LOCUS       BBa_S04409              1048 bp    DNA     linear       15-Nov-2024
DEFINITION  S04404:B0015
ACCESSION   BBa_S04409
VERSION     BBa_S04409.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_S04403
     CDS             230..846
                     /label=tetR
     misc_feature    847..880
                     /label=SsrA
     misc_feature    887..911
                     /label=Help:Barcodes
     CDS             227..911
                     /label=BBa_C0040
     misc_feature    1..911
                     /label=BBa_S04404
     stem_loop       931..974
                     /label=stem_loop
     misc_feature    920..999
                     /label=BBa_B0010
     terminator      920..999
                     /label=BBa_B0010
     stem_loop       1015..1034
                     /label=T7 TE
     misc_feature    1041..1041
                     /label=stop
     polyA_site      1035..1048
                     /label=polya
     misc_feature    1008..1048
                     /label=BBa_B0012
     terminator      1008..1048
                     /label=BBa_B0012
     terminator      920..1048
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ccagattaga
      241 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt
      301 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt
      361 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac
      421 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag
      481 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc
      541 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt
      601 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt
      661 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag
      721 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc
      781 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag
      841 tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta
      901 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      961 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1021 ccttcgggtg ggcctttctg cgtttata
//