##gff-version 3
##sequence-region BBa_S04325 1 6114
BBa_S04325	.	terminator	3834	3913	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S04325	.	stem_loop	3845	3888	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04325	.	engineered_region	3834	3913	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04325	.	engineered_region	1	3825	.	+	0	ID=BBa_K218000;Name=BBa_K218000
BBa_S04325	.	point_mutation	599	599	.	+	0	ID=annotation2048335;Name=Silent mutation to remove EcoRI site;Parent=BBa_K218000
BBa_S04325	.	CDS	1127	3706	.	+	0	ID=annotation2048322;Name=LuxQ;Parent=BBa_K218000
BBa_S04325	.	stop_codon	1125	1127	.	+	0	ID=annotation2048319;Name=stop for LuxP;Parent=BBa_K218000
BBa_S04325	.	start_codon	1127	1129	.	+	0	ID=annotation2048299;Name=start of LuxQ;Parent=BBa_K218000
BBa_S04325	.	start_codon	30	32	.	+	0	ID=annotation2048298;Name=start of LuxP;Parent=BBa_K218000
BBa_S04325	.	stop_codon	3704	3706	.	+	0	ID=annotation2048321;Name=stop for LuxQ;Parent=BBa_K218000
BBa_S04325	.	point_mutation	1618	1618	.	+	0	ID=annotation2048337;Name=Silent mutation to remove XbaI site;Parent=BBa_K218000
BBa_S04325	.	CDS	30	1127	.	+	0	ID=annotation2048320;Name=LuxP;Parent=BBa_K218000
BBa_S04325	.	point_mutation	1465	1465	.	+	0	ID=annotation2048336;Name=Silent mutation to remove EcoRI site;Parent=BBa_K218000
BBa_S04325	.	terminator	3922	3962	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S04325	.	stem_loop	3929	3948	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04325	.	polyA_site	3949	3962	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04325	.	stop_codon	3955	3955	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04325	.	engineered_region	3922	3962	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04325	.	terminator	5986	6065	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S04325	.	stem_loop	5997	6040	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04325	.	engineered_region	5986	6065	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04325	.	terminator	6074	6114	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S04325	.	stem_loop	6081	6100	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04325	.	polyA_site	6101	6114	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04325	.	stop_codon	6107	6107	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04325	.	engineered_region	6074	6114	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04325	.	engineered_region	4033	5977	.	+	0	ID=BBa_K218001;Name=BBa_K218001
BBa_S04325	.	ribosome_entry_site	4037	4044	.	+	0	ID=annotation2048769;Name=RBS;Parent=BBa_K218001
BBa_S04325	.	start_codon	4063	4065	.	+	0	ID=annotation2048770;Name=Start of LuxO;Parent=BBa_K218001
BBa_S04325	.	CDS	5421	5765	.	+	0	ID=annotation2048776;Name=LuxU;Parent=BBa_K218001
BBa_S04325	.	stop_codon	5422	5424	.	+	0	ID=annotation2048771;Name=Stop for LuxO;Parent=BBa_K218001
BBa_S04325	.	stop_codon	5763	5765	.	+	0	ID=annotation2048775;Name=Stop for LuxU;Parent=BBa_K218001
BBa_S04325	.	start_codon	5421	5423	.	+	0	ID=annotation2048774;Name=Start of LuxU;Parent=BBa_K218001
BBa_S04325	.	CDS	4063	5424	.	+	0	ID=annotation2048773;Name=LuxO;Parent=BBa_K218001
