BBa_S04361
type: ComponentDefinition
persistent identity: BBa S04361
display id: BBa_S04361
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:S04360
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_S04360
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_S04360
sequence: https://synbiohub.org/public/igem/BBa_S04361_sequence/1

BBa_S04360
type: Component
persistent identity: component2288289
display id: component2288289
version: 1
top level: BBa_S04361
definition: BBa_S04360
access: public

BBa_B0034
type: Component
persistent identity: component2288255
display id: component2288255
version: 1
top level: BBa_S04361
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2288255
display id: annotation2288255
version: 1
top level: BBa_S04361
location: https://synbiohub.org/public/igem/BBa_S04361/annotation2288255/range2288255/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04361/annotation2288255/range2288255/1
type: Range
persistent identity: range2288255
display id: range2288255
version: 1
top level: BBa_S04361
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_S04360
type: SequenceAnnotation
persistent identity: annotation2288289
display id: annotation2288289
version: 1
top level: BBa_S04361
location: https://synbiohub.org/public/igem/BBa_S04361/annotation2288289/range2288289/1
component: BBa_S04360

https://synbiohub.org/public/igem/BBa_S04361/annotation2288289/range2288289/1
type: Range
persistent identity: range2288289
display id: range2288289
version: 1
top level: BBa_S04361
direction: direction/reverse
start: 19
end: 3711
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_S04360
type: ComponentDefinition
persistent identity: BBa S04360
display id: BBa_S04360
version: 1
was generated by: iGEM to SBOL conversion
description: C0062:S04359
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S04359
component: BBa_C0062
sequence annotation: BBa_C0062
sequence annotation: BBa_S04359
sequence: https://synbiohub.org/public/igem/BBa_S04360_sequence/1

BBa_S04359
type: Component
persistent identity: component2277052
display id: component2277052
version: 1
top level: BBa_S04360
definition: BBa_S04359
access: public

BBa_C0062
type: Component
persistent identity: component2277022
display id: component2277022
version: 1
top level: BBa_S04360
definition: luxr
access: public

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2277022
display id: annotation2277022
version: 1
top level: BBa_S04360
location: https://synbiohub.org/public/igem/BBa_S04360/annotation2277022/range2277022/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_S04360/annotation2277022/range2277022/1
type: Range
persistent identity: range2277022
display id: range2277022
version: 1
top level: BBa_S04360
direction: direction/forward
start: 1
end: 756
orientation: inline

BBa_S04359
type: SequenceAnnotation
persistent identity: annotation2277052
display id: annotation2277052
version: 1
top level: BBa_S04360
location: https://synbiohub.org/public/igem/BBa_S04360/annotation2277052/range2277052/1
component: BBa_S04359

https://synbiohub.org/public/igem/BBa_S04360/annotation2277052/range2277052/1
type: Range
persistent identity: range2277052
display id: range2277052
version: 1
top level: BBa_S04360
direction: direction/reverse
start: 790
end: 3693
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

BBa_S04359
type: ComponentDefinition
persistent identity: BBa S04359
display id: BBa_S04359
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:S04358
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_S04358
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_S04358
sequence: https://synbiohub.org/public/igem/BBa_S04359_sequence/1

BBa_S04358
type: Component
persistent identity: component2272282
display id: component2272282
version: 1
top level: BBa_S04359
definition: BBa_S04358
access: public

BBa_B0034
type: Component
persistent identity: component2272258
display id: component2272258
version: 1
top level: BBa_S04359
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2272258
display id: annotation2272258
version: 1
top level: BBa_S04359
location: https://synbiohub.org/public/igem/BBa_S04359/annotation2272258/range2272258/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04359/annotation2272258/range2272258/1
type: Range
persistent identity: range2272258
display id: range2272258
version: 1
top level: BBa_S04359
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_S04358
type: SequenceAnnotation
persistent identity: annotation2272282
display id: annotation2272282
version: 1
top level: BBa_S04359
location: https://synbiohub.org/public/igem/BBa_S04359/annotation2272282/range2272282/1
component: BBa_S04358

https://synbiohub.org/public/igem/BBa_S04359/annotation2272282/range2272282/1
type: Range
persistent identity: range2272282
display id: range2272282
version: 1
top level: BBa_S04359
direction: direction/reverse
start: 19
end: 2904
orientation: inline

BBa_S04358
type: ComponentDefinition
persistent identity: BBa S04358
display id: BBa_S04358
version: 1
was generated by: iGEM to SBOL conversion
description: C0062:S04357
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_S04357
component: BBa_C0062
sequence annotation: BBa_C0062
sequence annotation: BBa_S04357
sequence: https://synbiohub.org/public/igem/BBa_S04358_sequence/1

BBa_S04357
type: Component
persistent identity: component2267991
display id: component2267991
version: 1
top level: BBa_S04358
definition: BBa_S04357
access: public

BBa_C0062
type: Component
persistent identity: component2267971
display id: component2267971
version: 1
top level: BBa_S04358
definition: luxr
access: public

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2267971
display id: annotation2267971
version: 1
top level: BBa_S04358
location: https://synbiohub.org/public/igem/BBa_S04358/annotation2267971/range2267971/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_S04358/annotation2267971/range2267971/1
type: Range
persistent identity: range2267971
display id: range2267971
version: 1
top level: BBa_S04358
direction: direction/forward
start: 1
end: 756
orientation: inline

BBa_S04357
type: SequenceAnnotation
persistent identity: annotation2267991
display id: annotation2267991
version: 1
top level: BBa_S04358
location: https://synbiohub.org/public/igem/BBa_S04358/annotation2267991/range2267991/1
component: BBa_S04357

https://synbiohub.org/public/igem/BBa_S04358/annotation2267991/range2267991/1
type: Range
persistent identity: range2267991
display id: range2267991
version: 1
top level: BBa_S04358
direction: direction/reverse
start: 790
end: 2886
orientation: inline

BBa_S04357
type: ComponentDefinition
persistent identity: BBa S04357
display id: BBa_S04357
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:S04356
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_J31006
component: BBa_B0034
component: BBa_E0020
sequence annotation: BBa_B0034
sequence annotation: BBa_J31006
sequence annotation: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S04357_sequence/1

BBa_B0012
type: Component
persistent identity: component2063308
display id: component2063308
version: 1
top level: BBa_S04357
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2063306
display id: component2063306
version: 1
top level: BBa_S04357
definition: BBa_B0010
access: public

BBa_J31006
type: Component
persistent identity: component2063302
display id: component2063302
version: 1
top level: BBa_S04357
definition: tetA(C)b
access: public

BBa_B0034
type: Component
persistent identity: component2063297
display id: component2063297
version: 1
top level: BBa_S04357
definition: BBa_B0034
access: public

BBa_E0020
type: Component
persistent identity: component2063305
display id: component2063305
version: 1
top level: BBa_S04357
definition: ecfp
access: public

BBa_B0034
type: Component
persistent identity: component2063304
display id: component2063304
version: 1
top level: BBa_S04357
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2063297
display id: annotation2063297
version: 1
top level: BBa_S04357
location: https://synbiohub.org/public/igem/BBa_S04357/annotation2063297/range2063297/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04357/annotation2063297/range2063297/1
type: Range
persistent identity: range2063297
display id: range2063297
version: 1
top level: BBa_S04357
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_J31006
type: SequenceAnnotation
persistent identity: annotation2063302
display id: annotation2063302
version: 1
top level: BBa_S04357
location: https://synbiohub.org/public/igem/BBa_S04357/annotation2063302/range2063302/1
component: BBa_J31006

https://synbiohub.org/public/igem/BBa_S04357/annotation2063302/range2063302/1
type: Range
persistent identity: range2063302
display id: range2063302
version: 1
top level: BBa_S04357
direction: direction/reverse
start: 21
end: 1211
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2063304
display id: annotation2063304
version: 1
top level: BBa_S04357
location: https://synbiohub.org/public/igem/BBa_S04357/annotation2063304/range2063304/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04357/annotation2063304/range2063304/1
type: Range
persistent identity: range2063304
display id: range2063304
version: 1
top level: BBa_S04357
direction: direction/reverse
start: 1220
end: 1231
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation2063305
display id: annotation2063305
version: 1
top level: BBa_S04357
location: https://synbiohub.org/public/igem/BBa_S04357/annotation2063305/range2063305/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_S04357/annotation2063305/range2063305/1
type: Range
persistent identity: range2063305
display id: range2063305
version: 1
top level: BBa_S04357
direction: direction/reverse
start: 1238
end: 1960
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2063306
display id: annotation2063306
version: 1
top level: BBa_S04357
location: https://synbiohub.org/public/igem/BBa_S04357/annotation2063306/range2063306/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S04357/annotation2063306/range2063306/1
type: Range
persistent identity: range2063306
display id: range2063306
version: 1
top level: BBa_S04357
direction: direction/forward
start: 1969
end: 2048
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2063308
display id: annotation2063308
version: 1
top level: BBa_S04357
location: https://synbiohub.org/public/igem/BBa_S04357/annotation2063308/range2063308/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S04357/annotation2063308/range2063308/1
type: Range
persistent identity: range2063308
display id: range2063308
version: 1
top level: BBa_S04357
direction: direction/forward
start: 2057
end: 2097
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: iGEM to SBOL conversion
description: engineered cyan fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

tetA(C)b
type: ComponentDefinition
persistent identity: BBa J31006
display id: BBa_J31006
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This is the coding region for Tetracyline resistance in the reverse orientation. If a promoter adn RBS are placed downstream and the entire region, from promoter to stop codon is flipped the cell will express tetracycline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plasmids.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: stop
sequence annotation: tetA(C) backwards
sequence annotation: Rev primer J31006
sequence annotation: Fwd primer J31006
sequence: https://synbiohub.org/public/igem/BBa_J31006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1910574
display id: annotation1910574
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
type: Range
persistent identity: range1910574
display id: range1910574
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1
end: 3
orientation: inline

tetA(C) backwards
type: SequenceAnnotation
persistent identity: annotation1884998
display id: annotation1884998
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
type: Range
persistent identity: range1884998
display id: range1884998
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1
end: 1191
orientation: inline

Rev primer J31006
type: SequenceAnnotation
persistent identity: annotation1910575
display id: annotation1910575
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
type: Range
persistent identity: range1910575
display id: range1910575
version: 1
top level: tetA(C)b
direction: direction/forward
start: 1
end: 20
orientation: inline

Fwd primer J31006
type: SequenceAnnotation
persistent identity: annotation1910576
display id: annotation1910576
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
type: Range
persistent identity: range1910576
display id: range1910576
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1168
end: 1191
orientation: inline

https://synbiohub.org/public/igem/BBa_S04361_sequence/1
type: Sequence
persistent identity: BBa S04361 sequence
display id: BBa_S04361_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04360_sequence/1
type: Sequence
persistent identity: BBa S04360 sequence
display id: BBa_S04360_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04359_sequence/1
type: Sequence
persistent identity: BBa S04359 sequence
display id: BBa_S04359_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04358_sequence/1
type: Sequence
persistent identity: BBa S04358 sequence
display id: BBa_S04358_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04357_sequence/1
type: Sequence
persistent identity: BBa S04357 sequence
display id: BBa_S04357_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0020_sequence/1
type: Sequence
persistent identity: BBa E0020 sequence
display id: BBa_E0020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31006_sequence/1
type: Sequence
persistent identity: BBa J31006 sequence
display id: BBa_J31006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z