LOCUS       BBa_S04400              2970 bp    DNA     linear       15-Nov-2024
DEFINITION  S04399:B0034
ACCESSION   BBa_S04400
VERSION     BBa_S04400.1  
FEATURES             Location/Qualifiers
     RBS             226..231
                     /label=embedded RBS
     stem_loop       35..74
                     /label=stem_loop
     stem_loop       91..136
                     /label=stem_loop
     promoter        1..220
                     /label=unidentified/uncharacterized CinR dependent
     promoter        1..231
                     /label=BBa_R0077
     misc_feature    244..247
                     /label=conserved
     RBS             240..251
                     /label=BBa_B0034
     misc_feature    1..251
                     /label=BBa_S04394
     CDS             258..974
                     /label=lasR
     misc_feature    975..1007
                     /label=LVA
     misc_feature    737..737
                     /label=G
     misc_feature    1014..1038
                     /label=Help:Barcodes
     CDS             258..1038
                     /label=BBa_C0079
     misc_feature    1..1038
                     /label=BBa_S04395
     misc_feature    1051..1054
                     /label=conserved
     RBS             1047..1058
                     /label=BBa_B0034
     misc_feature    1..1058
                     /label=BBa_S04396
     CDS             1065..1667
                     /label=LasI
     misc_feature    1668..1700
                     /label=LVA
     misc_feature    1707..1731
                     /label=Help:Barcodes
     CDS             1065..1731
                     /label=BBa_C0078
     misc_feature    1..1731
                     /label=BBa_S04397
     misc_feature    1744..1747
                     /label=conserved
     RBS             1740..1751
                     /label=BBa_B0034
     misc_feature    1..1751
                     /label=BBa_S04398
     CDS             1760..2950
                     /label=tetA(C) backwards
     misc_feature    1760..1762
                     /label=stop
     primer_bind     1760..1779
                     /label=Rev primer J31006
     primer_bind     2927..2950
                     /label=Fwd primer J31006
     CDS             1760..2950
                     /label=BBa_J31006
     misc_feature    1..2950
                     /label=BBa_S04399
     misc_feature    2963..2966
                     /label=conserved
     RBS             2959..2970
                     /label=BBa_B0034
ORIGIN
        1 ccctttgtgc gtccaaacgg acgcacggcg ctctaaagcg ggtcgcgatc tttcagattc
       61 gctcctcgcg ctttcagtct ttgttttggc gcatgtcgtt atcgcaaaac cgctgcacac
      121 ttttgcgcga catgctctga tccccctcat ctgggggggc ctatctgagg gaatttccga
      181 tccggctcgc ctgaaccatt ctgctttcca cgaacttgaa aacgctggag gtactagaga
      241 aagaggagaa atactagatg gccttggttg acggttttct tgagctggaa cgctcaagtg
      301 gaaaattgga gtggagcgcc atcctccaga agatggcgag cgaccttgga ttctcgaaga
      361 tcctgttcgg cctgttgcct aaggacagcc aggactacga gaacgccttc atcgtcggca
      421 actacccggc cgcctggcgc gagcattacg accgggctgg ctacgcgcgg gtcgacccga
      481 cggtcagtca ctgtacccag agcgtactgc cgattttctg ggaaccgtcc atctaccaga
      541 cgcgaaagca gcacgagttc ttcgaggaag cctcggccgc cggcctggtg tatgggctga
      601 ccatgccgct gcatggtgct cgcggcgaac tcggcgcgct gagcctcagc gtggaagcgg
      661 aaaaccgggc cgaggccaac cgtttcatag agtcggtcct gccgaccctg tggatgctca
      721 aggactacgc actgcaaagc ggtgccggac tggccttcga acatccggtc agcaaaccgg
      781 tggttctgac cagccgggag aaggaagtgt tgcagtggtg cgccatcggc aagaccagtt
      841 gggagatatc ggttatctgc aactgctcgg aagccaatgt gaacttccat atgggaaata
      901 ttcggcggaa gttcggtgtg acctcccgcc gcgtagcggc cattatggcc gttaatttgg
      961 gtcttattac tctcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat
     1021 agtgctagtg tagatcacta ctagagaaag aggagaaata ctagatgatc gttcagatcg
     1081 gtcgtcgtga agagttcgac aaaaaactgc tgggtgaaat gcacaaactg cgtgctcagg
     1141 ttttcaaaga acgtaaaggt tgggacgttt ccgttatcga cgaaatggaa atcgacggtt
     1201 acgacgctct gtccccgtac tacatgctga tccaggaaga caccccggaa gctcaggttt
     1261 tcggttgctg gcgtatcttc gacaccaccg gtccgtacat gctgaaaaac accttcccgg
     1321 aactgctgca cggtaaagaa gctccgtgct ccccgcacat ctgggaactg tcccgtttcg
     1381 ctatcaactc cggtcagaaa ggttccctgg gtttctccga ctgcaccctg gaagctatgc
     1441 gtgctctggc tcgttactcc ttgcagaacg acatccagac cctggttacc gttaccaccg
     1501 ttggtgttga aaaaatgatg atccgtgctg gtctggacgt ttcccgtttc ggtccgcacc
     1561 tgaaaatcgg tatcgaacgt gctgttgctc tgcgtatcga actgaacgct aaaacccaga
     1621 tcgctctgta cggtggtgtt ctggttgaac agcgtctggc tgtttccgct gctaacgacg
     1681 aaaactacgc tctggttgct taataactct gatagtgcta gtgtagatct ctactagaga
     1741 aagaggagaa atactagagt taggtcgagg tggcccggct ccatgcaccg cgacgcaacg
     1801 cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc
     1861 tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa gttaggctgg
     1921 taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca
     1981 gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga
     2041 aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca
     2101 tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcggggcca gtgacgaagg
     2161 cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc
     2221 tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga
     2281 gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc
     2341 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg
     2401 actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa
     2461 ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     2521 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     2581 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     2641 tgcgtccggc gtagaggatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
     2701 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc
     2761 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt
     2821 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa
     2881 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
     2941 tagatttcat tactagagaa agaggagaaa
//