BBa_S04325	.	promoter	3971	4024	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S04325	.	engineered_region	3971	4024	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04325	.	promoter	3990	3995	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04325	.	non_covalent_binding_site	3996	4014	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04325	.	promoter	4013	4018	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S04325	.	non_covalent_binding_site	3971	3989	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_S04325
atgctcgataaaaactaaaagagcaatagatgaagaaagcgttactattttccctgatttctatggtcggtttttctcc
agcgtctcaagcaacacaagttttgaatgggtactggggttatcaagagtttttggacgagtttcccgagcaacgaaat
ctgaccaatgctttatcagaagcagtacgagcacagccggtcccactgtctaaaccgacacaacgcccgattaaaatat
cagtggtttacccaggacagcaagtttcagattactgggtaagaaatattgcatcattcgaaaaacgtttgtataagtt
gaatattaactaccaactgaaccaagtgtttactcgtccaaatgctgatatcaagcaacaaagcttgtcattaatggaa
gcgctcaagagcaaatcggattacttgattttcacgcttgatacgacaagacaccgtaaatttgttgagcacgttttgg
actcaacgaacaccaaattgatcttgcaaaatatcactacaccagtccgtgagtgggacaaacatcaaccgtttttata
tgtcggatttgaccacgcagaaggcagtcgtgaattagcaacagagttcggaaagttcttcccaaaacacacatattac
agtgtgctctacttttctgaaggttatattagcgatgtgagaggtgatacttttattcaccaagtaaaccgtgataata
actttgagctacaatcagcgtattacacgaaggcaaccaagcaatccggctatgatgctgcgaaagcgagtttagcaaa
acatccagatgttgattttatctatgcatgttcgaccgacgtagcattaggtgcagtagacgcactggctgagttggga
cgtgaagatattatgatcaatggctggggtggaggctctgctgagttagacgctatccagaagggtgatttagacatca
ccgtcatgcgtatgaatgatgacactggcatagccatggcagaagcgattaagtgggacttggaagataaaccagttcc
gaccgtatactcaggtgactttgaaatcgtaacaaaggcagattcaccggagagaatcgaagcgctgaaaaagcgcgca
tttagatattcagataattgatgacaacaacgcgatcaaacattaaaaagcgtcgctcgctggcgacgctcataacaaa
gatcatcattttagttcttgccccaattattctggggattttcattcagagctattacttctccaagcaaatcatttgg
caagaagtagaccgaaccaaacagcaaacctctgcactgatccacaatatatttgatagccactttgcggcgatccaga
tacatcatgacagtaattccaagagcgaagtcattcgtgatttctacactgatcgcgacacggatgtgctcaacttttt
cttcctcagtatcgaccaaagcgatccgtcgcacacaccagagttccgttttctaacggaccacaaaggcatcatttgg
gacgatggaaatgcgcatttctatggtgtgaacgaccttatccttgatagccttgccaatcgggtcagtttcagtaaca
actggtattacattaatgtcatgacctccattggttcgagacacatgctcgtgcgccgtgtgccgatcctagacccttc
aacaggagaggtgcttggtttctcatttaatgccgtcgtcttagacaacaacttcgctttgatggaaaagctcaagagt
gaaagtaacgtcgacaatgtggtgctggttgctaatagcgttcctttagcaaactctttgattggtgatgagccatata
acgttgctgatgtattgcagcgtaaaagttcagacaaaagactcgataagctgttggtaatagaaacgccaatcgtcgt
aaatgcagtgactaccgagctttgcttgttgacggtacaagacaatcagagtgtggtgacattacaaatccaacatatt
ctagccatgcttgcatcgatcatcggtatgatcatgattgccttaatgagtagggaatggattgagagtaaagtttcgg
cgcagttagaatctttgatgtcttacacccgctctgctcgtgaggaaaaagggtttgaacgatttggcggttcggatat
tgaagagtttgatcacatcggttcaacccttgaaagtacattcgaagagcttgaagcgcagaagaagtcgttccgagat
ctgtttaattttgccttatcacccatcatggtttggtctgaagagagtgtcctgattcagatgaaccctgccgcgcgca
aagaattagtgatcgaagacgatcatgaaatcatgcatccggtcttccaaggctttaaagagaaattgaccccacacct
caaaatggcggctcaaggtgcgacgttgactggggtgaacgtgcctattggtaataagatctaccgatggaacttgtcg
ccaattcgtgttgatggcgatatcagtggcattattgtgcaaggccaagacattacaacacttatcgaagccgagaagc
agagtaacattgcgcgtagagaagcagaaaaatcggcgcaagcacgtgcagacttccttgctaaaatgagccatgaaat
tcgtacgccaatcaacggcattttaggtgtcgcccaattattgaaagattctgtcgatacacaagagcagaagaatcaa
atcgacgtcctgtgccacagtggcgagcacttgcttgcagtactgaacgatattctcgatttctcaaagatagagcagg
gcaagttcaatattcagaaacacccgttctccttcaccgataccatgcgtacattggaaaatatttatcgtccgatttg
cacaaataagggggtggagttggtcatcgagaatgagcttgacccgaatgttgaaatcttcaccgatcaagtccgcttg
aatcagattctatttaacttagtgagtaatgccgttaagttcacgccgattggctcgattcgactgcacgcagaacttg
aacaattctatggtgcggagaacagcgtgttagttgtggaactgactgatactggcatcggcattgaaagcgataagct
cgaccaaatgttcgaaccttttgtgcaagaagagtcgacaaccacacgcgaatatggcggtagcggcctaggtttgacc
atcgttaagaacctagtcgatatgttagaaggtgatgttcaggtccgcagtagcaaggggggggggacaacatttgtta
taacacttccagtaaaagatcgtgagcgtgtcttaaggcctctggaggtcagtcaacgtatcaagccggaagccttgtt
tgatgaaagtttaaaagtgctactggtggaagataaccataccaatgcgtttatccttcaggctttctgtaagaagtat
aaaatgcaggtggattgggcgaaagatgggctggacgcgatggagctcctttctgataccacctacgatctgatcctca
tggataaccaattaccccaccttggtggtattgagaccacgcacgagattcgccagaacttgaggcttggaacgccaat
ttacgcgtgtacagcagacaccgcgaaagaaaccagtgatgcgtttatggcggcaggtgcaaactatgtcatgctgaag
ccaattaaagagaatgcgttacatgaggcgtttgtcgatttcaaacaacgtttcttggtagaaagaacctaacggttta
atggcagtgataagttaggggctgaagttaaataataaaagtaaagaagggagcgtaatgcactaatgccgttcactta
aggtgatcggcattgttttttctagggtatcggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactacta
gagacagaaggtcaaaagtctcgttatctacttatggtagaagacaccgcatccgttgcggcactttaccgctcttacc
tcacgccacttggcatcgatatcaatattgttggaacaggcagagacgccattgaaagcctgaaccatcgcattcctga
tcttattctgctcgatcttcgtctacctgatatgacggggatggacgtattgcacgcggtgaagaaaagccacccagac
gtgccaatcatcttcatgacagcccatggttctatcgatactgcggtagaggcgatgcgccacggttctcaagacttcc
taatcaaaccatgtgaagcagaccgtttacgtgtcacggtgaacaatgcgatccgtaaagcaaccaaattaaagaatga
agctgacaaccccggtaaccaaaattaccaaggcttcatcggcagtagccaaacgatgcagcaggtttaccgcaccatt
gactcggcagcgagcagtaaagcgagtattttcatcacgggtgaaagtggtacgggtaaagaagtgtgtgccgaagcga
ttcacgcagcaagcaaacgcggtgataagccgtttatcgccatcaactgtgcggcaatcccgaaagaccttattgaaag
tgagctgtttggtcacgtaaaaggtgcgtttactggtgctgcgaatgaccgacaaggtgcggcagagcttgctgatggc
ggcaccttgttccttgatgaactctgtgaaatggacttggatcttcaaactaagctattgcgctttatccaaacgggta
cattccaaaaagtcggttcttctaaaatgaagagcgtggatgtgcgctttgtgtgtgcaactaaccgagacccttggaa
agaagtgcaagaaggccgtttccgtgaagacttgtattaccgtttgtacgtgattcctttgcaccttccgccgctgcgt
gagcgtggtaaagacgttattgaaattgcatactcgttgcttggttatatgtctcatgaggaaggtaagagtttcgtcc
gtttcgcacaagacgtgattgaaagattcaacagctacgaatggccgggtaacgttcgccagttgcaaaacgtattgcg
taatatcgtggtactgaacaatggcaaagagatcacgctggatatgttaccgccaccactgaatcagcctgttgtgcgc
caatcggtagcaaaattcattgaacctgacattatgacggtgtcagatattatgccgctttggatgacagagaaaatgg
ctattgagcaggcaattcaagcgtgtgaaggcaacattccacgcgctgctggctatttggatgttagtccatcaacgat
ttatcgcaagttgcaagcttggaatagcaaggacgaaaaacaaaacgtatgaatacggacgtattaaatcagcaaaaaa
ttgaagaactgtctgcggaaattggtagcgataatgttcctgttttgcttgatatttttcttggggaaatggactccta
cattggcactttaactgaacttcagggctcagagcagctgttgtatttaaaagagatcagccacgcactgaaaagtagt
gctgccagctttggcgcagatcgattgtgtgaacgagcgattgccatcgacaagaaagcaaaagcgaatcaattgcaag
agcaggggatggagacgagcgaaatgctcgctttacttcatatcactcgtgacgcctaccgttcttggacaaactaacg
tttcgagcaagacattaagcgcaacgtaaaaacacaaagcccttccggtgtggaagggcttttttgtttggggagtttg
ctccgaatcgaagccgctttctcaatgcttttcgtctagttagacagtaagcgctccataaaacccgcattctaatcgc
ctagcgcgaagaataagatcaagtctccaaccatgaggagatttgaaatgggtactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata