LOCUS       BBa_S04359              2904 bp    DNA     linear       22-Dec-2024
DEFINITION  B0034:S04358
ACCESSION   BBa_S04359
VERSION     BBa_S04359.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..20
                     /label=prefix
     misc_feature    192..192
                     /label=T
     misc_feature    510..510
                     /label=A
     CDS             19..768
                     /label=luxR
     misc_feature    19..774
                     /label=BBa_C0062
     misc_feature    775..799
                     /label=Help:Barcodes
     CDS             19..774
                     /label=BBa_C0062
     misc_feature    812..815
                     /label=conserved
     RBS             808..819
                     /label=BBa_B0034
     CDS             828..2018
                     /label=tetA(C) backwards
     misc_feature    828..830
                     /label=stop
     primer_bind     828..847
                     /label=Rev primer J31006
     primer_bind     1995..2018
                     /label=Fwd primer J31006
     CDS             828..2018
                     /label=BBa_J31006
     misc_feature    2031..2034
                     /label=conserved
     RBS             2027..2038
                     /label=BBa_B0034
     CDS             2045..2767
                     /label=BBa_E0020
     stem_loop       2787..2830
                     /label=stem_loop
     misc_feature    2776..2855
                     /label=BBa_B0010
     terminator      2776..2855
                     /label=BBa_B0010
     stem_loop       2871..2890
                     /label=T7 TE
     misc_feature    2897..2897
                     /label=stop
     polyA_site      2891..2904
                     /label=polya
     misc_feature    2864..2904
                     /label=BBa_B0012
     terminator      2864..2904
                     /label=BBa_B0012
     misc_feature    808..2904
                     /label=BBa_S04357
     misc_feature    19..2904
                     /label=BBa_S04358
ORIGIN
        1 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat
       61 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      121 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
      181 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
      241 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
      301 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
      361 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
      421 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
      481 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
      541 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
      601 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
      661 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
      721 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga
      781 tagtgctagt gtagatcact actagagaaa gaggagaaat actagagtta ggtcgaggtg
      841 gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct
      901 acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc
      961 agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc
     1021 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg
     1081 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc
     1141 aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa
     1201 cgtttggtgg cggggccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc
     1261 gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc
     1321 cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg
     1381 gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
     1441 ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     1501 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     1561 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     1621 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     1681 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcca caggacgggt
     1741 gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg
     1801 cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac
     1861 gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc
     1921 cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg
     1981 gtgccgagga tgacgatgag cgcattgtta gatttcatta ctagagaaag aggagaaata
     2041 ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2101 ggacggcgac gtgaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2161 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2221 caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat
     2281 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2341 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2401 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2461 gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa
     2521 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2581 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2641 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     2701 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     2761 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2821 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2881 cgggtgggcc tttctgcgtt tata
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