LOCUS BBa_S04391 3660 bp DNA linear 27-Dec-2024 DEFINITION S04390:E0020 ACCESSION BBa_S04391 VERSION BBa_S04391.1 FEATURES Location/Qualifiers misc_feature 75..93 /label=LasR misc_feature 94..113 /label=LuxR misc_feature 113..118 /label=-10 misc_feature 86..91 /label=-35 misc_feature 125..125 /label=Transcriptional Start promoter 1..126 /label=BBa_K091146 misc_feature 139..142 /label=conserved RBS 135..146 /label=BBa_B0034 misc_feature 1..146 /label=BBa_S04384 CDS 153..875 /label=cinR misc_feature 876..908 /label=LVA misc_feature 915..939 /label=Help:Barcodes CDS 153..939 /label=BBa_C0077 misc_feature 1..939 /label=BBa_S04385 misc_feature 952..955 /label=conserved RBS 948..959 /label=BBa_B0034 misc_feature 1..959 /label=BBa_S04386 CDS 966..1628 /label=CinI misc_feature 1629..1661 /label=LVA misc_feature 1668..1692 /label=Help:Barcodes CDS 966..1692 /label=BBa_C0076 misc_feature 1..1692 /label=BBa_S04387 misc_feature 1705..1708 /label=conserved RBS 1701..1712 /label=BBa_B0034 misc_feature 1..1712 /label=BBa_S04388 CDS 1721..2911 /label=tetA(C) backwards misc_feature 1721..1723 /label=stop primer_bind 1721..1740 /label=Rev primer J31006 primer_bind 2888..2911 /label=Fwd primer J31006 CDS 1721..2911 /label=BBa_J31006 misc_feature 1..2911 /label=BBa_S04389 misc_feature 2924..2927 /label=conserved RBS 2920..2931 /label=BBa_B0034 misc_feature 1..2931 /label=BBa_S04390 CDS 2938..3660 /label=BBa_E0020 ORIGIN 1 tgttctcgtg tgaagccatt gctctgatct tttggacgtt tcttcgagcc tagcaagggt 61 ccgggttcac cgaaatctat ctcatttgct agtacctgta ggatcgtaca ggtataaatt 121 cttcagtact agagaaagag gagaaatact agatgattga gaatacctat agcgaaaagt 181 tcgagtccgc gttcgaacag atcaaggcgg cggccaacgt ggatgccgcc atccgtattc 241 tccaggcgga atataacctc gatttcgtca cctaccatct cgcccagacg atcgcgagca 301 agatcgattc gcccttcgtg cgcaccacct atccggatgc ctgggtttcc cgctacctcc 361 tcaacagcta tgtgaaggtc gatccgatcg tcaagcaggg cttcgaacgc cagctgccct 421 tcgactggag cgaggtcgaa ccgacgccgg aggcctatgc catgctggtc gacgcccaga 481 aacacggcat cggtggcaat ggctactcca tccccgtcgc cgacaaggcg cagcgccgcg 541 ccctgctgtc gctgaatgcc cgtataccgg ccgacgaatg gaccgagctc gtgcgccgct 601 gccgcaacga gtggatcgag atcgcccatc tgatccaccg caaggccgtc tatgagctgc 661 atggcgaaaa cgatccggtg ccggcattgt cgccgcgcga gatcgagtgt ctgcactgga 721 ccgccctcgg caaggattac aaggatattt cggtcatcct gggcatatca gagcatacca 781 cacgcgatta cctgaagacc gcccgcttca agctcggctg cgccacgatc tcggccgccg 841 cgtcgcgggc tgttcaattg cgcatcatca atcccgctgc aaacgacgaa aactacgctt 901 tagtagctta ataacgctga tagtgctagt gtagatcgct actagagaaa gaggagaaat 961 actagatgtt cgttatcatc caggctcacg aataccagaa atacgctgct gttctggacc 1021 agatgttccg tctgcgtaaa aaagttttcg ctgacaccct gtgctgggac gttccggtta 1081 tcggtccgta cgaacgtgac tcctacgact ccctggctcc ggcttacctg gtttggtgca 1141 acgactcccg tacccgtctg tacggtggta tgcgtctgat gccgaccacc ggtccgaccc 1201 tgctgtacga cgttttccgt gaaaccttcc cggacgctgc tgacctgatc gctccgggta 1261 tctgggaagg tacccgtatg tgcatcgacg aagaagctat cgctaaagac ttcccggaaa 1321 tcgacgctgg tcgtgctttc tccatgatgc tgctggctct gtgcgaatgc gctctggacc 1381 acggtatcca caccatgatc tccaactacg aaccgtacct gaaacgtgtt tacaaacgtg 1441 ctggtgctga agttgaagaa ctgggtcgtg ctgacggtta cggtaaatac ccggtttgct 1501 gcggtgcttt cgaagtttcc gaccgtgttc tgcgtaaaat gcgtgctgct ctgggtctga 1561 ccctgccgct gtacgttcgt cacgttccgg ctcgttccgt tgttacccag ttcctggaaa 1621 tggctgctgc tgctaacgac gaaaactacg ctctggttgc ttaataaccc tgatagtgct 1681 agtgtagatc cctactagag aaagaggaga aatactagag ttaggtcgag gtggcccggc 1741 tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc 1801 atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc 1861 caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt 1921 cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag 1981 cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt 2041 agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg 2101 tggcggggcc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg 2161 acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg 2221 ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga 2281 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg 2341 gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc 2401 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc 2461 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc 2521 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg 2581 cgccggtgat gccggccacg atgcgtccgg cgtagaggat ccacaggacg ggtgtggtcg 2641 ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc 2701 caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata 2761 gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga 2821 ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga 2881 ggatgacgat gagcgcattg ttagatttca ttactagaga aagaggagaa atactagatg 2941 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 3001 gacgtgaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 3061 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 3121 gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag 3181 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 3241 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 3301 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 3361 ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc 3421 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 3481 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 3541 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 3601 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaataa //