BBa_S04379
type: ComponentDefinition
persistent identity: BBa S04379
display id: BBa_S04379
version: 1
was generated by: iGEM to SBOL conversion
description: S04378:J31006
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J31006
component: BBa_S04378
sequence annotation: BBa_S04378
sequence annotation: BBa_J31006
sequence: https://synbiohub.org/public/igem/BBa_S04379_sequence/1

BBa_J31006
type: Component
persistent identity: component2268051
display id: component2268051
version: 1
top level: BBa_S04379
definition: tetA(C)b
access: public

BBa_S04378
type: Component
persistent identity: component2268046
display id: component2268046
version: 1
top level: BBa_S04379
definition: BBa_S04378
access: public

BBa_S04378
type: SequenceAnnotation
persistent identity: annotation2268046
display id: annotation2268046
version: 1
top level: BBa_S04379
location: https://synbiohub.org/public/igem/BBa_S04379/annotation2268046/range2268046/1
component: BBa_S04378

https://synbiohub.org/public/igem/BBa_S04379/annotation2268046/range2268046/1
type: Range
persistent identity: range2268046
display id: range2268046
version: 1
top level: BBa_S04379
direction: direction/reverse
start: 1
end: 1817
orientation: inline

BBa_J31006
type: SequenceAnnotation
persistent identity: annotation2268051
display id: annotation2268051
version: 1
top level: BBa_S04379
location: https://synbiohub.org/public/igem/BBa_S04379/annotation2268051/range2268051/1
component: BBa_J31006

https://synbiohub.org/public/igem/BBa_S04379/annotation2268051/range2268051/1
type: Range
persistent identity: range2268051
display id: range2268051
version: 1
top level: BBa_S04379
direction: direction/reverse
start: 1826
end: 3016
orientation: inline

tetA(C)b
type: ComponentDefinition
persistent identity: BBa J31006
display id: BBa_J31006
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This is the coding region for Tetracyline resistance in the reverse orientation. If a promoter adn RBS are placed downstream and the entire region, from promoter to stop codon is flipped the cell will express tetracycline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plasmids.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: stop
sequence annotation: tetA(C) backwards
sequence annotation: Rev primer J31006
sequence annotation: Fwd primer J31006
sequence: https://synbiohub.org/public/igem/BBa_J31006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1910574
display id: annotation1910574
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
type: Range
persistent identity: range1910574
display id: range1910574
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1
end: 3
orientation: inline

tetA(C) backwards
type: SequenceAnnotation
persistent identity: annotation1884998
display id: annotation1884998
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
type: Range
persistent identity: range1884998
display id: range1884998
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1
end: 1191
orientation: inline

Rev primer J31006
type: SequenceAnnotation
persistent identity: annotation1910575
display id: annotation1910575
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
type: Range
persistent identity: range1910575
display id: range1910575
version: 1
top level: tetA(C)b
direction: direction/forward
start: 1
end: 20
orientation: inline

Fwd primer J31006
type: SequenceAnnotation
persistent identity: annotation1910576
display id: annotation1910576
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
type: Range
persistent identity: range1910576
display id: range1910576
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1168
end: 1191
orientation: inline

BBa_S04378
type: ComponentDefinition
persistent identity: BBa S04378
display id: BBa_S04378
version: 1
was generated by: iGEM to SBOL conversion
description: S04377:B0034
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_S04377
sequence annotation: BBa_S04377
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04378_sequence/1

BBa_B0034
type: Component
persistent identity: component2263310
display id: component2263310
version: 1
top level: BBa_S04378
definition: BBa_B0034
access: public

BBa_S04377
type: Component
persistent identity: component2263308
display id: component2263308
version: 1
top level: BBa_S04378
definition: BBa_S04377
access: public

BBa_S04377
type: SequenceAnnotation
persistent identity: annotation2263308
display id: annotation2263308
version: 1
top level: BBa_S04378
location: https://synbiohub.org/public/igem/BBa_S04378/annotation2263308/range2263308/1
component: BBa_S04377

https://synbiohub.org/public/igem/BBa_S04378/annotation2263308/range2263308/1
type: Range
persistent identity: range2263308
display id: range2263308
version: 1
top level: BBa_S04378
direction: direction/reverse
start: 1
end: 1797
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2263310
display id: annotation2263310
version: 1
top level: BBa_S04378
location: https://synbiohub.org/public/igem/BBa_S04378/annotation2263310/range2263310/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04378/annotation2263310/range2263310/1
type: Range
persistent identity: range2263310
display id: range2263310
version: 1
top level: BBa_S04378
direction: direction/reverse
start: 1806
end: 1817
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_S04377
type: ComponentDefinition
persistent identity: BBa S04377
display id: BBa_S04377
version: 1
was generated by: iGEM to SBOL conversion
description: S04376:C0076
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0076
component: BBa_S04376
sequence annotation: BBa_S04376
sequence annotation: BBa_C0076
sequence: https://synbiohub.org/public/igem/BBa_S04377_sequence/1

BBa_C0076
type: Component
persistent identity: component2256762
display id: component2256762
version: 1
top level: BBa_S04377
definition: cinI
access: public

BBa_S04376
type: Component
persistent identity: component2256758
display id: component2256758
version: 1
top level: BBa_S04377
definition: BBa_S04376
access: public

BBa_S04376
type: SequenceAnnotation
persistent identity: annotation2256758
display id: annotation2256758
version: 1
top level: BBa_S04377
location: https://synbiohub.org/public/igem/BBa_S04377/annotation2256758/range2256758/1
component: BBa_S04376

https://synbiohub.org/public/igem/BBa_S04377/annotation2256758/range2256758/1
type: Range
persistent identity: range2256758
display id: range2256758
version: 1
top level: BBa_S04377
direction: direction/reverse
start: 1
end: 1064
orientation: inline

BBa_C0076
type: SequenceAnnotation
persistent identity: annotation2256762
display id: annotation2256762
version: 1
top level: BBa_S04377
location: https://synbiohub.org/public/igem/BBa_S04377/annotation2256762/range2256762/1
component: BBa_C0076

https://synbiohub.org/public/igem/BBa_S04377/annotation2256762/range2256762/1
type: Range
persistent identity: range2256762
display id: range2256762
version: 1
top level: BBa_S04377
direction: direction/reverse
start: 1071
end: 1797
orientation: inline

cinI
type: ComponentDefinition
persistent identity: BBa C0076
display id: BBa_C0076
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: cinI codes for an autoinducer synthetase which utilizes SAM to make O3-C14:1-HSL. In complex with O3-C14:1-HSL, CinR (BBa_C0077) binds to the Cin promoter (BBa_R0077) and activates transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tga -> tAaTAA
star: true
creator: crackdots
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: CinI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0076_sequence/1

CinI
type: SequenceAnnotation
persistent identity: annotation301035
display id: annotation301035
version: 1
top level: cinI
location: https://synbiohub.org/public/igem/BBa_C0076/annotation301035/range301035/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0076/annotation301035/range301035/1
type: Range
persistent identity: range301035
display id: range301035
version: 1
top level: cinI
direction: direction/forward
start: 1
end: 663
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306586
display id: annotation306586
version: 1
top level: cinI
location: https://synbiohub.org/public/igem/BBa_C0076/annotation306586/range306586/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0076/annotation306586/range306586/1
type: Range
persistent identity: range306586
display id: range306586
version: 1
top level: cinI
direction: direction/forward
start: 664
end: 696
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214001
display id: annotation2214001
version: 1
top level: cinI
location: https://synbiohub.org/public/igem/BBa_C0076/annotation2214001/range2214001/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0076/annotation2214001/range2214001/1
type: Range
persistent identity: range2214001
display id: range2214001
version: 1
top level: cinI
direction: direction/forward
start: 703
end: 727
orientation: inline

BBa_S04376
type: ComponentDefinition
persistent identity: BBa S04376
display id: BBa_S04376
version: 1
was generated by: iGEM to SBOL conversion
description: S04375:B0034
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_S04375
sequence annotation: BBa_S04375
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04376_sequence/1

BBa_B0034
type: Component
persistent identity: component2250005
display id: component2250005
version: 1
top level: BBa_S04376
definition: BBa_B0034
access: public

BBa_S04375
type: Component
persistent identity: component2250003
display id: component2250003
version: 1
top level: BBa_S04376
definition: BBa_S04375
access: public

BBa_S04375
type: SequenceAnnotation
persistent identity: annotation2250003
display id: annotation2250003
version: 1
top level: BBa_S04376
location: https://synbiohub.org/public/igem/BBa_S04376/annotation2250003/range2250003/1
component: BBa_S04375

https://synbiohub.org/public/igem/BBa_S04376/annotation2250003/range2250003/1
type: Range
persistent identity: range2250003
display id: range2250003
version: 1
top level: BBa_S04376
direction: direction/reverse
start: 1
end: 1044
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2250005
display id: annotation2250005
version: 1
top level: BBa_S04376
location: https://synbiohub.org/public/igem/BBa_S04376/annotation2250005/range2250005/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04376/annotation2250005/range2250005/1
type: Range
persistent identity: range2250005
display id: range2250005
version: 1
top level: BBa_S04376
direction: direction/reverse
start: 1053
end: 1064
orientation: inline

BBa_S04375
type: ComponentDefinition
persistent identity: BBa S04375
display id: BBa_S04375
version: 1
was generated by: iGEM to SBOL conversion
description: S04374:C0077
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0077
component: BBa_S04374
sequence annotation: BBa_S04374
sequence annotation: BBa_C0077
sequence: https://synbiohub.org/public/igem/BBa_S04375_sequence/1

BBa_C0077
type: Component
persistent identity: component2246989
display id: component2246989
version: 1
top level: BBa_S04375
definition: cinr
access: public

BBa_S04374
type: Component
persistent identity: component2246985
display id: component2246985
version: 1
top level: BBa_S04375
definition: BBa_S04374
access: public

BBa_S04374
type: SequenceAnnotation
persistent identity: annotation2246985
display id: annotation2246985
version: 1
top level: BBa_S04375
location: https://synbiohub.org/public/igem/BBa_S04375/annotation2246985/range2246985/1
component: BBa_S04374

https://synbiohub.org/public/igem/BBa_S04375/annotation2246985/range2246985/1
type: Range
persistent identity: range2246985
display id: range2246985
version: 1
top level: BBa_S04375
direction: direction/reverse
start: 1
end: 251
orientation: inline

BBa_C0077
type: SequenceAnnotation
persistent identity: annotation2246989
display id: annotation2246989
version: 1
top level: BBa_S04375
location: https://synbiohub.org/public/igem/BBa_S04375/annotation2246989/range2246989/1
component: BBa_C0077

https://synbiohub.org/public/igem/BBa_S04375/annotation2246989/range2246989/1
type: Range
persistent identity: range2246989
display id: range2246989
version: 1
top level: BBa_S04375
direction: direction/reverse
start: 258
end: 1044
orientation: inline

cinr
type: ComponentDefinition
persistent identity: BBa C0077
display id: BBa_C0077
version: 1
was generated by: iGEM to SBOL conversion
description: cinR activator from Rhizobium leguminosarum (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with O3-C14:1-HSL (made by BBa_C0076), CinR binds to the Cin promoter (BBa_R0077) and activates transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tag -> taATAA
star: true
creator: crackdots
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cinR
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0077_sequence/1

cinR
type: SequenceAnnotation
persistent identity: annotation301112
display id: annotation301112
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1
type: Range
persistent identity: range301112
display id: range301112
version: 1
top level: cinr
direction: direction/forward
start: 1
end: 723
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306600
display id: annotation306600
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1
type: Range
persistent identity: range306600
display id: range306600
version: 1
top level: cinr
direction: direction/forward
start: 724
end: 756
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214000
display id: annotation2214000
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1
type: Range
persistent identity: range2214000
display id: range2214000
version: 1
top level: cinr
direction: direction/forward
start: 763
end: 787
orientation: inline

BBa_S04374
type: ComponentDefinition
persistent identity: BBa S04374
display id: BBa_S04374
version: 1
was generated by: iGEM to SBOL conversion
description: R0077:B0034
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_R0077
sequence annotation: BBa_R0077
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04374_sequence/1

BBa_B0034
type: Component
persistent identity: component2063713
display id: component2063713
version: 1
top level: BBa_S04374
definition: BBa_B0034
access: public

BBa_R0077
type: Component
persistent identity: component2063711
display id: component2063711
version: 1
top level: BBa_S04374
definition: cinR
access: public

BBa_R0077
type: SequenceAnnotation
persistent identity: annotation2063711
display id: annotation2063711
version: 1
top level: BBa_S04374
location: https://synbiohub.org/public/igem/BBa_S04374/annotation2063711/range2063711/1
component: BBa_R0077

https://synbiohub.org/public/igem/BBa_S04374/annotation2063711/range2063711/1
type: Range
persistent identity: range2063711
display id: range2063711
version: 1
top level: BBa_S04374
direction: direction/reverse
start: 1
end: 231
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2063713
display id: annotation2063713
version: 1
top level: BBa_S04374
location: https://synbiohub.org/public/igem/BBa_S04374/annotation2063713/range2063713/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04374/annotation2063713/range2063713/1
type: Range
persistent identity: range2063713
display id: range2063713
version: 1
top level: BBa_S04374
direction: direction/reverse
start: 240
end: 251
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa R0077
display id: BBa_R0077
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (cinR and HSL regulated, RBS+)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: CinR (BBa_C0077) in complex with O3-C14:1-HSL (from CinI, BBa_C0076) binds to this promoter and activates transcription. This promoter contains the RBS: TGGAGG
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: the last 5 bases (gctaa) removed to allow for the correct spacing between the RBS (TGGAGG) and the start of the downstream gene after biobricks splicing.
star: true
creator: crackdots
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: unidentified/uncharacterized CinR dependent
sequence annotation: stem_loop
sequence annotation: embedded RBS
sequence: https://synbiohub.org/public/igem/BBa_R0077_sequence/1

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation301371
display id: annotation301371
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301371/range301371/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0077/annotation301371/range301371/1
type: Range
persistent identity: range301371
display id: range301371
version: 1
top level: cinR
direction: direction/forward
start: 1
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation301204
display id: annotation301204
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301204/range301204/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_R0077/annotation301204/range301204/1
type: Range
persistent identity: range301204
display id: range301204
version: 1
top level: cinR
direction: direction/forward
start: 35
end: 74
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation301205
display id: annotation301205
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301205/range301205/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_R0077/annotation301205/range301205/1
type: Range
persistent identity: range301205
display id: range301205
version: 1
top level: cinR
direction: direction/forward
start: 91
end: 136
orientation: inline

embedded RBS
type: SequenceAnnotation
persistent identity: annotation301150
display id: annotation301150
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301150/range301150/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_R0077/annotation301150/range301150/1
type: Range
persistent identity: range301150
display id: range301150
version: 1
top level: cinR
direction: direction/forward
start: 226
end: 231
orientation: inline

https://synbiohub.org/public/igem/BBa_S04379_sequence/1
type: Sequence
persistent identity: BBa S04379 sequence
display id: BBa_S04379_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctggaggtactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgatagtgctagtgtagatccctactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31006_sequence/1
type: Sequence
persistent identity: BBa J31006 sequence
display id: BBa_J31006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04378_sequence/1
type: Sequence
persistent identity: BBa S04378 sequence
display id: BBa_S04378_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctggaggtactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgatagtgctagtgtagatccctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04377_sequence/1
type: Sequence
persistent identity: BBa S04377 sequence
display id: BBa_S04377_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctggaggtactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0076_sequence/1
type: Sequence
persistent identity: BBa C0076 sequence
display id: BBa_C0076_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04376_sequence/1
type: Sequence
persistent identity: BBa S04376 sequence
display id: BBa_S04376_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctggaggtactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04375_sequence/1
type: Sequence
persistent identity: BBa S04375 sequence
display id: BBa_S04375_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctggaggtactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0077_sequence/1
type: Sequence
persistent identity: BBa C0077 sequence
display id: BBa_C0077_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04374_sequence/1
type: Sequence
persistent identity: BBa S04374 sequence
display id: BBa_S04374_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctggaggtactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0077_sequence/1
type: Sequence
persistent identity: BBa R0077 sequence
display id: BBa_R0077_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctggagg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z