##gff-version 3
##sequence-region BBa_S04361 1 3711
BBa_S04361	.	engineered_region	19	3711	.	+	0	ID=BBa_S04360;Name=BBa_S04360
BBa_S04361	.	engineered_region	808	3711	.	+	0	ID=BBa_S04359;Name=BBa_S04359;Parent=BBa_S04360
BBa_S04361	.	engineered_region	826	3711	.	+	0	ID=BBa_S04358;Name=BBa_S04358;Parent=BBa_S04359
BBa_S04361	.	engineered_region	1615	3711	.	+	0	ID=BBa_S04357;Name=BBa_S04357;Parent=BBa_S04358
BBa_S04361	.	CDS	1635	2825	.	+	0	ID=BBa_J31006;Name=BBa_J31006;Parent=BBa_S04357
BBa_S04361	.	CDS	1635	2825	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S04361	.	stop_codon	1635	1637	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S04361	.	primer_binding_site	2802	2825	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_S04361	.	primer_binding_site	1635	1654	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_S04361	.	CDS	2852	3574	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_S04357
BBa_S04361	.	ribosome_entry_site	2834	2845	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04357
BBa_S04361	.	conserved_region	2838	2841	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04361	.	ribosome_entry_site	1615	1626	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04357
BBa_S04361	.	conserved_region	1619	1622	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04361	.	terminator	3671	3711	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S04357
BBa_S04361	.	stop_codon	3704	3704	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04361	.	engineered_region	3671	3711	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04361	.	stem_loop	3678	3697	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04361	.	polyA_site	3698	3711	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04361	.	terminator	3583	3662	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S04357
BBa_S04361	.	stem_loop	3594	3637	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04361	.	engineered_region	3583	3662	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04361	.	CDS	826	1581	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S04358
BBa_S04361	.	engineered_region	826	1581	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04361	.	CDS	826	1575	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04361	.	engineered_region	826	827	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04361	.	point_mutation	999	999	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04361	.	engineered_tag	1582	1606	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04361	.	point_mutation	1317	1317	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04361	.	ribosome_entry_site	808	819	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04359
BBa_S04361	.	conserved_region	812	815	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04361	.	CDS	19	774	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S04360
BBa_S04361	.	engineered_region	19	774	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04361	.	CDS	19	768	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04361	.	engineered_region	19	20	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04361	.	point_mutation	192	192	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04361	.	engineered_tag	775	799	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04361	.	point_mutation	510	510	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04361	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S04361	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S04361
aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaa
gcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattta
tcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgct
aatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaaca
atgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctat
tcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttta
catgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaat
caaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaa
aatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgc
caaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagt
gtagatcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataa
aattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattat
ttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggc
aatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattg
gaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcact
gggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactata
tagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaa
tatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagc
tcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactca
atacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaata
acactgatagtgctagtgtagatcactactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccat
gcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcg
ccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttg
aagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcg
agaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgcca
tgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaa
gattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagc
gctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgccc
accggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaa
gcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagt
cccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccc
catcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgta
gaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactggg
cggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagca
cgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctat
gcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagaaagaggagaa
atactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaac
ggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcacca
ccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccga
ccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggac
gacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcg
acttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccga
caagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccac
taccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctga
gcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatgga
cgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata