##gff-version 3
##sequence-region BBa_S04358 1 2886
BBa_S04358	.	CDS	1	756	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_S04358	.	engineered_tag	757	781	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04358	.	engineered_region	1	756	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04358	.	engineered_region	1	2	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04358	.	point_mutation	174	174	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04358	.	CDS	1	750	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04358	.	point_mutation	492	492	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04358	.	engineered_region	790	2886	.	+	0	ID=BBa_S04357;Name=BBa_S04357
BBa_S04358	.	terminator	2846	2886	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S04357
BBa_S04358	.	stop_codon	2879	2879	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04358	.	polyA_site	2873	2886	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04358	.	stem_loop	2853	2872	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04358	.	engineered_region	2846	2886	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04358	.	CDS	810	2000	.	+	0	ID=BBa_J31006;Name=BBa_J31006;Parent=BBa_S04357
BBa_S04358	.	primer_binding_site	1977	2000	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_S04358	.	stop_codon	810	812	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S04358	.	CDS	810	2000	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S04358	.	primer_binding_site	810	829	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_S04358	.	ribosome_entry_site	2009	2020	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04357
BBa_S04358	.	conserved_region	2013	2016	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04358	.	terminator	2758	2837	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S04357
BBa_S04358	.	engineered_region	2758	2837	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04358	.	stem_loop	2769	2812	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04358	.	CDS	2027	2749	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_S04357
BBa_S04358	.	ribosome_entry_site	790	801	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04357
BBa_S04358	.	conserved_region	794	797	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S04358
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatc
aatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaa
atctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgat
cctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaat
ctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatgg
cttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacata
ccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacca
aaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtga
gcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagca
attttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagaga
aagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggta
tagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggt
ccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctgg
acagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcg
cgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgt
ttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcg
tcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaa
gaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaag
ggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccg
ccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgcc
gaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaacc
gcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcg
cgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaac
gggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacg
atatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatga
cgatgagcgcattgttagatttcattactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttca
ccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgaggg
cgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtg
accaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgcca
tgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagtt
cgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaag
ctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaaga
tccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgt
gctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtc
ctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata