BBa_S04391
type: ComponentDefinition
persistent identity: BBa S04391
display id: BBa_S04391
version: 1
was generated by: iGEM to SBOL conversion
description: S04390:E0020
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_E0020
component: BBa_S04390
sequence annotation: BBa_S04390
sequence annotation: BBa_E0020
sequence: https://synbiohub.org/public/igem/BBa_S04391_sequence/1

BBa_E0020
type: Component
persistent identity: component2277159
display id: component2277159
version: 1
top level: BBa_S04391
definition: ecfp
access: public

BBa_S04390
type: Component
persistent identity: component2277158
display id: component2277158
version: 1
top level: BBa_S04391
definition: BBa_S04390
access: public

BBa_S04390
type: SequenceAnnotation
persistent identity: annotation2277158
display id: annotation2277158
version: 1
top level: BBa_S04391
location: https://synbiohub.org/public/igem/BBa_S04391/annotation2277158/range2277158/1
component: BBa_S04390

https://synbiohub.org/public/igem/BBa_S04391/annotation2277158/range2277158/1
type: Range
persistent identity: range2277158
display id: range2277158
version: 1
top level: BBa_S04391
direction: direction/reverse
start: 1
end: 2931
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation2277159
display id: annotation2277159
version: 1
top level: BBa_S04391
location: https://synbiohub.org/public/igem/BBa_S04391/annotation2277159/range2277159/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_S04391/annotation2277159/range2277159/1
type: Range
persistent identity: range2277159
display id: range2277159
version: 1
top level: BBa_S04391
direction: direction/reverse
start: 2938
end: 3660
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: iGEM to SBOL conversion
description: engineered cyan fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

BBa_S04390
type: ComponentDefinition
persistent identity: BBa S04390
display id: BBa_S04390
version: 1
was generated by: iGEM to SBOL conversion
description: S04389:B0034
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_S04389
sequence annotation: BBa_S04389
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04390_sequence/1

BBa_B0034
type: Component
persistent identity: component2272381
display id: component2272381
version: 1
top level: BBa_S04390
definition: BBa_B0034
access: public

BBa_S04389
type: Component
persistent identity: component2272379
display id: component2272379
version: 1
top level: BBa_S04390
definition: BBa_S04389
access: public

BBa_S04389
type: SequenceAnnotation
persistent identity: annotation2272379
display id: annotation2272379
version: 1
top level: BBa_S04390
location: https://synbiohub.org/public/igem/BBa_S04390/annotation2272379/range2272379/1
component: BBa_S04389

https://synbiohub.org/public/igem/BBa_S04390/annotation2272379/range2272379/1
type: Range
persistent identity: range2272379
display id: range2272379
version: 1
top level: BBa_S04390
direction: direction/reverse
start: 1
end: 2911
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2272381
display id: annotation2272381
version: 1
top level: BBa_S04390
location: https://synbiohub.org/public/igem/BBa_S04390/annotation2272381/range2272381/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04390/annotation2272381/range2272381/1
type: Range
persistent identity: range2272381
display id: range2272381
version: 1
top level: BBa_S04390
direction: direction/reverse
start: 2920
end: 2931
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_S04389
type: ComponentDefinition
persistent identity: BBa S04389
display id: BBa_S04389
version: 1
was generated by: iGEM to SBOL conversion
description: S04388:J31006
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J31006
component: BBa_S04388
sequence annotation: BBa_S04388
sequence annotation: BBa_J31006
sequence: https://synbiohub.org/public/igem/BBa_S04389_sequence/1

BBa_J31006
type: Component
persistent identity: component2268081
display id: component2268081
version: 1
top level: BBa_S04389
definition: tetA(C)b
access: public

BBa_S04388
type: Component
persistent identity: component2268076
display id: component2268076
version: 1
top level: BBa_S04389
definition: BBa_S04388
access: public

BBa_S04388
type: SequenceAnnotation
persistent identity: annotation2268076
display id: annotation2268076
version: 1
top level: BBa_S04389
location: https://synbiohub.org/public/igem/BBa_S04389/annotation2268076/range2268076/1
component: BBa_S04388

https://synbiohub.org/public/igem/BBa_S04389/annotation2268076/range2268076/1
type: Range
persistent identity: range2268076
display id: range2268076
version: 1
top level: BBa_S04389
direction: direction/reverse
start: 1
end: 1712
orientation: inline

BBa_J31006
type: SequenceAnnotation
persistent identity: annotation2268081
display id: annotation2268081
version: 1
top level: BBa_S04389
location: https://synbiohub.org/public/igem/BBa_S04389/annotation2268081/range2268081/1
component: BBa_J31006

https://synbiohub.org/public/igem/BBa_S04389/annotation2268081/range2268081/1
type: Range
persistent identity: range2268081
display id: range2268081
version: 1
top level: BBa_S04389
direction: direction/reverse
start: 1721
end: 2911
orientation: inline

tetA(C)b
type: ComponentDefinition
persistent identity: BBa J31006
display id: BBa_J31006
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This is the coding region for Tetracyline resistance in the reverse orientation. If a promoter adn RBS are placed downstream and the entire region, from promoter to stop codon is flipped the cell will express tetracycline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plasmids.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: stop
sequence annotation: tetA(C) backwards
sequence annotation: Rev primer J31006
sequence annotation: Fwd primer J31006
sequence: https://synbiohub.org/public/igem/BBa_J31006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1910574
display id: annotation1910574
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
type: Range
persistent identity: range1910574
display id: range1910574
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1
end: 3
orientation: inline

tetA(C) backwards
type: SequenceAnnotation
persistent identity: annotation1884998
display id: annotation1884998
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
type: Range
persistent identity: range1884998
display id: range1884998
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1
end: 1191
orientation: inline

Rev primer J31006
type: SequenceAnnotation
persistent identity: annotation1910575
display id: annotation1910575
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
type: Range
persistent identity: range1910575
display id: range1910575
version: 1
top level: tetA(C)b
direction: direction/forward
start: 1
end: 20
orientation: inline

Fwd primer J31006
type: SequenceAnnotation
persistent identity: annotation1910576
display id: annotation1910576
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
type: Range
persistent identity: range1910576
display id: range1910576
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1168
end: 1191
orientation: inline

BBa_S04388
type: ComponentDefinition
persistent identity: BBa S04388
display id: BBa_S04388
version: 1
was generated by: iGEM to SBOL conversion
description: S04387:B0034
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_S04387
sequence annotation: BBa_S04387
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04388_sequence/1

BBa_B0034
type: Component
persistent identity: component2263334
display id: component2263334
version: 1
top level: BBa_S04388
definition: BBa_B0034
access: public

BBa_S04387
type: Component
persistent identity: component2263332
display id: component2263332
version: 1
top level: BBa_S04388
definition: BBa_S04387
access: public

BBa_S04387
type: SequenceAnnotation
persistent identity: annotation2263332
display id: annotation2263332
version: 1
top level: BBa_S04388
location: https://synbiohub.org/public/igem/BBa_S04388/annotation2263332/range2263332/1
component: BBa_S04387

https://synbiohub.org/public/igem/BBa_S04388/annotation2263332/range2263332/1
type: Range
persistent identity: range2263332
display id: range2263332
version: 1
top level: BBa_S04388
direction: direction/reverse
start: 1
end: 1692
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2263334
display id: annotation2263334
version: 1
top level: BBa_S04388
location: https://synbiohub.org/public/igem/BBa_S04388/annotation2263334/range2263334/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04388/annotation2263334/range2263334/1
type: Range
persistent identity: range2263334
display id: range2263334
version: 1
top level: BBa_S04388
direction: direction/reverse
start: 1701
end: 1712
orientation: inline

BBa_S04387
type: ComponentDefinition
persistent identity: BBa S04387
display id: BBa_S04387
version: 1
was generated by: iGEM to SBOL conversion
description: S04386:C0076
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0076
component: BBa_S04386
sequence annotation: BBa_S04386
sequence annotation: BBa_C0076
sequence: https://synbiohub.org/public/igem/BBa_S04387_sequence/1

BBa_C0076
type: Component
persistent identity: component2256783
display id: component2256783
version: 1
top level: BBa_S04387
definition: cinI
access: public

BBa_S04386
type: Component
persistent identity: component2256779
display id: component2256779
version: 1
top level: BBa_S04387
definition: BBa_S04386
access: public

