LOCUS BBa_S04351 3655 bp DNA linear 05-Nov-2024 DEFINITION S04350:B0010 ACCESSION BBa_S04351 VERSION BBa_S04351.1 FEATURES Location/Qualifiers misc_feature 86..91 /label=-35 Region misc_feature 75..93 /label=LasR Binding Site misc_feature 113..118 /label= -10 Region misc_feature 125..125 /label=Primary Transcriptional Start promoter 1..126 /label=BBa_K091117 misc_feature 139..142 /label=conserved RBS 135..146 /label=BBa_B0034 misc_feature 1..146 /label=BBa_S04340 misc_feature 153..154 /label=prefix misc_feature 326..326 /label=T misc_feature 644..644 /label=A CDS 153..902 /label=luxR misc_feature 153..908 /label=BBa_C0062 misc_feature 909..933 /label=Help:Barcodes CDS 153..908 /label=BBa_C0062 misc_feature 1..933 /label=BBa_S04341 misc_feature 946..949 /label=conserved RBS 942..953 /label=BBa_B0034 misc_feature 1..953 /label=BBa_S04343 misc_feature 1539..1570 /label=LVA CDS 960..1538 /label=luxI misc_feature 960..1577 /label=BBa_C0061 misc_feature 1578..1602 /label=Help:Barcodes CDS 960..1577 /label=BBa_C0061 misc_feature 1..1602 /label=BBa_S04344 misc_feature 1615..1618 /label=conserved RBS 1611..1622 /label=BBa_B0034 misc_feature 1..1622 /label=BBa_S04345 CDS 1631..2821 /label=tetA(C) backwards misc_feature 1631..1633 /label=stop primer_bind 1631..1650 /label=Rev primer J31006 primer_bind 2798..2821 /label=Fwd primer J31006 CDS 1631..2821 /label=BBa_J31006 misc_feature 1..2821 /label=BBa_S04348 misc_feature 2834..2837 /label=conserved RBS 2830..2841 /label=BBa_B0034 misc_feature 1..2841 /label=BBa_S04349 CDS 2848..3567 /label=GFP protein CDS 2848..3567 /label=BBa_E0040 misc_feature 1..3567 /label=BBa_S04350 stem_loop 3587..3630 /label=stem_loop misc_feature 3576..3655 /label=BBa_B0010 terminator 3576..3655 /label=BBa_B0010 ORIGIN 1 tgttctcgtg tgaagccatt gctctgatct tttggacgtt tcttcgagcc tagcaagggt 61 ccgggttcac cgaaatctat ctcatttgct agttataaaa ttatgaaatt tgtataaatt 121 cttcagtact agagaaagag gagaaatact agatgaaaaa cataaatgcc gacgacacat 181 acagaataat taataaaatt aaagcttgta gaagcaataa tgatattaat caatgcttat 241 ctgatatgac taaaatggta cattgtgaat attatttact cgcgatcatt tatcctcatt 301 ctatggttaa atctgatatt tcaatcctag ataattaccc taaaaaatgg aggcaatatt 361 atgatgacgc taatttaata aaatatgatc ctatagtaga ttattctaac tccaatcatt 421 caccaattaa ttggaatata tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa 481 ttaaagaagc gaaaacatca ggtcttatca ctgggtttag tttccctatt catacggcta 541 acaatggctt cggaatgctt agttttgcac attcagaaaa agacaactat atagatagtt 601 tatttttaca tgcgtgtatg aacataccat taattgttcc ttctctagtt gataattatc 661 gaaaaataaa tatagcaaat aataaatcaa acaacgattt aaccaaaaga gaaaaagaat 721 gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata ttaggttgca 781 gtgagcgtac tgtcactttc catttaacca atgcgcaaat gaaactcaat acaacaaacc 841 gctgccaaag tatttctaaa gcaattttaa caggagcaat tgattgccca tactttaaaa 901 attaataaca ctgatagtgc tagtgtagat cactactaga gaaagaggag aaatactaga 961 tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag tataaaggta 1021 ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta gttgtagaaa 1081 ataaccttga atcagatgag tatgataact caaatgcaga atatatttat gcttgtgatg 1141 atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat tatatgctga 1201 aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct aatatagtcg 1261 aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct gctagtgaaa 1321 ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt attacagaat 1381 atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa gttccttgtc 1441 atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt gtattgtcta 1501 tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg 1561 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag aaagaggaga 1621 aatactagag ttaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc 1681 agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg 1741 cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg 1801 cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct 1861 gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc 1921 agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga 1981 taatggcctg cttctcgccg aaacgtttgg tggcggggcc agtgacgaag gcttgagcga 2041 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa 2101 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga 2161 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc 2221 tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca 2281 ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg 2341 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc 2401 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg 2461 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg 2521 cgtagaggat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa 2581 gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg 2641 gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga 2701 tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta 2761 tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca 2821 ttactagaga aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt 2881 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga 2941 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga 3001 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt 3061 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt 3121 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa 3181 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa 3241 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac 3301 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt 3361 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc 3421 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc 3481 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat 3541 ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc 3601 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctc //