##gff-version 3
##sequence-region BBa_S04389 1 2911
BBa_S04389	.	engineered_region	1	1712	.	+	0	ID=BBa_S04388;Name=BBa_S04388
BBa_S04389	.	engineered_region	1	1692	.	+	0	ID=BBa_S04387;Name=BBa_S04387;Parent=BBa_S04388
BBa_S04389	.	CDS	966	1692	.	+	0	ID=BBa_C0076;Name=BBa_C0076;Parent=BBa_S04387
BBa_S04389	.	engineered_tag	1668	1692	.	+	0	ID=annotation2214001;Name=Help:Barcodes;Parent=BBa_C0076
BBa_S04389	.	CDS	966	1628	.	+	0	ID=annotation301035;Name=CinI;Parent=BBa_C0076
BBa_S04389	.	tag	1629	1661	.	+	0	ID=annotation306586;Name=LVA;Parent=BBa_C0076
BBa_S04389	.	engineered_region	1	959	.	+	0	ID=BBa_S04386;Name=BBa_S04386;Parent=BBa_S04387
BBa_S04389	.	ribosome_entry_site	948	959	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04386
BBa_S04389	.	conserved_region	952	955	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04389	.	engineered_region	1	939	.	+	0	ID=BBa_S04385;Name=BBa_S04385;Parent=BBa_S04386
BBa_S04389	.	CDS	153	939	.	+	0	ID=BBa_C0077;Name=BBa_C0077;Parent=BBa_S04385
BBa_S04389	.	engineered_tag	915	939	.	+	0	ID=annotation2214000;Name=Help:Barcodes;Parent=BBa_C0077
BBa_S04389	.	CDS	153	875	.	+	0	ID=annotation301112;Name=cinR;Parent=BBa_C0077
BBa_S04389	.	tag	876	908	.	+	0	ID=annotation306600;Name=LVA;Parent=BBa_C0077
BBa_S04389	.	engineered_region	1	146	.	+	0	ID=BBa_S04384;Name=BBa_S04384;Parent=BBa_S04385
BBa_S04389	.	ribosome_entry_site	135	146	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04384
BBa_S04389	.	conserved_region	139	142	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04389	.	promoter	1	126	.	+	0	ID=BBa_K091146;Name=BBa_K091146;Parent=BBa_S04384
BBa_S04389	.	sequence_feature	86	91	.	+	0	ID=annotation1967357;Name=-35;Parent=BBa_K091146
BBa_S04389	.	sequence_feature	113	118	.	+	0	ID=annotation1967356;Name=-10;Parent=BBa_K091146
BBa_S04389	.	non_covalent_binding_site	75	93	.	+	0	ID=annotation1967354;Name=LasR;Parent=BBa_K091146
BBa_S04389	.	start_codon	125	125	.	+	0	ID=annotation1967358;Name=Transcriptional Start;Parent=BBa_K091146
BBa_S04389	.	non_covalent_binding_site	94	113	.	+	0	ID=annotation1967355;Name=LuxR;Parent=BBa_K091146
BBa_S04389	.	ribosome_entry_site	1701	1712	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04388
BBa_S04389	.	conserved_region	1705	1708	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04389	.	CDS	1721	2911	.	+	0	ID=BBa_J31006;Name=BBa_J31006
BBa_S04389	.	primer_binding_site	1721	1740	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_S04389	.	CDS	1721	2911	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_S04389	.	stop_codon	1721	1723	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_S04389	.	primer_binding_site	2888	2911	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
>BBa_S04389
tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatcta
tctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatgatt
gagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgta
ttctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgccctt
cgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaag
cagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacg
cccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgct
gaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctg
atccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtc
tgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgatta
cctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatc
aatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagaga
aagaggagaaatactagatgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgtt
ccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctac
gactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccga
ccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctg
ggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctcc
atgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctga
aacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctg
cggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgt
cacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttg
cttaataaccctgatagtgctagtgtagatccctactagagaaagaggagaaatactagagttaggtcgaggtggcccg
gctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccat
gtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcg
atccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgcc
ggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcg
gccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgaggg
cgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgac
ccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccc
cgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgca
ttaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcc
caacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccga
tcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtc
cggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcag
gactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgct
agcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataacca
agcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcat