LOCUS BBa_S04360 3693 bp DNA linear 22-Nov-2024 DEFINITION C0062:S04359 ACCESSION BBa_S04360 VERSION BBa_S04360.1 FEATURES Location/Qualifiers misc_feature 1..2 /label=prefix misc_feature 174..174 /label=T misc_feature 492..492 /label=A CDS 1..750 /label=luxR misc_feature 1..756 /label=BBa_C0062 misc_feature 757..781 /label=Help:Barcodes CDS 1..756 /label=BBa_C0062 misc_feature 794..797 /label=conserved RBS 790..801 /label=BBa_B0034 misc_feature 808..809 /label=prefix misc_feature 981..981 /label=T misc_feature 1299..1299 /label=A CDS 808..1557 /label=luxR misc_feature 808..1563 /label=BBa_C0062 misc_feature 1564..1588 /label=Help:Barcodes CDS 808..1563 /label=BBa_C0062 misc_feature 1601..1604 /label=conserved RBS 1597..1608 /label=BBa_B0034 CDS 1617..2807 /label=tetA(C) backwards misc_feature 1617..1619 /label=stop primer_bind 1617..1636 /label=Rev primer J31006 primer_bind 2784..2807 /label=Fwd primer J31006 CDS 1617..2807 /label=BBa_J31006 misc_feature 2820..2823 /label=conserved RBS 2816..2827 /label=BBa_B0034 CDS 2834..3556 /label=BBa_E0020 stem_loop 3576..3619 /label=stem_loop misc_feature 3565..3644 /label=BBa_B0010 terminator 3565..3644 /label=BBa_B0010 stem_loop 3660..3679 /label=T7 TE misc_feature 3686..3686 /label=stop polyA_site 3680..3693 /label=polya misc_feature 3653..3693 /label=BBa_B0012 terminator 3653..3693 /label=BBa_B0012 misc_feature 1597..3693 /label=BBa_S04357 misc_feature 808..3693 /label=BBa_S04358 misc_feature 790..3693 /label=BBa_S04359 ORIGIN 1 atgaaaaaca taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga 61 agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat 121 tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat 181 aattacccta aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct 241 atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat 301 gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact 361 gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat 421 tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta 481 attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac 541 aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct 601 tgggatattt caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat 661 gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca 721 ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca 781 ctactagaga aagaggagaa atactagatg aaaaacataa atgccgacga cacatacaga 841 ataattaata aaattaaagc ttgtagaagc aataatgata ttaatcaatg cttatctgat 901 atgactaaaa tggtacattg tgaatattat ttactcgcga tcatttatcc tcattctatg 961 gttaaatctg atatttcaat cctagataat taccctaaaa aatggaggca atattatgat 1021 gacgctaatt taataaaata tgatcctata gtagattatt ctaactccaa tcattcacca 1081 attaattgga atatatttga aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa 1141 gaagcgaaaa catcaggtct tatcactggg tttagtttcc ctattcatac ggctaacaat 1201 ggcttcggaa tgcttagttt tgcacattca gaaaaagaca actatataga tagtttattt 1261 ttacatgcgt gtatgaacat accattaatt gttccttctc tagttgataa ttatcgaaaa 1321 ataaatatag caaataataa atcaaacaac gatttaacca aaagagaaaa agaatgttta 1381 gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa aaatattagg ttgcagtgag 1441 cgtactgtca ctttccattt aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc 1501 caaagtattt ctaaagcaat tttaacagga gcaattgatt gcccatactt taaaaattaa 1561 taacactgat agtgctagtg tagatcacta ctagagaaag aggagaaata ctagagttag 1621 gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg 1681 gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc 1741 gtgacgatca gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga 1801 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc 1861 ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg 1921 aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc 1981 tcgccgaaac gtttggtggc ggggccagtg acgaaggctt gagcgagggc gtgcaagatt 2041 ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg 2101 aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc 2161 ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag 2221 gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc 2281 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg 2341 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc 2401 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca 2461 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatccac 2521 aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc 2581 aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat 2641 tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg 2701 acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc 2761 agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcattac tagagaaaga 2821 ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 2881 ggtcgagctg gacggcgacg tgaacggcca caagttcagc gtgtccggcg agggcgaggg 2941 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 3001 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc 3061 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 3121 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 3181 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 3241 catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga 3301 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag 3361 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 3421 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 3481 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 3541 gctgtacaag taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 3601 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 3661 gctcaccttc gggtgggcct ttctgcgttt ata //