BBa_S04386
type: SequenceAnnotation
persistent identity: annotation2256779
display id: annotation2256779
version: 1
top level: BBa_S04387
location: https://synbiohub.org/public/igem/BBa_S04387/annotation2256779/range2256779/1
component: BBa_S04386

https://synbiohub.org/public/igem/BBa_S04387/annotation2256779/range2256779/1
type: Range
persistent identity: range2256779
display id: range2256779
version: 1
top level: BBa_S04387
direction: direction/reverse
start: 1
end: 959
orientation: inline

BBa_C0076
type: SequenceAnnotation
persistent identity: annotation2256783
display id: annotation2256783
version: 1
top level: BBa_S04387
location: https://synbiohub.org/public/igem/BBa_S04387/annotation2256783/range2256783/1
component: BBa_C0076

https://synbiohub.org/public/igem/BBa_S04387/annotation2256783/range2256783/1
type: Range
persistent identity: range2256783
display id: range2256783
version: 1
top level: BBa_S04387
direction: direction/reverse
start: 966
end: 1692
orientation: inline

cinI
type: ComponentDefinition
persistent identity: BBa C0076
display id: BBa_C0076
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: cinI codes for an autoinducer synthetase which utilizes SAM to make O3-C14:1-HSL. In complex with O3-C14:1-HSL, CinR (BBa_C0077) binds to the Cin promoter (BBa_R0077) and activates transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tga -> tAaTAA
star: true
creator: crackdots
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: CinI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0076_sequence/1

CinI
type: SequenceAnnotation
persistent identity: annotation301035
display id: annotation301035
version: 1
top level: cinI
location: https://synbiohub.org/public/igem/BBa_C0076/annotation301035/range301035/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0076/annotation301035/range301035/1
type: Range
persistent identity: range301035
display id: range301035
version: 1
top level: cinI
direction: direction/forward
start: 1
end: 663
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306586
display id: annotation306586
version: 1
top level: cinI
location: https://synbiohub.org/public/igem/BBa_C0076/annotation306586/range306586/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0076/annotation306586/range306586/1
type: Range
persistent identity: range306586
display id: range306586
version: 1
top level: cinI
direction: direction/forward
start: 664
end: 696
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214001
display id: annotation2214001
version: 1
top level: cinI
location: https://synbiohub.org/public/igem/BBa_C0076/annotation2214001/range2214001/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0076/annotation2214001/range2214001/1
type: Range
persistent identity: range2214001
display id: range2214001
version: 1
top level: cinI
direction: direction/forward
start: 703
end: 727
orientation: inline

BBa_S04386
type: ComponentDefinition
persistent identity: BBa S04386
display id: BBa_S04386
version: 1
was generated by: iGEM to SBOL conversion
description: S04385:B0034
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_S04385
sequence annotation: BBa_S04385
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04386_sequence/1

BBa_B0034
type: Component
persistent identity: component2250021
display id: component2250021
version: 1
top level: BBa_S04386
definition: BBa_B0034
access: public

BBa_S04385
type: Component
persistent identity: component2250019
display id: component2250019
version: 1
top level: BBa_S04386
definition: BBa_S04385
access: public

BBa_S04385
type: SequenceAnnotation
persistent identity: annotation2250019
display id: annotation2250019
version: 1
top level: BBa_S04386
location: https://synbiohub.org/public/igem/BBa_S04386/annotation2250019/range2250019/1
component: BBa_S04385

https://synbiohub.org/public/igem/BBa_S04386/annotation2250019/range2250019/1
type: Range
persistent identity: range2250019
display id: range2250019
version: 1
top level: BBa_S04386
direction: direction/reverse
start: 1
end: 939
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2250021
display id: annotation2250021
version: 1
top level: BBa_S04386
location: https://synbiohub.org/public/igem/BBa_S04386/annotation2250021/range2250021/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04386/annotation2250021/range2250021/1
type: Range
persistent identity: range2250021
display id: range2250021
version: 1
top level: BBa_S04386
direction: direction/reverse
start: 948
end: 959
orientation: inline

BBa_S04385
type: ComponentDefinition
persistent identity: BBa S04385
display id: BBa_S04385
version: 1
was generated by: iGEM to SBOL conversion
description: S04384:C0077
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0077
component: BBa_S04384
sequence annotation: BBa_S04384
sequence annotation: BBa_C0077
sequence: https://synbiohub.org/public/igem/BBa_S04385_sequence/1

BBa_C0077
type: Component
persistent identity: component2247002
display id: component2247002
version: 1
top level: BBa_S04385
definition: cinr
access: public

BBa_S04384
type: Component
persistent identity: component2246998
display id: component2246998
version: 1
top level: BBa_S04385
definition: BBa_S04384
access: public

BBa_S04384
type: SequenceAnnotation
persistent identity: annotation2246998
display id: annotation2246998
version: 1
top level: BBa_S04385
location: https://synbiohub.org/public/igem/BBa_S04385/annotation2246998/range2246998/1
component: BBa_S04384

https://synbiohub.org/public/igem/BBa_S04385/annotation2246998/range2246998/1
type: Range
persistent identity: range2246998
display id: range2246998
version: 1
top level: BBa_S04385
direction: direction/reverse
start: 1
end: 146
orientation: inline

BBa_C0077
type: SequenceAnnotation
persistent identity: annotation2247002
display id: annotation2247002
version: 1
top level: BBa_S04385
location: https://synbiohub.org/public/igem/BBa_S04385/annotation2247002/range2247002/1
component: BBa_C0077

https://synbiohub.org/public/igem/BBa_S04385/annotation2247002/range2247002/1
type: Range
persistent identity: range2247002
display id: range2247002
version: 1
top level: BBa_S04385
direction: direction/reverse
start: 153
end: 939
orientation: inline

cinr
type: ComponentDefinition
persistent identity: BBa C0077
display id: BBa_C0077
version: 1
was generated by: iGEM to SBOL conversion
description: cinR activator from Rhizobium leguminosarum (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with O3-C14:1-HSL (made by BBa_C0076), CinR binds to the Cin promoter (BBa_R0077) and activates transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tag -> taATAA
star: true
creator: crackdots
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cinR
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0077_sequence/1

cinR
type: SequenceAnnotation
persistent identity: annotation301112
display id: annotation301112
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1
type: Range
persistent identity: range301112
display id: range301112
version: 1
top level: cinr
direction: direction/forward
start: 1
end: 723
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306600
display id: annotation306600
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1
type: Range
persistent identity: range306600
display id: range306600
version: 1
top level: cinr
direction: direction/forward
start: 724
end: 756
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214000
display id: annotation2214000
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1
type: Range
persistent identity: range2214000
display id: range2214000
version: 1
top level: cinr
direction: direction/forward
start: 763
end: 787
orientation: inline

BBa_S04384
type: ComponentDefinition
persistent identity: BBa S04384
display id: BBa_S04384
version: 1
was generated by: iGEM to SBOL conversion
description: K091146:B0034
created: 2009-12-07T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 3663
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Eniak Hernandez
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_K091146
sequence annotation: BBa_K091146
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04384_sequence/1

BBa_B0034
type: Component
persistent identity: component2063899
display id: component2063899
version: 1
top level: BBa_S04384
definition: BBa_B0034
access: public

BBa_K091146
type: Component
persistent identity: component2063897
display id: component2063897
version: 1
top level: BBa_S04384
definition: BBa_K091146
access: public

BBa_K091146
type: SequenceAnnotation
persistent identity: annotation2063897
display id: annotation2063897
version: 1
top level: BBa_S04384
location: https://synbiohub.org/public/igem/BBa_S04384/annotation2063897/range2063897/1
component: BBa_K091146

https://synbiohub.org/public/igem/BBa_S04384/annotation2063897/range2063897/1
type: Range
persistent identity: range2063897
display id: range2063897
version: 1
top level: BBa_S04384
direction: direction/reverse
start: 1
end: 126
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2063899
display id: annotation2063899
version: 1
top level: BBa_S04384
location: https://synbiohub.org/public/igem/BBa_S04384/annotation2063899/range2063899/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04384/annotation2063899/range2063899/1
type: Range
persistent identity: range2063899
display id: range2063899
version: 1
top level: BBa_S04384
direction: direction/reverse
start: 135
end: 146
orientation: inline

BBa_K091146
type: ComponentDefinition
persistent identity: BBa K091146
display id: BBa_K091146
version: 1
was generated by: iGEM to SBOL conversion
description: pLas/Lux Hybrid Promoter
created: 2008-07-16T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: The LuxR binding site is from BBa_R0062 and the pLas' promoter is from the region upstream of the LasI gene in Pseudomonas aeruginosa.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: K091146 is a modified version of the pLas' promoter (BBa_K091117) containing the addition of a luxR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of PAI-1 and LasR and repressed in the presence of AI-1 and LuxR.
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The Lux box was placed adjacent to the LasR binding site with the aim of minimizing cross-talk in the presence of both PAI-1 and AI-1; since the LasR site should have a higher affinity, we hope that the binding of PAI-1 will competitively exclude the binding of AI-1.
star: true
creator: Erin Feeney
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: LasR
sequence annotation: -35
sequence annotation: LuxR
sequence annotation: -10
sequence annotation: Transcriptional Start
sequence: https://synbiohub.org/public/igem/BBa_K091146_sequence/1

LasR
type: SequenceAnnotation
persistent identity: annotation1967354
display id: annotation1967354
version: 1
top level: BBa_K091146
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1
type: Range
persistent identity: range1967354
display id: range1967354
version: 1
top level: BBa_K091146
direction: direction/forward
start: 75
end: 93
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1967357
display id: annotation1967357
version: 1
top level: BBa_K091146
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1
type: Range
persistent identity: range1967357
display id: range1967357
version: 1
top level: BBa_K091146
direction: direction/forward
start: 86
end: 91
orientation: inline

LuxR
type: SequenceAnnotation
persistent identity: annotation1967355
display id: annotation1967355
version: 1
top level: BBa_K091146
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1
type: Range
persistent identity: range1967355
display id: range1967355
version: 1
top level: BBa_K091146
direction: direction/forward
start: 94
end: 113
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1967356
display id: annotation1967356
version: 1
top level: BBa_K091146
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1
type: Range
persistent identity: range1967356
display id: range1967356
version: 1
top level: BBa_K091146
direction: direction/forward
start: 113
end: 118
orientation: inline

Transcriptional Start
type: SequenceAnnotation
persistent identity: annotation1967358
display id: annotation1967358
version: 1
top level: BBa_K091146
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1
type: Range
persistent identity: range1967358
display id: range1967358
version: 1
top level: BBa_K091146
direction: direction/forward
start: 125
end: 125
orientation: inline

https://synbiohub.org/public/igem/BBa_S04391_sequence/1
type: Sequence
persistent identity: BBa S04391 sequence
display id: BBa_S04391_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgatagtgctagtgtagatccctactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatgggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0020_sequence/1
type: Sequence
persistent identity: BBa E0020 sequence
display id: BBa_E0020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04390_sequence/1
type: Sequence
persistent identity: BBa S04390 sequence
display id: BBa_S04390_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgatagtgctagtgtagatccctactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatgggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04389_sequence/1
type: Sequence
persistent identity: BBa S04389 sequence
display id: BBa_S04389_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgatagtgctagtgtagatccctactagagaaagaggagaaatactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatgggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31006_sequence/1
type: Sequence
persistent identity: BBa J31006 sequence
display id: BBa_J31006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04388_sequence/1
type: Sequence
persistent identity: BBa S04388 sequence
display id: BBa_S04388_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgatagtgctagtgtagatccctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04387_sequence/1
type: Sequence
persistent identity: BBa S04387 sequence
display id: BBa_S04387_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0076_sequence/1
type: Sequence
persistent identity: BBa C0076 sequence
display id: BBa_C0076_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04386_sequence/1
type: Sequence
persistent identity: BBa S04386 sequence
display id: BBa_S04386_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04385_sequence/1
type: Sequence
persistent identity: BBa S04385 sequence
display id: BBa_S04385_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0077_sequence/1
type: Sequence
persistent identity: BBa C0077 sequence
display id: BBa_C0077_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04384_sequence/1
type: Sequence
persistent identity: BBa S04384 sequence
display id: BBa_S04384_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091146_sequence/1
type: Sequence
persistent identity: BBa K091146 sequence
display id: BBa_K091146_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z