##gff-version 3
##sequence-region BBa_S04381 1 3765
BBa_S04381	.	engineered_region	1	3036	.	+	0	ID=BBa_S04380;Name=BBa_S04380
BBa_S04381	.	engineered_region	1	3016	.	+	0	ID=BBa_S04379;Name=BBa_S04379;Parent=BBa_S04380
BBa_S04381	.	engineered_region	1	1817	.	+	0	ID=BBa_S04378;Name=BBa_S04378;Parent=BBa_S04379
BBa_S04381	.	engineered_region	1	1797	.	+	0	ID=BBa_S04377;Name=BBa_S04377;Parent=BBa_S04378
BBa_S04381	.	engineered_region	1	1064	.	+	0	ID=BBa_S04376;Name=BBa_S04376;Parent=BBa_S04377
BBa_S04381	.	ribosome_entry_site	1053	1064	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04376
BBa_S04381	.	conserved_region	1057	1060	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04381	.	engineered_region	1	1044	.	+	0	ID=BBa_S04375;Name=BBa_S04375;Parent=BBa_S04376
BBa_S04381	.	engineered_region	1	251	.	+	0	ID=BBa_S04374;Name=BBa_S04374;Parent=BBa_S04375
BBa_S04381	.	ribosome_entry_site	240	251	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04374
BBa_S04381	.	conserved_region	244	247	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04381	.	promoter	1	231	.	+	0	ID=BBa_R0077;Name=BBa_R0077;Parent=BBa_S04374
BBa_S04381	.	ribosome_entry_site	226	231	.	+	0	ID=annotation301150;Name=embedded RBS;Parent=BBa_R0077
BBa_S04381	.	stem_loop	91	136	.	+	0	ID=annotation301205;Name=stem_loop;Parent=BBa_R0077
BBa_S04381	.	promoter	1	220	.	+	0	ID=annotation301371;Name=unidentified/uncharacterized CinR dependent;Parent=BBa_R0077
BBa_S04381	.	stem_loop	35	74	.	+	0	ID=annotation301204;Name=stem_loop;Parent=BBa_R0077
BBa_S04381	.	CDS	258	1044	.	+	0	ID=BBa_C0077;Name=BBa_C0077;Parent=BBa_S04375
BBa_S04381	.	engineered_tag	1020	1044	.	+	0	ID=annotation2214000;Name=Help:Barcodes;Parent=BBa_C0077
BBa_S04381	.	tag	981	1013	.	+	0	ID=annotation306600;Name=LVA;Parent=BBa_C0077
BBa_S04381	.	CDS	258	980	.	+	0	ID=annotation301112;Name=cinR;Parent=BBa_C0077
BBa_S04381	.	CDS	1071	1797	.	+	0	ID=BBa_C0076;Name=BBa_C0076;Parent=BBa_S04377
BBa_S04381	.	CDS	1071	1733	.	+	0	ID=annotation301035;Name=CinI;Parent=BBa_C0076
BBa_S04381	.	tag	1734	1766	.	+	0	ID=annotation306586;Name=LVA;Parent=BBa_C0076
BBa_S04381	.	engineered_tag	1773	1797	.	+	0	ID=annotation2214001;Name=Help:Barcodes;Parent=BBa_C0076
BBa_S04381	.	ribosome_entry_site	1806	1817	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04378
BBa_S04381	.	conserved_region	1810	1813	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04381	.	CDS	1826	3016	.	+	0	ID=BBa_J31006;Name=BBa_J31006;Parent=BBa_S04379
BBa_S04381	.	primer_binding_site	1826	1845	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_S04381	.	stop_codon	1826	1828	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S04381	.	primer_binding_site	2993	3016	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_S04381	.	CDS	1826	3016	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S04381	.	ribosome_entry_site	3025	3036	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04380
BBa_S04381	.	conserved_region	3029	3032	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04381	.	CDS	3043	3765	.	+	0	ID=BBa_E0020;Name=BBa_E0020
>BBa_S04381
ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtc
tttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggg
gcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctggaggtactag
agaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggc
ggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacg
atcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagct
atgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgcc
ggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaag
gcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgca
acgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggc
attgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggc
atatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgt
cgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgata
gtgctagtgtagatcgctactagagaaagaggagaaatactagatgttcgttatcatccaggctcacgaataccagaaa
tacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggtta
tcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtct
gtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgct
gctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccgg
aaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccat
gatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgac
ggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgg
gtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgc
taacgacgaaaactacgctctggttgcttaataaccctgatagtgctagtgtagatccctactagagaaagaggagaaa
tactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgc
ctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcga
agttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcc
tgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacg
ccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggg
gccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccag
cgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtca
taagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcg
acgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaagg
aatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgc
tcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggc
gccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgata
gtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcata
gaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaa
gaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgca
ttgttagatttcattactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtg
cccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacct
acggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgac
ctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggc
tacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgaca
ccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaa
ctacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaac
atcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccg
acaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagtt
cgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa