3001 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //