LOCUS BBa_S04371 3002 bp DNA linear 22-Nov-2024 DEFINITION S04370:S04353 ACCESSION BBa_S04371 VERSION BBa_S04371.1 FEATURES Location/Qualifiers misc_feature 75..93 /label=LasR misc_feature 94..113 /label=LuxR misc_feature 113..118 /label=-10 misc_feature 86..91 /label=-35 misc_feature 125..125 /label=Transcriptional Start promoter 1..126 /label=BBa_K091146 misc_feature 139..142 /label=conserved RBS 135..146 /label=BBa_B0034 misc_feature 1..146 /label=BBa_S04363 CDS 153..869 /label=lasR misc_feature 870..902 /label=LVA misc_feature 632..632 /label=G misc_feature 909..933 /label=Help:Barcodes CDS 153..933 /label=BBa_C0079 misc_feature 1..933 /label=BBa_S04364 misc_feature 946..949 /label=conserved RBS 942..953 /label=BBa_B0034 misc_feature 1..953 /label=BBa_S04365 CDS 960..1562 /label=LasI misc_feature 1563..1595 /label=LVA misc_feature 1602..1626 /label=Help:Barcodes CDS 960..1626 /label=BBa_C0078 misc_feature 1..1626 /label=BBa_S04366 misc_feature 1639..1642 /label=conserved RBS 1635..1646 /label=BBa_B0034 misc_feature 1..1646 /label=BBa_S04368 CDS 1655..2845 /label=tetA(C) backwards misc_feature 1655..1657 /label=stop primer_bind 1655..1674 /label=Rev primer J31006 primer_bind 2822..2845 /label=Fwd primer J31006 CDS 1655..2845 /label=BBa_J31006 misc_feature 1..2845 /label=BBa_S04369 misc_feature 2858..2861 /label=conserved RBS 2854..2865 /label=BBa_B0034 misc_feature 1..2865 /label=BBa_S04370 stem_loop 2885..2928 /label=stem_loop misc_feature 2874..2953 /label=BBa_B0010 terminator 2874..2953 /label=BBa_B0010 stem_loop 2969..2988 /label=T7 TE misc_feature 2995..2995 /label=stop polyA_site 2989..3002 /label=polya misc_feature 2962..3002 /label=BBa_B0012 terminator 2962..3002 /label=BBa_B0012 misc_feature 2874..3002 /label=BBa_S04353 ORIGIN 1 tgttctcgtg tgaagccatt gctctgatct tttggacgtt tcttcgagcc tagcaagggt 61 ccgggttcac cgaaatctat ctcatttgct agtacctgta ggatcgtaca ggtataaatt 121 cttcagtact agagaaagag gagaaatact agatggcctt ggttgacggt tttcttgagc 181 tggaacgctc aagtggaaaa ttggagtgga gcgccatcct ccagaagatg gcgagcgacc 241 ttggattctc gaagatcctg ttcggcctgt tgcctaagga cagccaggac tacgagaacg 301 ccttcatcgt cggcaactac ccggccgcct ggcgcgagca ttacgaccgg gctggctacg 361 cgcgggtcga cccgacggtc agtcactgta cccagagcgt actgccgatt ttctgggaac 421 cgtccatcta ccagacgcga aagcagcacg agttcttcga ggaagcctcg gccgccggcc 481 tggtgtatgg gctgaccatg ccgctgcatg gtgctcgcgg cgaactcggc gcgctgagcc 541 tcagcgtgga agcggaaaac cgggccgagg ccaaccgttt catagagtcg gtcctgccga 601 ccctgtggat gctcaaggac tacgcactgc aaagcggtgc cggactggcc ttcgaacatc 661 cggtcagcaa accggtggtt ctgaccagcc gggagaagga agtgttgcag tggtgcgcca 721 tcggcaagac cagttgggag atatcggtta tctgcaactg ctcggaagcc aatgtgaact 781 tccatatggg aaatattcgg cggaagttcg gtgtgacctc ccgccgcgta gcggccatta 841 tggccgttaa tttgggtctt attactctcg ctgcaaacga cgaaaactac gctttagtag 901 cttaataaca ctgatagtgc tagtgtagat cactactaga gaaagaggag aaatactaga 961 tgatcgttca gatcggtcgt cgtgaagagt tcgacaaaaa actgctgggt gaaatgcaca 1021 aactgcgtgc tcaggttttc aaagaacgta aaggttggga cgtttccgtt atcgacgaaa 1081 tggaaatcga cggttacgac gctctgtccc cgtactacat gctgatccag gaagacaccc 1141 cggaagctca ggttttcggt tgctggcgta tcttcgacac caccggtccg tacatgctga 1201 aaaacacctt cccggaactg ctgcacggta aagaagctcc gtgctccccg cacatctggg 1261 aactgtcccg tttcgctatc aactccggtc agaaaggttc cctgggtttc tccgactgca 1321 ccctggaagc tatgcgtgct ctggctcgtt actccttgca gaacgacatc cagaccctgg 1381 ttaccgttac caccgttggt gttgaaaaaa tgatgatccg tgctggtctg gacgtttccc 1441 gtttcggtcc gcacctgaaa atcggtatcg aacgtgctgt tgctctgcgt atcgaactga 1501 acgctaaaac ccagatcgct ctgtacggtg gtgttctggt tgaacagcgt ctggctgttt 1561 ccgctgctaa cgacgaaaac tacgctctgg ttgcttaata actctgatag tgctagtgta 1621 gatctctact agagaaagag gagaaatact agagttaggt cgaggtggcc cggctccatg 1681 caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca 1741 acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccaatga 1801 tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat 1861 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa 1921 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca 1981 gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 2041 ggccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc 2101 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg 2161 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca 2221 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac 2281 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga 2341 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca 2401 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc 2461 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg 2521 tgatgccggc cacgatgcgt ccggcgtaga ggatccacag gacgggtgtg gtcgccatga 2581 tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc 2641 ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta 2701 gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg 2761 gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga 2821 cgatgagcgc attgttagat ttcattacta gagaaagagg agaaatacta gagccaggca 2881 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 2941 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 3001 ta // LOCUS BBa_B0015 129 bp DNA linear 22-Nov-2024 DEFINITION double terminator (B0010-B0012) ACCESSION BBa_B0015 VERSION BBa_B0015.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttata //