LOCUS       BBa_S04408              2209 bp    DNA     linear       16-Dec-2024
DEFINITION  S04407:R0040
ACCESSION   BBa_S04408
VERSION     BBa_S04408.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_S04403
     CDS             230..846
                     /label=tetR
     misc_feature    847..880
                     /label=SsrA
     misc_feature    887..911
                     /label=Help:Barcodes
     CDS             227..911
                     /label=BBa_C0040
     misc_feature    1..911
                     /label=BBa_S04404
     promoter        957..962
                     /label=-10
     promoter        934..939
                     /label=-35
     misc_feature    944..960
                     /label=OR1
     misc_feature    920..936
                     /label=OR2
     misc_feature    920..968
                     /label=BBa_R0051
     promoter        920..968
                     /label=BBa_R0051
     misc_feature    1..968
                     /label=BBa_S04405
     misc_feature    981..984
                     /label=conserved
     RBS             977..988
                     /label=BBa_B0034
     misc_feature    1..988
                     /label=BBa_S04406
     misc_feature    2084..2122
                     /label=LVA
     CDS             995..2122
                     /label=lacI-LVA
     misc_feature    995..2122
                     /label=BBa_C0012
     misc_feature    2123..2147
                     /label=Help:Barcodes
     CDS             995..2122
                     /label=BBa_C0012
     misc_feature    1..2147
                     /label=BBa_S04407
     misc_feature    2156..2174
                     /label=TetR 1
     misc_feature    2156..2209
                     /label=BBa_R0040
     promoter        2175..2180
                     /label=-35
     misc_feature    2181..2199
                     /label=TetR 2
     promoter        2198..2203
                     /label=-10
     promoter        2156..2209
                     /label=BBa_R0040
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ccagattaga
      241 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt
      301 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt
      361 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac
      421 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag
      481 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc
      541 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt
      601 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt
      661 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag
      721 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc
      781 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag
      841 tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta
      901 gtgtagatca ctactagagt aacaccgtgc gtgttgacta ttttacctct ggcggtgata
      961 atggttgcta ctagagaaag aggagaaata ctagatggtg aatgtgaaac cagtaacgtt
     1021 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
     1081 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
     1141 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
     1201 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
     1261 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
     1321 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
     1381 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
     1441 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
     1501 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
     1561 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
     1621 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
     1681 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
     1741 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
     1801 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
     1861 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
     1921 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
     1981 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2041 aatgcagctg gcacgacagg tttcccgact ggaaagcggg caggctgcaa acgacgaaaa
     2101 ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac tagagtccct
     2161 atcagtgata gagattgaca tccctatcag tgatagagat actgagcac
//