LOCUS       BBa_S04358              2886 bp    DNA     linear       29-Dec-2025
DEFINITION  C0062:S04357
ACCESSION   BBa_S04358
VERSION     BBa_S04358.1  
FEATURES             Location/Qualifiers
     misc_feature    1..2
                     /label=prefix
     misc_feature    174..174
                     /label=T
     misc_feature    492..492
                     /label=A
     CDS             1..750
                     /label=luxR
     misc_feature    1..756
                     /label=BBa_C0062
     misc_feature    757..781
                     /label=Help:Barcodes
     CDS             1..756
                     /label=BBa_C0062
     misc_feature    794..797
                     /label=conserved
     RBS             790..801
                     /label=BBa_B0034
     CDS             810..2000
                     /label=tetA(C) backwards
     misc_feature    810..812
                     /label=stop
     primer_bind     810..829
                     /label=Rev primer J31006
     primer_bind     1977..2000
                     /label=Fwd primer J31006
     CDS             810..2000
                     /label=BBa_J31006
     misc_feature    2013..2016
                     /label=conserved
     RBS             2009..2020
                     /label=BBa_B0034
     CDS             2027..2749
                     /label=BBa_E0020
     stem_loop       2769..2812
                     /label=stem_loop
     misc_feature    2758..2837
                     /label=BBa_B0010
     terminator      2758..2837
                     /label=BBa_B0010
     stem_loop       2853..2872
                     /label=T7 TE
     misc_feature    2879..2879
                     /label=stop
     polyA_site      2873..2886
                     /label=polya
     misc_feature    2846..2886
                     /label=BBa_B0012
     terminator      2846..2886
                     /label=BBa_B0012
     misc_feature    790..2886
                     /label=BBa_S04357
ORIGIN
        1 atgaaaaaca taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga
       61 agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat
      121 tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat
      181 aattacccta aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct
      241 atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat
      301 gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact
      361 gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat
      421 tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta
      481 attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac
      541 aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct
      601 tgggatattt caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat
      661 gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca
      721 ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca
      781 ctactagaga aagaggagaa atactagagt taggtcgagg tggcccggct ccatgcaccg
      841 cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg
      901 ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa
      961 gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
     1021 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     1081 ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg
     1141 tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcggggcca
     1201 gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
     1261 atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
     1321 tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc
     1381 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct
     1441 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
     1501 gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
     1561 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     1621 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     1681 ccggccacga tgcgtccggc gtagaggatc cacaggacgg gtgtggtcgc catgatcgcg
     1741 tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg
     1801 gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc
     1861 acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt
     1921 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
     1981 agcgcattgt tagatttcat tactagagaa agaggagaaa tactagatgg tgagcaaggg
     2041 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg
     2101 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2161 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     2221 gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     2281 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2341 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2401 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2461 ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
     2521 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     2581 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     2641 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2701 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaataat actagagcca
     2761 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     2821 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     2881 tttata
//