##gff-version 3
##sequence-region BBa_S04362 1 3845
BBa_S04362	.	engineered_region	135	3845	.	+	0	ID=BBa_S04361;Name=BBa_S04361
BBa_S04362	.	engineered_region	153	3845	.	+	0	ID=BBa_S04360;Name=BBa_S04360;Parent=BBa_S04361
BBa_S04362	.	engineered_region	942	3845	.	+	0	ID=BBa_S04359;Name=BBa_S04359;Parent=BBa_S04360
BBa_S04362	.	engineered_region	960	3845	.	+	0	ID=BBa_S04358;Name=BBa_S04358;Parent=BBa_S04359
BBa_S04362	.	CDS	960	1715	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S04358
BBa_S04362	.	CDS	960	1709	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04362	.	engineered_region	960	1715	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04362	.	engineered_tag	1716	1740	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04362	.	point_mutation	1133	1133	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04362	.	engineered_region	960	961	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04362	.	point_mutation	1451	1451	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04362	.	engineered_region	1749	3845	.	+	0	ID=BBa_S04357;Name=BBa_S04357;Parent=BBa_S04358
BBa_S04362	.	CDS	1769	2959	.	+	0	ID=BBa_J31006;Name=BBa_J31006;Parent=BBa_S04357
BBa_S04362	.	stop_codon	1769	1771	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S04362	.	primer_binding_site	2936	2959	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_S04362	.	CDS	1769	2959	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S04362	.	primer_binding_site	1769	1788	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_S04362	.	ribosome_entry_site	2968	2979	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04357
BBa_S04362	.	conserved_region	2972	2975	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04362	.	CDS	2986	3708	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_S04357
BBa_S04362	.	terminator	3717	3796	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S04357
BBa_S04362	.	stem_loop	3728	3771	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04362	.	engineered_region	3717	3796	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04362	.	ribosome_entry_site	1749	1760	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04357
BBa_S04362	.	conserved_region	1753	1756	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04362	.	terminator	3805	3845	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S04357
BBa_S04362	.	engineered_region	3805	3845	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04362	.	stop_codon	3838	3838	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04362	.	stem_loop	3812	3831	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04362	.	polyA_site	3832	3845	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04362	.	ribosome_entry_site	942	953	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04359
BBa_S04362	.	conserved_region	946	949	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04362	.	CDS	153	908	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S04360
BBa_S04362	.	CDS	153	902	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04362	.	engineered_region	153	908	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04362	.	engineered_tag	909	933	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04362	.	point_mutation	326	326	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04362	.	engineered_region	153	154	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04362	.	point_mutation	644	644	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04362	.	ribosome_entry_site	135	146	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04361
BBa_S04362	.	conserved_region	139	142	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04362	.	promoter	1	126	.	+	0	ID=BBa_K091146;Name=BBa_K091146
BBa_S04362	.	start_codon	125	125	.	+	0	ID=annotation1967358;Name=Transcriptional Start;Parent=BBa_K091146
BBa_S04362	.	sequence_feature	86	91	.	+	0	ID=annotation1967357;Name=-35;Parent=BBa_K091146
BBa_S04362	.	non_covalent_binding_site	94	113	.	+	0	ID=annotation1967355;Name=LuxR;Parent=BBa_K091146
BBa_S04362	.	sequence_feature	113	118	.	+	0	ID=annotation1967356;Name=-10;Parent=BBa_K091146
BBa_S04362	.	non_covalent_binding_site	75	93	.	+	0	ID=annotation1967354;Name=LasR;Parent=BBa_K091146
>BBa_S04362
tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatcta
tctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatgaaa
aacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgct
tatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctga
tatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctata
gtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaa
atgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcgg
aatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccatta
attgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagag
aaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtac
tgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaatttta
acaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaagagg
agaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataa
tgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcat
tctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaa
taaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgt
aaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacg
gctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgt
gtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaa
cgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatatta
ggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagta
tttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatc
actactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggagg
cagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtga
cgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatc
tacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggcc
atccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttct
cgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacag
gccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagt
tgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttga
aggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggcc
gttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccacc
atacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatatagg
cgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggt
cgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagt
gctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgt
cggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggt
gccgaggatgacgatgagcgcattgttagatttcattactagagaaagaggagaaatactagatggtgagcaagggcga
ggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggc
gagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggc
ccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttctt
caagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgcc
gaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcc
tggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggc
caacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggc
gacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcg
atcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata