LOCUS       BBa_S04369              2845 bp    DNA     linear       05-Nov-2024
DEFINITION  S04368:J31006
ACCESSION   BBa_S04369
VERSION     BBa_S04369.1  
FEATURES             Location/Qualifiers
     misc_feature    75..93
                     /label=LasR
     misc_feature    94..113
                     /label=LuxR
     misc_feature    113..118
                     /label=-10
     misc_feature    86..91
                     /label=-35
     misc_feature    125..125
                     /label=Transcriptional Start
     promoter        1..126
                     /label=BBa_K091146
     misc_feature    139..142
                     /label=conserved
     RBS             135..146
                     /label=BBa_B0034
     misc_feature    1..146
                     /label=BBa_S04363
     CDS             153..869
                     /label=lasR
     misc_feature    870..902
                     /label=LVA
     misc_feature    632..632
                     /label=G
     misc_feature    909..933
                     /label=Help:Barcodes
     CDS             153..933
                     /label=BBa_C0079
     misc_feature    1..933
                     /label=BBa_S04364
     misc_feature    946..949
                     /label=conserved
     RBS             942..953
                     /label=BBa_B0034
     misc_feature    1..953
                     /label=BBa_S04365
     CDS             960..1562
                     /label=LasI
     misc_feature    1563..1595
                     /label=LVA
     misc_feature    1602..1626
                     /label=Help:Barcodes
     CDS             960..1626
                     /label=BBa_C0078
     misc_feature    1..1626
                     /label=BBa_S04366
     misc_feature    1639..1642
                     /label=conserved
     RBS             1635..1646
                     /label=BBa_B0034
     misc_feature    1..1646
                     /label=BBa_S04368
     CDS             1655..2845
                     /label=tetA(C) backwards
     misc_feature    1655..1657
                     /label=stop
     primer_bind     1655..1674
                     /label=Rev primer J31006
     primer_bind     2822..2845
                     /label=Fwd primer J31006
     CDS             1655..2845
                     /label=BBa_J31006
ORIGIN
        1 tgttctcgtg tgaagccatt gctctgatct tttggacgtt tcttcgagcc tagcaagggt
       61 ccgggttcac cgaaatctat ctcatttgct agtacctgta ggatcgtaca ggtataaatt
      121 cttcagtact agagaaagag gagaaatact agatggcctt ggttgacggt tttcttgagc
      181 tggaacgctc aagtggaaaa ttggagtgga gcgccatcct ccagaagatg gcgagcgacc
      241 ttggattctc gaagatcctg ttcggcctgt tgcctaagga cagccaggac tacgagaacg
      301 ccttcatcgt cggcaactac ccggccgcct ggcgcgagca ttacgaccgg gctggctacg
      361 cgcgggtcga cccgacggtc agtcactgta cccagagcgt actgccgatt ttctgggaac
      421 cgtccatcta ccagacgcga aagcagcacg agttcttcga ggaagcctcg gccgccggcc
      481 tggtgtatgg gctgaccatg ccgctgcatg gtgctcgcgg cgaactcggc gcgctgagcc
      541 tcagcgtgga agcggaaaac cgggccgagg ccaaccgttt catagagtcg gtcctgccga
      601 ccctgtggat gctcaaggac tacgcactgc aaagcggtgc cggactggcc ttcgaacatc
      661 cggtcagcaa accggtggtt ctgaccagcc gggagaagga agtgttgcag tggtgcgcca
      721 tcggcaagac cagttgggag atatcggtta tctgcaactg ctcggaagcc aatgtgaact
      781 tccatatggg aaatattcgg cggaagttcg gtgtgacctc ccgccgcgta gcggccatta
      841 tggccgttaa tttgggtctt attactctcg ctgcaaacga cgaaaactac gctttagtag
      901 cttaataaca ctgatagtgc tagtgtagat cactactaga gaaagaggag aaatactaga
      961 tgatcgttca gatcggtcgt cgtgaagagt tcgacaaaaa actgctgggt gaaatgcaca
     1021 aactgcgtgc tcaggttttc aaagaacgta aaggttggga cgtttccgtt atcgacgaaa
     1081 tggaaatcga cggttacgac gctctgtccc cgtactacat gctgatccag gaagacaccc
     1141 cggaagctca ggttttcggt tgctggcgta tcttcgacac caccggtccg tacatgctga
     1201 aaaacacctt cccggaactg ctgcacggta aagaagctcc gtgctccccg cacatctggg
     1261 aactgtcccg tttcgctatc aactccggtc agaaaggttc cctgggtttc tccgactgca
     1321 ccctggaagc tatgcgtgct ctggctcgtt actccttgca gaacgacatc cagaccctgg
     1381 ttaccgttac caccgttggt gttgaaaaaa tgatgatccg tgctggtctg gacgtttccc
     1441 gtttcggtcc gcacctgaaa atcggtatcg aacgtgctgt tgctctgcgt atcgaactga
     1501 acgctaaaac ccagatcgct ctgtacggtg gtgttctggt tgaacagcgt ctggctgttt
     1561 ccgctgctaa cgacgaaaac tacgctctgg ttgcttaata actctgatag tgctagtgta
     1621 gatctctact agagaaagag gagaaatact agagttaggt cgaggtggcc cggctccatg
     1681 caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
     1741 acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccaatga
     1801 tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
     1861 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
     1921 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
     1981 gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     2041 ggccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
     2101 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
     2161 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
     2221 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
     2281 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
     2341 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
     2401 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
     2461 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
     2521 tgatgccggc cacgatgcgt ccggcgtaga ggatccacag gacgggtgtg gtcgccatga
     2581 tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc
     2641 ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta
     2701 gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg
     2761 gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga
     2821 cgatgagcgc attgttagat ttcat
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