LOCUS       BBa_S04412              2284 bp    DNA     linear       15-Nov-2024
DEFINITION  S04411:C0012
ACCESSION   BBa_S04412
VERSION     BBa_S04412.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_S04403
     CDS             230..846
                     /label=tetR
     misc_feature    847..880
                     /label=SsrA
     misc_feature    887..911
                     /label=Help:Barcodes
     CDS             227..911
                     /label=BBa_C0040
     misc_feature    1..911
                     /label=BBa_S04404
     stem_loop       931..974
                     /label=stem_loop
     misc_feature    920..999
                     /label=BBa_B0010
     terminator      920..999
                     /label=BBa_B0010
     stem_loop       1015..1034
                     /label=T7 TE
     misc_feature    1041..1041
                     /label=stop
     polyA_site      1035..1048
                     /label=polya
     misc_feature    1008..1048
                     /label=BBa_B0012
     terminator      1008..1048
                     /label=BBa_B0012
     terminator      920..1048
                     /label=BBa_B0015
     misc_feature    1..1048
                     /label=BBa_S04409
     promoter        1094..1099
                     /label=-10
     promoter        1071..1076
                     /label=-35
     misc_feature    1081..1097
                     /label=OR1
     misc_feature    1057..1073
                     /label=OR2
     misc_feature    1057..1105
                     /label=BBa_R0051
     promoter        1057..1105
                     /label=BBa_R0051
     misc_feature    1..1105
                     /label=BBa_S04410
     misc_feature    1118..1121
                     /label=conserved
     RBS             1114..1125
                     /label=BBa_B0034
     misc_feature    1..1125
                     /label=BBa_S04411
     misc_feature    2221..2259
                     /label=LVA
     CDS             1132..2259
                     /label=lacI-LVA
     misc_feature    1132..2259
                     /label=BBa_C0012
     misc_feature    2260..2284
                     /label=Help:Barcodes
     CDS             1132..2259
                     /label=BBa_C0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ccagattaga
      241 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt
      301 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt
      361 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac
      421 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag
      481 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc
      541 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt
      601 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt
      661 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag
      721 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc
      781 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag
      841 tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta
      901 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      961 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1021 ccttcgggtg ggcctttctg cgtttatata ctagagtaac accgtgcgtg ttgactattt
     1081 tacctctggc ggtgataatg gttgctacta gagaaagagg agaaatacta gatggtgaat
     1141 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
     1201 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
     1261 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
     1321 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
     1381 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
     1441 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
     1501 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
     1561 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
     1621 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
     1681 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
     1741 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
     1801 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
     1861 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
     1921 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
     1981 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
     2041 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
     2101 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     2161 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     2221 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
     2281 tctc
//