LOCUS       BBa_S04380              3036 bp    DNA     linear       15-Jul-2024
DEFINITION  S04379:B0034
ACCESSION   BBa_S04380
VERSION     BBa_S04380.1  
FEATURES             Location/Qualifiers
     RBS             226..231
                     /label=embedded RBS
     stem_loop       35..74
                     /label=stem_loop
     stem_loop       91..136
                     /label=stem_loop
     promoter        1..220
                     /label=unidentified/uncharacterized CinR dependent
     promoter        1..231
                     /label=BBa_R0077
     misc_feature    244..247
                     /label=conserved
     RBS             240..251
                     /label=BBa_B0034
     misc_feature    1..251
                     /label=BBa_S04374
     CDS             258..980
                     /label=cinR
     misc_feature    981..1013
                     /label=LVA
     misc_feature    1020..1044
                     /label=Help:Barcodes
     CDS             258..1044
                     /label=BBa_C0077
     misc_feature    1..1044
                     /label=BBa_S04375
     misc_feature    1057..1060
                     /label=conserved
     RBS             1053..1064
                     /label=BBa_B0034
     misc_feature    1..1064
                     /label=BBa_S04376
     CDS             1071..1733
                     /label=CinI
     misc_feature    1734..1766
                     /label=LVA
     misc_feature    1773..1797
                     /label=Help:Barcodes
     CDS             1071..1797
                     /label=BBa_C0076
     misc_feature    1..1797
                     /label=BBa_S04377
     misc_feature    1810..1813
                     /label=conserved
     RBS             1806..1817
                     /label=BBa_B0034
     misc_feature    1..1817
                     /label=BBa_S04378
     CDS             1826..3016
                     /label=tetA(C) backwards
     misc_feature    1826..1828
                     /label=stop
     primer_bind     1826..1845
                     /label=Rev primer J31006
     primer_bind     2993..3016
                     /label=Fwd primer J31006
     CDS             1826..3016
                     /label=BBa_J31006
     misc_feature    1..3016
                     /label=BBa_S04379
     misc_feature    3029..3032
                     /label=conserved
     RBS             3025..3036
                     /label=BBa_B0034
ORIGIN
        1 ccctttgtgc gtccaaacgg acgcacggcg ctctaaagcg ggtcgcgatc tttcagattc
       61 gctcctcgcg ctttcagtct ttgttttggc gcatgtcgtt atcgcaaaac cgctgcacac
      121 ttttgcgcga catgctctga tccccctcat ctgggggggc ctatctgagg gaatttccga
      181 tccggctcgc ctgaaccatt ctgctttcca cgaacttgaa aacgctggag gtactagaga
      241 aagaggagaa atactagatg attgagaata cctatagcga aaagttcgag tccgcgttcg
      301 aacagatcaa ggcggcggcc aacgtggatg ccgccatccg tattctccag gcggaatata
      361 acctcgattt cgtcacctac catctcgccc agacgatcgc gagcaagatc gattcgccct
      421 tcgtgcgcac cacctatccg gatgcctggg tttcccgcta cctcctcaac agctatgtga
      481 aggtcgatcc gatcgtcaag cagggcttcg aacgccagct gcccttcgac tggagcgagg
      541 tcgaaccgac gccggaggcc tatgccatgc tggtcgacgc ccagaaacac ggcatcggtg
      601 gcaatggcta ctccatcccc gtcgccgaca aggcgcagcg ccgcgccctg ctgtcgctga
      661 atgcccgtat accggccgac gaatggaccg agctcgtgcg ccgctgccgc aacgagtgga
      721 tcgagatcgc ccatctgatc caccgcaagg ccgtctatga gctgcatggc gaaaacgatc
      781 cggtgccggc attgtcgccg cgcgagatcg agtgtctgca ctggaccgcc ctcggcaagg
      841 attacaagga tatttcggtc atcctgggca tatcagagca taccacacgc gattacctga
      901 agaccgcccg cttcaagctc ggctgcgcca cgatctcggc cgccgcgtcg cgggctgttc
      961 aattgcgcat catcaatccc gctgcaaacg acgaaaacta cgctttagta gcttaataac
     1021 gctgatagtg ctagtgtaga tcgctactag agaaagagga gaaatactag atgttcgtta
     1081 tcatccaggc tcacgaatac cagaaatacg ctgctgttct ggaccagatg ttccgtctgc
     1141 gtaaaaaagt tttcgctgac accctgtgct gggacgttcc ggttatcggt ccgtacgaac
     1201 gtgactccta cgactccctg gctccggctt acctggtttg gtgcaacgac tcccgtaccc
     1261 gtctgtacgg tggtatgcgt ctgatgccga ccaccggtcc gaccctgctg tacgacgttt
     1321 tccgtgaaac cttcccggac gctgctgacc tgatcgctcc gggtatctgg gaaggtaccc
     1381 gtatgtgcat cgacgaagaa gctatcgcta aagacttccc ggaaatcgac gctggtcgtg
     1441 ctttctccat gatgctgctg gctctgtgcg aatgcgctct ggaccacggt atccacacca
     1501 tgatctccaa ctacgaaccg tacctgaaac gtgtttacaa acgtgctggt gctgaagttg
     1561 aagaactggg tcgtgctgac ggttacggta aatacccggt ttgctgcggt gctttcgaag
     1621 tttccgaccg tgttctgcgt aaaatgcgtg ctgctctggg tctgaccctg ccgctgtacg
     1681 ttcgtcacgt tccggctcgt tccgttgtta cccagttcct ggaaatggct gctgctgcta
     1741 acgacgaaaa ctacgctctg gttgcttaat aaccctgata gtgctagtgt agatccctac
     1801 tagagaaaga ggagaaatac tagagttagg tcgaggtggc ccggctccat gcaccgcgac
     1861 gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc
     1921 atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccaatg atcgaagtta
     1981 ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc
     2041 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa
     2101 tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg
     2161 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg gggccagtga
     2221 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg
     2281 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc
     2341 ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg
     2401 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct
     2461 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
     2521 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
     2581 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     2641 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     2701 ccacgatgcg tccggcgtag aggatccaca ggacgggtgt ggtcgccatg atcgcgtagt
     2761 cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca
     2821 gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc
     2881 catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg
     2941 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
     3001 cattgttaga tttcattact agagaaagag gagaaa
//