BBa_S04371
type: ComponentDefinition
persistent identity: BBa S04371
display id: BBa_S04371
version: 1
was generated by: iGEM to SBOL conversion
description: S04370:S04353
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S04353
component: BBa_S04370
sequence annotation: BBa_S04370
sequence annotation: BBa_S04353
sequence: https://synbiohub.org/public/igem/BBa_S04371_sequence/1

BBa_S04353
type: Component
persistent identity: component2288331
display id: component2288331
version: 1
top level: BBa_S04371
definition: BBa_S04353
access: public

BBa_S04370
type: Component
persistent identity: component2288324
display id: component2288324
version: 1
top level: BBa_S04371
definition: BBa_S04370
access: public

BBa_S04370
type: SequenceAnnotation
persistent identity: annotation2288324
display id: annotation2288324
version: 1
top level: BBa_S04371
location: https://synbiohub.org/public/igem/BBa_S04371/annotation2288324/range2288324/1
component: BBa_S04370

https://synbiohub.org/public/igem/BBa_S04371/annotation2288324/range2288324/1
type: Range
persistent identity: range2288324
display id: range2288324
version: 1
top level: BBa_S04371
direction: direction/reverse
start: 1
end: 2865
orientation: inline

BBa_S04353
type: SequenceAnnotation
persistent identity: annotation2288331
display id: annotation2288331
version: 1
top level: BBa_S04371
location: https://synbiohub.org/public/igem/BBa_S04371/annotation2288331/range2288331/1
component: BBa_S04353

https://synbiohub.org/public/igem/BBa_S04371/annotation2288331/range2288331/1
type: Range
persistent identity: range2288331
display id: range2288331
version: 1
top level: BBa_S04371
direction: direction/reverse
start: 2874
end: 3002
orientation: inline

BBa_S04353
type: ComponentDefinition
persistent identity: BBa S04353
display id: BBa_S04353
version: 1
was generated by: iGEM to SBOL conversion
description: B0010:B0012
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: true
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
is replaced by: BBa_B0015
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S04353_sequence/1

BBa_B0012
type: Component
persistent identity: component2063262
display id: component2063262
version: 1
top level: BBa_S04353
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2063260
display id: component2063260
version: 1
top level: BBa_S04353
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2063260
display id: annotation2063260
version: 1
top level: BBa_S04353
location: https://synbiohub.org/public/igem/BBa_S04353/annotation2063260/range2063260/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S04353/annotation2063260/range2063260/1
type: Range
persistent identity: range2063260
display id: range2063260
version: 1
top level: BBa_S04353
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2063262
display id: annotation2063262
version: 1
top level: BBa_S04353
location: https://synbiohub.org/public/igem/BBa_S04353/annotation2063262/range2063262/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S04353/annotation2063262/range2063262/1
type: Range
persistent identity: range2063262
display id: range2063262
version: 1
top level: BBa_S04353
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_S04370
type: ComponentDefinition
persistent identity: BBa S04370
display id: BBa_S04370
version: 1
was generated by: iGEM to SBOL conversion
description: S04369:B0034
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_S04369
sequence annotation: BBa_S04369
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04370_sequence/1

BBa_B0034
type: Component
persistent identity: component2272316
display id: component2272316
version: 1
top level: BBa_S04370
definition: BBa_B0034
access: public

BBa_S04369
type: Component
persistent identity: component2272314
display id: component2272314
version: 1
top level: BBa_S04370
definition: BBa_S04369
access: public

BBa_S04369
type: SequenceAnnotation
persistent identity: annotation2272314
display id: annotation2272314
version: 1
top level: BBa_S04370
location: https://synbiohub.org/public/igem/BBa_S04370/annotation2272314/range2272314/1
component: BBa_S04369

https://synbiohub.org/public/igem/BBa_S04370/annotation2272314/range2272314/1
type: Range
persistent identity: range2272314
display id: range2272314
version: 1
top level: BBa_S04370
direction: direction/reverse
start: 1
end: 2845
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2272316
display id: annotation2272316
version: 1
top level: BBa_S04370
location: https://synbiohub.org/public/igem/BBa_S04370/annotation2272316/range2272316/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04370/annotation2272316/range2272316/1
type: Range
persistent identity: range2272316
display id: range2272316
version: 1
top level: BBa_S04370
direction: direction/reverse
start: 2854
end: 2865
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_S04369
type: ComponentDefinition
persistent identity: BBa S04369
display id: BBa_S04369
version: 1
was generated by: iGEM to SBOL conversion
description: S04368:J31006
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J31006
component: BBa_S04368
sequence annotation: BBa_S04368
sequence annotation: BBa_J31006
sequence: https://synbiohub.org/public/igem/BBa_S04369_sequence/1

BBa_J31006
type: Component
persistent identity: component2268022
display id: component2268022
version: 1
top level: BBa_S04369
definition: tetA(C)b
access: public

BBa_S04368
type: Component
persistent identity: component2268017
display id: component2268017
version: 1
top level: BBa_S04369
definition: BBa_S04368
access: public

BBa_S04368
type: SequenceAnnotation
persistent identity: annotation2268017
display id: annotation2268017
version: 1
top level: BBa_S04369
location: https://synbiohub.org/public/igem/BBa_S04369/annotation2268017/range2268017/1
component: BBa_S04368

https://synbiohub.org/public/igem/BBa_S04369/annotation2268017/range2268017/1
type: Range
persistent identity: range2268017
display id: range2268017
version: 1
top level: BBa_S04369
direction: direction/reverse
start: 1
end: 1646
orientation: inline

BBa_J31006
type: SequenceAnnotation
persistent identity: annotation2268022
display id: annotation2268022
version: 1
top level: BBa_S04369
location: https://synbiohub.org/public/igem/BBa_S04369/annotation2268022/range2268022/1
component: BBa_J31006

https://synbiohub.org/public/igem/BBa_S04369/annotation2268022/range2268022/1
type: Range
persistent identity: range2268022
display id: range2268022
version: 1
top level: BBa_S04369
direction: direction/reverse
start: 1655
end: 2845
orientation: inline

tetA(C)b
type: ComponentDefinition
persistent identity: BBa J31006
display id: BBa_J31006
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This is the coding region for Tetracyline resistance in the reverse orientation. If a promoter adn RBS are placed downstream and the entire region, from promoter to stop codon is flipped the cell will express tetracycline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plasmids.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: stop
sequence annotation: tetA(C) backwards
sequence annotation: Rev primer J31006
sequence annotation: Fwd primer J31006
sequence: https://synbiohub.org/public/igem/BBa_J31006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1910574
display id: annotation1910574
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
type: Range
persistent identity: range1910574
display id: range1910574
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1
end: 3
orientation: inline

tetA(C) backwards
type: SequenceAnnotation
persistent identity: annotation1884998
display id: annotation1884998
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
type: Range
persistent identity: range1884998
display id: range1884998
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1
end: 1191
orientation: inline

Rev primer J31006
type: SequenceAnnotation
persistent identity: annotation1910575
display id: annotation1910575
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
type: Range
persistent identity: range1910575
display id: range1910575
version: 1
top level: tetA(C)b
direction: direction/forward
start: 1
end: 20
orientation: inline

Fwd primer J31006
type: SequenceAnnotation
persistent identity: annotation1910576
display id: annotation1910576
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
type: Range
persistent identity: range1910576
display id: range1910576
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1168
end: 1191
orientation: inline

BBa_S04368
type: ComponentDefinition
persistent identity: BBa S04368
display id: BBa_S04368
version: 1
was generated by: iGEM to SBOL conversion
description: S04366:B0034
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_S04366
sequence annotation: BBa_S04366
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04368_sequence/1

BBa_B0034
type: Component
persistent identity: component2263287
display id: component2263287
version: 1
top level: BBa_S04368
definition: BBa_B0034
access: public

BBa_S04366
type: Component
persistent identity: component2263285
display id: component2263285
version: 1
top level: BBa_S04368
definition: BBa_S04366
access: public

BBa_S04366
type: SequenceAnnotation
persistent identity: annotation2263285
display id: annotation2263285
version: 1
top level: BBa_S04368
location: https://synbiohub.org/public/igem/BBa_S04368/annotation2263285/range2263285/1
component: BBa_S04366

https://synbiohub.org/public/igem/BBa_S04368/annotation2263285/range2263285/1
type: Range
persistent identity: range2263285
display id: range2263285
version: 1
top level: BBa_S04368
direction: direction/reverse
start: 1
end: 1626
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2263287
display id: annotation2263287
version: 1
top level: BBa_S04368
location: https://synbiohub.org/public/igem/BBa_S04368/annotation2263287/range2263287/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04368/annotation2263287/range2263287/1
type: Range
persistent identity: range2263287
display id: range2263287
version: 1
top level: BBa_S04368
direction: direction/reverse
start: 1635
end: 1646
orientation: inline

BBa_S04366
type: ComponentDefinition
persistent identity: BBa S04366
display id: BBa_S04366
version: 1
was generated by: iGEM to SBOL conversion
description: S04365:C0078
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0078
component: BBa_S04365
sequence annotation: BBa_S04365
sequence annotation: BBa_C0078
sequence: https://synbiohub.org/public/igem/BBa_S04366_sequence/1

BBa_C0078
type: Component
persistent identity: component2256722
display id: component2256722
version: 1
top level: BBa_S04366
definition: lasI
access: public

BBa_S04365
type: Component
persistent identity: component2256718
display id: component2256718
version: 1
top level: BBa_S04366
definition: BBa_S04365
access: public

BBa_S04365
type: SequenceAnnotation
persistent identity: annotation2256718
display id: annotation2256718
version: 1
top level: BBa_S04366
location: https://synbiohub.org/public/igem/BBa_S04366/annotation2256718/range2256718/1
component: BBa_S04365

https://synbiohub.org/public/igem/BBa_S04366/annotation2256718/range2256718/1
type: Range
persistent identity: range2256718
display id: range2256718
version: 1
top level: BBa_S04366
direction: direction/reverse
start: 1
end: 953
orientation: inline

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation2256722
display id: annotation2256722
version: 1
top level: BBa_S04366
location: https://synbiohub.org/public/igem/BBa_S04366/annotation2256722/range2256722/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_S04366/annotation2256722/range2256722/1
type: Range
persistent identity: range2256722
display id: range2256722
version: 1
top level: BBa_S04366
direction: direction/reverse
start: 960
end: 1626
orientation: inline

lasI
type: ComponentDefinition
persistent identity: BBa C0078
display id: BBa_C0078
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for PAI from Pseudomonas aeruginosa
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for lasI protein, which produces the chemical signal AI-1
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Chris Walsh (Fighting Darwins)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: LasI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0078_sequence/1

LasI
type: SequenceAnnotation
persistent identity: annotation305970
display id: annotation305970
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1
type: Range
persistent identity: range305970
display id: range305970
version: 1
top level: lasI
direction: direction/forward
start: 1
end: 603
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306608
display id: annotation306608
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1
type: Range
persistent identity: range306608
display id: range306608
version: 1
top level: lasI
direction: direction/forward
start: 604
end: 636
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214003
display id: annotation2214003
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1
type: Range
persistent identity: range2214003
display id: range2214003
version: 1
top level: lasI
direction: direction/forward
start: 643
end: 667
orientation: inline

BBa_S04365
type: ComponentDefinition
persistent identity: BBa S04365
display id: BBa_S04365
version: 1
was generated by: iGEM to SBOL conversion
description: S04364:B0034
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_S04364
sequence annotation: BBa_S04364
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04365_sequence/1

BBa_B0034
type: Component
persistent identity: component2249990
display id: component2249990
version: 1
top level: BBa_S04365
definition: BBa_B0034
access: public

BBa_S04364
type: Component
persistent identity: component2249988
display id: component2249988
version: 1
top level: BBa_S04365
definition: BBa_S04364
access: public

BBa_S04364
type: SequenceAnnotation
persistent identity: annotation2249988
display id: annotation2249988
version: 1
top level: BBa_S04365
location: https://synbiohub.org/public/igem/BBa_S04365/annotation2249988/range2249988/1
component: BBa_S04364

https://synbiohub.org/public/igem/BBa_S04365/annotation2249988/range2249988/1
type: Range
persistent identity: range2249988
display id: range2249988
version: 1
top level: BBa_S04365
direction: direction/reverse
start: 1
end: 933
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2249990
display id: annotation2249990
version: 1
top level: BBa_S04365
location: https://synbiohub.org/public/igem/BBa_S04365/annotation2249990/range2249990/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04365/annotation2249990/range2249990/1
type: Range
persistent identity: range2249990
display id: range2249990
version: 1
top level: BBa_S04365
direction: direction/reverse
start: 942
end: 953
orientation: inline

BBa_S04364
type: ComponentDefinition
persistent identity: BBa S04364
display id: BBa_S04364
version: 1
was generated by: iGEM to SBOL conversion
description: S04363:C0079
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0079
component: BBa_S04363
sequence annotation: BBa_S04363
sequence annotation: BBa_C0079
sequence: https://synbiohub.org/public/igem/BBa_S04364_sequence/1

BBa_C0079
type: Component
persistent identity: component2246977
display id: component2246977
version: 1
top level: BBa_S04364
definition: lasr
access: public

BBa_S04363
type: Component
persistent identity: component2246972
display id: component2246972
version: 1
top level: BBa_S04364
definition: BBa_S04363
access: public

BBa_S04363
type: SequenceAnnotation
persistent identity: annotation2246972
display id: annotation2246972
version: 1
top level: BBa_S04364
location: https://synbiohub.org/public/igem/BBa_S04364/annotation2246972/range2246972/1
component: BBa_S04363

https://synbiohub.org/public/igem/BBa_S04364/annotation2246972/range2246972/1
type: Range
persistent identity: range2246972
display id: range2246972
version: 1
top level: BBa_S04364
direction: direction/reverse
start: 1
end: 146
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2246977
display id: annotation2246977
version: 1
top level: BBa_S04364
location: https://synbiohub.org/public/igem/BBa_S04364/annotation2246977/range2246977/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_S04364/annotation2246977/range2246977/1
type: Range
persistent identity: range2246977
display id: range2246977
version: 1
top level: BBa_S04364
direction: direction/reverse
start: 153
end: 933
orientation: inline

lasr
type: ComponentDefinition
persistent identity: BBa C0079
display id: BBa_C0079
version: 1
was generated by: iGEM to SBOL conversion
description: lasR activator from P. aeruginosa PAO1(+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: Genbank: NC_002516 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for lasR protein, which accepts chemical signal AI-1 (made by lasI protein)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Mutated 480 from g to a to remove PstI site
star: true
creator: Alvin Carter Powers (Fighting Darwins)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lasR
sequence annotation: G
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0079_sequence/1

lasR
type: SequenceAnnotation
persistent identity: annotation307935
display id: annotation307935
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1
type: Range
persistent identity: range307935
display id: range307935
version: 1
top level: lasr
direction: direction/forward
start: 1
end: 717
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation308006
display id: annotation308006
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1
type: Range
persistent identity: range308006
display id: range308006
version: 1
top level: lasr
direction: direction/forward
start: 480
end: 480
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation307948
display id: annotation307948
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1
type: Range
persistent identity: range307948
display id: range307948
version: 1
top level: lasr
direction: direction/forward
start: 718
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213999
display id: annotation2213999
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1
type: Range
persistent identity: range2213999
display id: range2213999
version: 1
top level: lasr
direction: direction/forward
start: 757
end: 781
orientation: inline

BBa_S04363
type: ComponentDefinition
persistent identity: BBa S04363
display id: BBa_S04363
version: 1
was generated by: iGEM to SBOL conversion
description: K091146:B0034
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_K091146
sequence annotation: BBa_K091146
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04363_sequence/1

BBa_B0034
type: Component
persistent identity: component2063465
display id: component2063465
version: 1
top level: BBa_S04363
definition: BBa_B0034
access: public

BBa_K091146
type: Component
persistent identity: component2063463
display id: component2063463
version: 1
top level: BBa_S04363
definition: BBa_K091146
access: public

BBa_K091146
type: SequenceAnnotation
persistent identity: annotation2063463
display id: annotation2063463
version: 1
top level: BBa_S04363
location: https://synbiohub.org/public/igem/BBa_S04363/annotation2063463/range2063463/1
component: BBa_K091146

https://synbiohub.org/public/igem/BBa_S04363/annotation2063463/range2063463/1
type: Range
persistent identity: range2063463
display id: range2063463
version: 1
top level: BBa_S04363
direction: direction/reverse
start: 1
end: 126
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2063465
display id: annotation2063465
version: 1
top level: BBa_S04363
location: https://synbiohub.org/public/igem/BBa_S04363/annotation2063465/range2063465/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04363/annotation2063465/range2063465/1
type: Range
persistent identity: range2063465
display id: range2063465
version: 1
top level: BBa_S04363
direction: direction/reverse
start: 135
end: 146
orientation: inline

BBa_K091146
type: ComponentDefinition
persistent identity: BBa K091146
display id: BBa_K091146
version: 1
was generated by: iGEM to SBOL conversion
description: pLas/Lux Hybrid Promoter
created: 2008-07-16T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: The LuxR binding site is from BBa_R0062 and the pLas' promoter is from the region upstream of the LasI gene in Pseudomonas aeruginosa.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: K091146 is a modified version of the pLas' promoter (BBa_K091117) containing the addition of a luxR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of PAI-1 and LasR and repressed in the presence of AI-1 and LuxR.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The Lux box was placed adjacent to the LasR binding site with the aim of minimizing cross-talk in the presence of both PAI-1 and AI-1; since the LasR site should have a higher affinity, we hope that the binding of PAI-1 will competitively exclude the binding of AI-1.
star: true
creator: Erin Feeney
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: LasR
sequence annotation: -35
sequence annotation: LuxR
sequence annotation: -10
sequence annotation: Transcriptional Start
sequence: https://synbiohub.org/public/igem/BBa_K091146_sequence/1

LasR
type: SequenceAnnotation
persistent identity: annotation1967354
display id: annotation1967354
version: 1
top level: BBa_K091146
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1
type: Range
persistent identity: range1967354
display id: range1967354
version: 1
top level: BBa_K091146
direction: direction/forward
start: 75
end: 93
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1967357
display id: annotation1967357
version: 1
top level: BBa_K091146
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1
type: Range
persistent identity: range1967357
display id: range1967357
version: 1
top level: BBa_K091146
direction: direction/forward
start: 86
end: 91
orientation: inline

LuxR
type: SequenceAnnotation
persistent identity: annotation1967355
display id: annotation1967355
version: 1
top level: BBa_K091146
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1
type: Range
persistent identity: range1967355
display id: range1967355
version: 1
top level: BBa_K091146
direction: direction/forward
start: 94
end: 113
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1967356
display id: annotation1967356
version: 1
top level: BBa_K091146
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1
type: Range
persistent identity: range1967356
display id: range1967356
version: 1
top level: BBa_K091146
direction: direction/forward
start: 113
end: 118
orientation: inline

Transcriptional Start
type: SequenceAnnotation
persistent identity: annotation1967358
display id: annotation1967358
version: 1
top level: BBa_K091146
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1
type: Range
persistent identity: range1967358
display id: range1967358
version: 1
top level: BBa_K091146
direction: direction/forward
start: 125
end: 125
orientation: inline

https://synbiohub.org/public/igem/BBa_S04371_sequence/1
type: Sequence
persistent identity: BBa S04371 sequence
display id: BBa_S04371_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04353_sequence/1
type: Sequence
persistent identity: BBa S04353 sequence
display id: BBa_S04353_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04370_sequence/1
type: Sequence
persistent identity: BBa S04370 sequence
display id: BBa_S04370_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04369_sequence/1
type: Sequence
persistent identity: BBa S04369 sequence
display id: BBa_S04369_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31006_sequence/1
type: Sequence
persistent identity: BBa J31006 sequence
display id: BBa_J31006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04368_sequence/1
type: Sequence
persistent identity: BBa S04368 sequence
display id: BBa_S04368_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04366_sequence/1
type: Sequence
persistent identity: BBa S04366 sequence
display id: BBa_S04366_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0078_sequence/1
type: Sequence
persistent identity: BBa C0078 sequence
display id: BBa_C0078_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04365_sequence/1
type: Sequence
persistent identity: BBa S04365 sequence
display id: BBa_S04365_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04364_sequence/1
type: Sequence
persistent identity: BBa S04364 sequence
display id: BBa_S04364_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0079_sequence/1
type: Sequence
persistent identity: BBa C0079 sequence
display id: BBa_C0079_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04363_sequence/1
type: Sequence
persistent identity: BBa S04363 sequence
display id: BBa_S04363_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091146_sequence/1
type: Sequence
persistent identity: BBa K091146 sequence
display id: BBa_K091146_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z