LOCUS       BBa_S04346              2801 bp    DNA     linear       22-Nov-2024
DEFINITION  S04344:J31006
ACCESSION   BBa_S04346
VERSION     BBa_S04346.1  
FEATURES             Location/Qualifiers
     misc_feature    86..91
                     /label=-35 Region
     misc_feature    75..93
                     /label=LasR Binding Site
     misc_feature    113..118
                     /label= -10 Region
     misc_feature    125..125
                     /label=Primary Transcriptional Start
     promoter        1..126
                     /label=BBa_K091117
     misc_feature    139..142
                     /label=conserved
     RBS             135..146
                     /label=BBa_B0034
     misc_feature    1..146
                     /label=BBa_S04340
     misc_feature    153..154
                     /label=prefix
     misc_feature    326..326
                     /label=T
     misc_feature    644..644
                     /label=A
     CDS             153..902
                     /label=luxR
     misc_feature    153..908
                     /label=BBa_C0062
     misc_feature    909..933
                     /label=Help:Barcodes
     CDS             153..908
                     /label=BBa_C0062
     misc_feature    1..933
                     /label=BBa_S04341
     misc_feature    946..949
                     /label=conserved
     RBS             942..953
                     /label=BBa_B0034
     misc_feature    1..953
                     /label=BBa_S04343
     misc_feature    1539..1570
                     /label=LVA
     CDS             960..1538
                     /label=luxI
     misc_feature    960..1577
                     /label=BBa_C0061
     misc_feature    1578..1602
                     /label=Help:Barcodes
     CDS             960..1577
                     /label=BBa_C0061
     misc_feature    1..1602
                     /label=BBa_S04344
     CDS             1611..2801
                     /label=tetA(C) backwards
     misc_feature    1611..1613
                     /label=stop
     primer_bind     1611..1630
                     /label=Rev primer J31006
     primer_bind     2778..2801
                     /label=Fwd primer J31006
     CDS             1611..2801
                     /label=BBa_J31006
ORIGIN
        1 tgttctcgtg tgaagccatt gctctgatct tttggacgtt tcttcgagcc tagcaagggt
       61 ccgggttcac cgaaatctat ctcatttgct agttataaaa ttatgaaatt tgtataaatt
      121 cttcagtact agagaaagag gagaaatact agatgaaaaa cataaatgcc gacgacacat
      181 acagaataat taataaaatt aaagcttgta gaagcaataa tgatattaat caatgcttat
      241 ctgatatgac taaaatggta cattgtgaat attatttact cgcgatcatt tatcctcatt
      301 ctatggttaa atctgatatt tcaatcctag ataattaccc taaaaaatgg aggcaatatt
      361 atgatgacgc taatttaata aaatatgatc ctatagtaga ttattctaac tccaatcatt
      421 caccaattaa ttggaatata tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa
      481 ttaaagaagc gaaaacatca ggtcttatca ctgggtttag tttccctatt catacggcta
      541 acaatggctt cggaatgctt agttttgcac attcagaaaa agacaactat atagatagtt
      601 tatttttaca tgcgtgtatg aacataccat taattgttcc ttctctagtt gataattatc
      661 gaaaaataaa tatagcaaat aataaatcaa acaacgattt aaccaaaaga gaaaaagaat
      721 gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata ttaggttgca
      781 gtgagcgtac tgtcactttc catttaacca atgcgcaaat gaaactcaat acaacaaacc
      841 gctgccaaag tatttctaaa gcaattttaa caggagcaat tgattgccca tactttaaaa
      901 attaataaca ctgatagtgc tagtgtagat cactactaga gaaagaggag aaatactaga
      961 tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag tataaaggta
     1021 ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta gttgtagaaa
     1081 ataaccttga atcagatgag tatgataact caaatgcaga atatatttat gcttgtgatg
     1141 atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat tatatgctga
     1201 aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct aatatagtcg
     1261 aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct gctagtgaaa
     1321 ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt attacagaat
     1381 atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa gttccttgtc
     1441 atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt gtattgtcta
     1501 tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg
     1561 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag ttaggtcgag
     1621 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
     1681 cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg
     1741 atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt
     1801 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     1861 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
     1921 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
     1981 aaacgtttgg tggcggggcc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
     2041 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
     2101 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
     2161 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     2221 aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
     2281 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
     2341 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     2401 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     2461 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat ccacaggacg
     2521 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
     2581 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
     2641 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
     2701 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
     2761 acggtgccga ggatgacgat gagcgcattg ttagatttca t
//