##gff-version 3
##sequence-region BBa_S04359 1 2904
BBa_S04359	.	engineered_region	19	2904	.	+	0	ID=BBa_S04358;Name=BBa_S04358
BBa_S04359	.	CDS	19	774	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S04358
BBa_S04359	.	CDS	19	768	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04359	.	engineered_region	19	774	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04359	.	engineered_region	19	20	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04359	.	point_mutation	510	510	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04359	.	engineered_tag	775	799	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04359	.	point_mutation	192	192	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04359	.	engineered_region	808	2904	.	+	0	ID=BBa_S04357;Name=BBa_S04357;Parent=BBa_S04358
BBa_S04359	.	CDS	2045	2767	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_S04357
BBa_S04359	.	terminator	2776	2855	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S04357
BBa_S04359	.	engineered_region	2776	2855	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04359	.	stem_loop	2787	2830	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04359	.	ribosome_entry_site	808	819	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04357
BBa_S04359	.	conserved_region	812	815	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04359	.	ribosome_entry_site	2027	2038	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04357
BBa_S04359	.	conserved_region	2031	2034	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04359	.	CDS	828	2018	.	+	0	ID=BBa_J31006;Name=BBa_J31006;Parent=BBa_S04357
BBa_S04359	.	primer_binding_site	1995	2018	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_S04359	.	primer_binding_site	828	847	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_S04359	.	CDS	828	2018	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S04359	.	stop_codon	828	830	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S04359	.	terminator	2864	2904	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S04357
BBa_S04359	.	engineered_region	2864	2904	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04359	.	stem_loop	2871	2890	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04359	.	stop_codon	2897	2897	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04359	.	polyA_site	2891	2904	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04359	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S04359	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S04359
aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaa
gcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattta
tcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgct
aatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaaca
atgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctat
tcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttta
catgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaat
caaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaa
aatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgc
caaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagt
gtagatcactactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgc
ggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatc
gccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggt
cgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggg
gaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcc
tgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaa
gcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcc
tacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgact
gggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggt
tgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcc
tgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcg
atataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgg
gtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtc
ggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcg
atgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccaggg
tgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagaaagaggagaaatactagatggtgagca
agggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgt
gtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtg
ccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacg
acttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagac
ccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggc
aacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggca
tcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccc
catcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgag
aagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaat
aatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata