##gff-version 3
##sequence-region BBa_S04308 1 3053
BBa_S04308	.	engineered_region	1	888	.	+	0	ID=BBa_S04306;Name=BBa_S04306
BBa_S04308	.	engineered_region	91	888	.	+	0	ID=BBa_F1610;Name=BBa_F1610;Parent=BBa_S04306
BBa_S04308	.	terminator	848	888	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_S04308	.	polyA_site	875	888	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04308	.	stem_loop	855	874	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04308	.	engineered_region	848	888	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04308	.	stop_codon	881	881	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04308	.	terminator	760	839	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_S04308	.	stem_loop	771	814	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04308	.	engineered_region	760	839	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04308	.	ribosome_entry_site	91	102	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_S04308	.	conserved_region	95	98	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04308	.	CDS	109	726	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_S04308	.	CDS	109	687	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S04308	.	engineered_tag	727	751	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S04308	.	engineered_region	109	726	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S04308	.	tag	688	719	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S04308	.	promoter	1	82	.	+	0	ID=BBa_I12007;Name=BBa_I12007;Parent=BBa_S04306
BBa_S04308	.	non_covalent_binding_site	33	49	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_S04308	.	promoter	48	53	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_S04308	.	promoter	71	76	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_S04308	.	sequence_alteration	59	65	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_S04308	.	non_covalent_binding_site	9	25	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_S04308	.	engineered_region	897	3053	.	+	0	ID=BBa_S04307;Name=BBa_S04307
BBa_S04308	.	engineered_region	2214	3053	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_S04307
BBa_S04308	.	terminator	2925	3004	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_S04308	.	stem_loop	2936	2979	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04308	.	engineered_region	2925	3004	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04308	.	ribosome_entry_site	2214	2225	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_S04308	.	conserved_region	2218	2221	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04308	.	terminator	3013	3053	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_S04308	.	polyA_site	3040	3053	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04308	.	stem_loop	3020	3039	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04308	.	engineered_region	3013	3053	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04308	.	stop_codon	3046	3046	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04308	.	CDS	2232	2891	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_S04308	.	engineered_tag	2892	2916	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S04308	.	CDS	2235	2851	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S04308	.	tag	2852	2885	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S04308	.	promoter	897	2205	.	+	0	ID=BBa_K091225;Name=BBa_K091225;Parent=BBa_S04307
BBa_S04308	.	promoter	897	1026	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091225
BBa_S04308	.	engineered_region	1035	2205	.	+	0	ID=BBa_S0100;Name=BBa_S0100;Parent=BBa_K091225
BBa_S04308	.	CDS	1053	2180	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_S04308	.	engineered_region	1053	2180	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S04308	.	CDS	1053	2180	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S04308	.	engineered_tag	2181	2205	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S04308	.	tag	2142	2180	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S04308	.	ribosome_entry_site	1035	1046	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_S04308	.	conserved_region	1039	1042	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S04308
gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaa
cgttactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggagga
gtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataacctt
gaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggc
gtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaaga
tcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattaca
atgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaa
tagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaa
atcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgct
ttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatc
cataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagct
actagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtc
tcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcga
tggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccac
ctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtg
gtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtg
ggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatt
tcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcat
ctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctg
gctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttt
tcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggc
gcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagaca
gctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgct
gcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcg
cccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaa
gcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactaga
gaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtc
ggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaa
ataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagggga
aagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagta
catttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggttttt
cactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatca
agtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgat
caccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaa
gtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata