LOCUS       BBa_S04332               892 bp    DNA     linear       23-Dec-2024
DEFINITION  R0085:I13507
ACCESSION   BBa_S04332
VERSION     BBa_S04332.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     CDS             50..724
                     /label=mrfp1
     misc_feature    731..755
                     /label=Help:Barcodes
     CDS             50..730
                     /label=BBa_E1010
     stem_loop       775..818
                     /label=stem_loop
     misc_feature    764..843
                     /label=BBa_B0010
     terminator      764..843
                     /label=BBa_B0010
     stem_loop       859..878
                     /label=T7 TE
     misc_feature    885..885
                     /label=stop
     polyA_site      879..892
                     /label=polya
     misc_feature    852..892
                     /label=BBa_B0012
     terminator      852..892
                     /label=BBa_B0012
     misc_feature    32..892
                     /label=BBa_I13507
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc
       61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      481 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
      541 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
      601 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
      661 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
      721 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa
      781 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      841 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//