LOCUS BBa_S04413 2421 bp DNA linear 05-Oct-2024 DEFINITION S04412:B0015 ACCESSION BBa_S04413 VERSION BBa_S04413.1 FEATURES Location/Qualifiers stem_loop 931..974 /label=stem_loop misc_feature 920..999 /label=BBa_B0010 terminator 920..999 /label=BBa_B0010 stem_loop 1015..1034 /label=T7 TE misc_feature 1041..1041 /label=stop polyA_site 1035..1048 /label=polya misc_feature 1008..1048 /label=BBa_B0012 terminator 1008..1048 /label=BBa_B0012 terminator 920..1048 /label=BBa_B0015 CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_S04403 CDS 230..846 /label=tetR misc_feature 847..880 /label=SsrA misc_feature 887..911 /label=Help:Barcodes CDS 227..911 /label=BBa_C0040 misc_feature 1..911 /label=BBa_S04404 stem_loop 931..974 /label=stem_loop misc_feature 920..999 /label=BBa_B0010 terminator 920..999 /label=BBa_B0010 stem_loop 1015..1034 /label=T7 TE misc_feature 1041..1041 /label=stop polyA_site 1035..1048 /label=polya misc_feature 1008..1048 /label=BBa_B0012 terminator 1008..1048 /label=BBa_B0012 terminator 920..1048 /label=BBa_B0015 misc_feature 1..1048 /label=BBa_S04409 promoter 1094..1099 /label=-10 promoter 1071..1076 /label=-35 misc_feature 1081..1097 /label=OR1 misc_feature 1057..1073 /label=OR2 misc_feature 1057..1105 /label=BBa_R0051 promoter 1057..1105 /label=BBa_R0051 misc_feature 1..1105 /label=BBa_S04410 misc_feature 1118..1121 /label=conserved RBS 1114..1125 /label=BBa_B0034 misc_feature 1..1125 /label=BBa_S04411 misc_feature 2221..2259 /label=LVA CDS 1132..2259 /label=lacI-LVA misc_feature 1132..2259 /label=BBa_C0012 misc_feature 2260..2284 /label=Help:Barcodes CDS 1132..2259 /label=BBa_C0012 misc_feature 1..2284 /label=BBa_S04412 stem_loop 2304..2347 /label=stem_loop misc_feature 2293..2372 /label=BBa_B0010 terminator 2293..2372 /label=BBa_B0010 stem_loop 2388..2407 /label=T7 TE misc_feature 2414..2414 /label=stop polyA_site 2408..2421 /label=polya misc_feature 2381..2421 /label=BBa_B0012 terminator 2381..2421 /label=BBa_B0012 terminator 2293..2421 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ccagattaga 241 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt 301 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt 361 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac 421 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag 481 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc 541 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt 601 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt 661 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag 721 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc 781 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag 841 tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta 901 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 961 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 1021 ccttcgggtg ggcctttctg cgtttatata ctagagtaac accgtgcgtg ttgactattt 1081 tacctctggc ggtgataatg gttgctacta gagaaagagg agaaatacta gatggtgaat 1141 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt 1201 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg 1261 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag 1321 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc 1381 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa 1441 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt 1501 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc 1561 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt 1621 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag 1681 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc 1741 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg 1801 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact 1861 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc 1921 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca 1981 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc 2041 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc 2101 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc 2161 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 2221 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga 2281 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 2341 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 2401 gtgggccttt ctgcgtttat a //