LOCUS BBa_S04361 3711 bp DNA linear 22-Dec-2024 DEFINITION B0034:S04360 ACCESSION BBa_S04361 VERSION BBa_S04361.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 19..20 /label=prefix misc_feature 192..192 /label=T misc_feature 510..510 /label=A CDS 19..768 /label=luxR misc_feature 19..774 /label=BBa_C0062 misc_feature 775..799 /label=Help:Barcodes CDS 19..774 /label=BBa_C0062 misc_feature 812..815 /label=conserved RBS 808..819 /label=BBa_B0034 misc_feature 826..827 /label=prefix misc_feature 999..999 /label=T misc_feature 1317..1317 /label=A CDS 826..1575 /label=luxR misc_feature 826..1581 /label=BBa_C0062 misc_feature 1582..1606 /label=Help:Barcodes CDS 826..1581 /label=BBa_C0062 misc_feature 1619..1622 /label=conserved RBS 1615..1626 /label=BBa_B0034 CDS 1635..2825 /label=tetA(C) backwards misc_feature 1635..1637 /label=stop primer_bind 1635..1654 /label=Rev primer J31006 primer_bind 2802..2825 /label=Fwd primer J31006 CDS 1635..2825 /label=BBa_J31006 misc_feature 2838..2841 /label=conserved RBS 2834..2845 /label=BBa_B0034 CDS 2852..3574 /label=BBa_E0020 stem_loop 3594..3637 /label=stem_loop misc_feature 3583..3662 /label=BBa_B0010 terminator 3583..3662 /label=BBa_B0010 stem_loop 3678..3697 /label=T7 TE misc_feature 3704..3704 /label=stop polyA_site 3698..3711 /label=polya misc_feature 3671..3711 /label=BBa_B0012 terminator 3671..3711 /label=BBa_B0012 misc_feature 1615..3711 /label=BBa_S04357 misc_feature 826..3711 /label=BBa_S04358 misc_feature 808..3711 /label=BBa_S04359 misc_feature 19..3711 /label=BBa_S04360 ORIGIN 1 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat 61 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa 121 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct 181 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat 241 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg 301 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa 361 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga 421 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg 481 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata 541 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca 601 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc 661 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt 721 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga 781 tagtgctagt gtagatcact actagagaaa gaggagaaat actagatgaa aaacataaat 841 gccgacgaca catacagaat aattaataaa attaaagctt gtagaagcaa taatgatatt 901 aatcaatgct tatctgatat gactaaaatg gtacattgtg aatattattt actcgcgatc 961 atttatcctc attctatggt taaatctgat atttcaatcc tagataatta ccctaaaaaa 1021 tggaggcaat attatgatga cgctaattta ataaaatatg atcctatagt agattattct 1081 aactccaatc attcaccaat taattggaat atatttgaaa acaatgctgt aaataaaaaa 1141 tctccaaatg taattaaaga agcgaaaaca tcaggtctta tcactgggtt tagtttccct 1201 attcatacgg ctaacaatgg cttcggaatg cttagttttg cacattcaga aaaagacaac 1261 tatatagata gtttattttt acatgcgtgt atgaacatac cattaattgt tccttctcta 1321 gttgataatt atcgaaaaat aaatatagca aataataaat caaacaacga tttaaccaaa 1381 agagaaaaag aatgtttagc gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa 1441 atattaggtt gcagtgagcg tactgtcact ttccatttaa ccaatgcgca aatgaaactc 1501 aatacaacaa accgctgcca aagtatttct aaagcaattt taacaggagc aattgattgc 1561 ccatacttta aaaattaata acactgatag tgctagtgta gatcactact agagaaagag 1621 gagaaatact agagttaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg 1681 aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc 1741 gaggcggcat aaatcgccgt gacgatcagc ggtccaatga tcgaagttag gctggtaaga 1801 gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg 1861 gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc 1921 atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg 1981 gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg ggccagtgac gaaggcttga 2041 gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag 2101 cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc 2161 atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag 2221 gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc 2281 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat 2341 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc 2401 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg 2461 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt 2521 ccggcgtaga ggatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct 2581 ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga 2641 acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg 2701 gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag 2761 cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat 2821 ttcattacta gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac 2881 cggggtggtg cccatcctgg tcgagctgga cggcgacgtg aacggccaca agttcagcgt 2941 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 3001 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca 3061 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 3121 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 3181 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 3241 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa 3301 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca 3361 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 3421 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 3481 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 3541 cactctcggc atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac 3601 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 3661 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //