##gff-version 3
##sequence-region BBa_S04349 1 2841
BBa_S04349	.	engineered_region	1	2821	.	+	0	ID=BBa_S04348;Name=BBa_S04348
BBa_S04349	.	CDS	1631	2821	.	+	0	ID=BBa_J31006;Name=BBa_J31006;Parent=BBa_S04348
BBa_S04349	.	CDS	1631	2821	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S04349	.	primer_binding_site	1631	1650	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_S04349	.	stop_codon	1631	1633	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S04349	.	primer_binding_site	2798	2821	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_S04349	.	engineered_region	1	1622	.	+	0	ID=BBa_S04345;Name=BBa_S04345;Parent=BBa_S04348
BBa_S04349	.	ribosome_entry_site	1611	1622	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04345
BBa_S04349	.	conserved_region	1615	1618	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04349	.	engineered_region	1	1602	.	+	0	ID=BBa_S04344;Name=BBa_S04344;Parent=BBa_S04345
BBa_S04349	.	engineered_region	1	953	.	+	0	ID=BBa_S04343;Name=BBa_S04343;Parent=BBa_S04344
BBa_S04349	.	engineered_region	1	933	.	+	0	ID=BBa_S04341;Name=BBa_S04341;Parent=BBa_S04343
BBa_S04349	.	engineered_region	1	146	.	+	0	ID=BBa_S04340;Name=BBa_S04340;Parent=BBa_S04341
BBa_S04349	.	promoter	1	126	.	+	0	ID=BBa_K091117;Name=BBa_K091117;Parent=BBa_S04340
BBa_S04349	.	start_codon	125	125	.	+	0	ID=annotation1964075;Name=Primary Transcriptional Start;Parent=BBa_K091117
BBa_S04349	.	sequence_feature	86	91	.	+	0	ID=annotation1964072;Name=-35 Region;Parent=BBa_K091117
BBa_S04349	.	sequence_feature	113	118	.	+	0	ID=annotation1964074;Name= -10 Region;Parent=BBa_K091117
BBa_S04349	.	non_covalent_binding_site	75	93	.	+	0	ID=annotation1964073;Name=LasR Binding Site;Parent=BBa_K091117
BBa_S04349	.	ribosome_entry_site	135	146	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04340
BBa_S04349	.	conserved_region	139	142	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04349	.	CDS	153	908	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S04341
BBa_S04349	.	engineered_region	153	908	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04349	.	engineered_region	153	154	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04349	.	CDS	153	902	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04349	.	point_mutation	326	326	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04349	.	point_mutation	644	644	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04349	.	engineered_tag	909	933	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04349	.	ribosome_entry_site	942	953	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04343
BBa_S04349	.	conserved_region	946	949	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04349	.	CDS	960	1577	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_S04344
BBa_S04349	.	CDS	960	1538	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S04349	.	tag	1539	1570	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S04349	.	engineered_region	960	1577	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S04349	.	engineered_tag	1578	1602	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S04349	.	ribosome_entry_site	2830	2841	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S04349	.	conserved_region	2834	2837	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S04349
tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatcta
tctcatttgctagttataaaattatgaaatttgtataaattcttcagtactagagaaagaggagaaatactagatgaaa
aacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgct
tatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctga
tatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctata
gtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaa
atgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcgg
aatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccatta
attgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagag
aaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtac
tgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaatttta
acaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaagagg
agaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaag
tcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgat
aactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacag
gtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaatt
aagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagct
atatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagc
gtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctat
gcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactc
tgatagtgctagtgtagatctctactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcac
cgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccga
ggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagc
tgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaa
gaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgcc
ggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagatt
ccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctg
ccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccg
gaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcag
cccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccc
cggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatc
ggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagagg
atccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggc
ggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgcc
atagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcct
acagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagaaagaggagaaa