LOCUS BBa_S04382 3902 bp DNA linear 05-Nov-2024 DEFINITION S04381:S04353 ACCESSION BBa_S04382 VERSION BBa_S04382.1 FEATURES Location/Qualifiers RBS 226..231 /label=embedded RBS stem_loop 35..74 /label=stem_loop stem_loop 91..136 /label=stem_loop promoter 1..220 /label=unidentified/uncharacterized CinR dependent promoter 1..231 /label=BBa_R0077 misc_feature 244..247 /label=conserved RBS 240..251 /label=BBa_B0034 misc_feature 1..251 /label=BBa_S04374 CDS 258..980 /label=cinR misc_feature 981..1013 /label=LVA misc_feature 1020..1044 /label=Help:Barcodes CDS 258..1044 /label=BBa_C0077 misc_feature 1..1044 /label=BBa_S04375 misc_feature 1057..1060 /label=conserved RBS 1053..1064 /label=BBa_B0034 misc_feature 1..1064 /label=BBa_S04376 CDS 1071..1733 /label=CinI misc_feature 1734..1766 /label=LVA misc_feature 1773..1797 /label=Help:Barcodes CDS 1071..1797 /label=BBa_C0076 misc_feature 1..1797 /label=BBa_S04377 misc_feature 1810..1813 /label=conserved RBS 1806..1817 /label=BBa_B0034 misc_feature 1..1817 /label=BBa_S04378 CDS 1826..3016 /label=tetA(C) backwards misc_feature 1826..1828 /label=stop primer_bind 1826..1845 /label=Rev primer J31006 primer_bind 2993..3016 /label=Fwd primer J31006 CDS 1826..3016 /label=BBa_J31006 misc_feature 1..3016 /label=BBa_S04379 misc_feature 3029..3032 /label=conserved RBS 3025..3036 /label=BBa_B0034 misc_feature 1..3036 /label=BBa_S04380 CDS 3043..3765 /label=BBa_E0020 misc_feature 1..3765 /label=BBa_S04381 stem_loop 3785..3828 /label=stem_loop misc_feature 3774..3853 /label=BBa_B0010 terminator 3774..3853 /label=BBa_B0010 stem_loop 3869..3888 /label=T7 TE misc_feature 3895..3895 /label=stop polyA_site 3889..3902 /label=polya misc_feature 3862..3902 /label=BBa_B0012 terminator 3862..3902 /label=BBa_B0012 misc_feature 3774..3902 /label=BBa_S04353 ORIGIN 1 ccctttgtgc gtccaaacgg acgcacggcg ctctaaagcg ggtcgcgatc tttcagattc 61 gctcctcgcg ctttcagtct ttgttttggc gcatgtcgtt atcgcaaaac cgctgcacac 121 ttttgcgcga catgctctga tccccctcat ctgggggggc ctatctgagg gaatttccga 181 tccggctcgc ctgaaccatt ctgctttcca cgaacttgaa aacgctggag gtactagaga 241 aagaggagaa atactagatg attgagaata cctatagcga aaagttcgag tccgcgttcg 301 aacagatcaa ggcggcggcc aacgtggatg ccgccatccg tattctccag gcggaatata 361 acctcgattt cgtcacctac catctcgccc agacgatcgc gagcaagatc gattcgccct 421 tcgtgcgcac cacctatccg gatgcctggg tttcccgcta cctcctcaac agctatgtga 481 aggtcgatcc gatcgtcaag cagggcttcg aacgccagct gcccttcgac tggagcgagg 541 tcgaaccgac gccggaggcc tatgccatgc tggtcgacgc ccagaaacac ggcatcggtg 601 gcaatggcta ctccatcccc gtcgccgaca aggcgcagcg ccgcgccctg ctgtcgctga 661 atgcccgtat accggccgac gaatggaccg agctcgtgcg ccgctgccgc aacgagtgga 721 tcgagatcgc ccatctgatc caccgcaagg ccgtctatga gctgcatggc gaaaacgatc 781 cggtgccggc attgtcgccg cgcgagatcg agtgtctgca ctggaccgcc ctcggcaagg 841 attacaagga tatttcggtc atcctgggca tatcagagca taccacacgc gattacctga 901 agaccgcccg cttcaagctc ggctgcgcca cgatctcggc cgccgcgtcg cgggctgttc 961 aattgcgcat catcaatccc gctgcaaacg acgaaaacta cgctttagta gcttaataac 1021 gctgatagtg ctagtgtaga tcgctactag agaaagagga gaaatactag atgttcgtta 1081 tcatccaggc tcacgaatac cagaaatacg ctgctgttct ggaccagatg ttccgtctgc 1141 gtaaaaaagt tttcgctgac accctgtgct gggacgttcc ggttatcggt ccgtacgaac 1201 gtgactccta cgactccctg gctccggctt acctggtttg gtgcaacgac tcccgtaccc 1261 gtctgtacgg tggtatgcgt ctgatgccga ccaccggtcc gaccctgctg tacgacgttt 1321 tccgtgaaac cttcccggac gctgctgacc tgatcgctcc gggtatctgg gaaggtaccc 1381 gtatgtgcat cgacgaagaa gctatcgcta aagacttccc ggaaatcgac gctggtcgtg 1441 ctttctccat gatgctgctg gctctgtgcg aatgcgctct ggaccacggt atccacacca 1501 tgatctccaa ctacgaaccg tacctgaaac gtgtttacaa acgtgctggt gctgaagttg 1561 aagaactggg tcgtgctgac ggttacggta aatacccggt ttgctgcggt gctttcgaag 1621 tttccgaccg tgttctgcgt aaaatgcgtg ctgctctggg tctgaccctg ccgctgtacg 1681 ttcgtcacgt tccggctcgt tccgttgtta cccagttcct ggaaatggct gctgctgcta 1741 acgacgaaaa ctacgctctg gttgcttaat aaccctgata gtgctagtgt agatccctac 1801 tagagaaaga ggagaaatac tagagttagg tcgaggtggc ccggctccat gcaccgcgac 1861 gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc 1921 atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccaatg atcgaagtta 1981 ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc 2041 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa 2101 tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg 2161 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg gggccagtga 2221 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg 2281 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc 2341 ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg 2401 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct 2461 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg 2521 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg 2581 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc 2641 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg 2701 ccacgatgcg tccggcgtag aggatccaca ggacgggtgt ggtcgccatg atcgcgtagt 2761 cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca 2821 gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc 2881 catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg 2941 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg 3001 cattgttaga tttcattact agagaaagag gagaaatact agatggtgag caagggcgag 3061 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt gaacggccac 3121 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 3181 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc 3241 tggggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag 3301 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 3361 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 3421 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac 3481 atcagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc 3541 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac 3601 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc 3661 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 3721 gccgccggga tcactctcgg catggacgag ctgtacaagt aataatacta gagccaggca 3781 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 3841 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 3901 ta // LOCUS BBa_B0015 129 bp DNA linear 05-Nov-2024 DEFINITION double terminator (B0010-B0012) ACCESSION BBa_B0015 VERSION BBa_B0015.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttata //