##gff-version 3
##sequence-region BBa_S04414 1 2483
BBa_S04414	.	engineered_region	1	2421	.	+	0	ID=BBa_S04413;Name=BBa_S04413
BBa_S04414	.	engineered_region	1	2284	.	+	0	ID=BBa_S04412;Name=BBa_S04412;Parent=BBa_S04413
BBa_S04414	.	CDS	1132	2259	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S04412
BBa_S04414	.	engineered_tag	2260	2284	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S04414	.	CDS	1132	2259	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S04414	.	engineered_region	1132	2259	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S04414	.	tag	2221	2259	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S04414	.	engineered_region	1	1125	.	+	0	ID=BBa_S04411;Name=BBa_S04411;Parent=BBa_S04412
BBa_S04414	.	ribosome_entry_site	1114	1125	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04411
BBa_S04414	.	conserved_region	1118	1121	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04414	.	engineered_region	1	1105	.	+	0	ID=BBa_S04410;Name=BBa_S04410;Parent=BBa_S04411
BBa_S04414	.	engineered_region	1	1048	.	+	0	ID=BBa_S04409;Name=BBa_S04409;Parent=BBa_S04410
BBa_S04414	.	terminator	920	1048	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S04409
BBa_S04414	.	terminator	920	999	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04414	.	stem_loop	931	974	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04414	.	engineered_region	920	999	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04414	.	terminator	1008	1048	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04414	.	stem_loop	1015	1034	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04414	.	stop_codon	1041	1041	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04414	.	polyA_site	1035	1048	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04414	.	engineered_region	1008	1048	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04414	.	engineered_region	1	911	.	+	0	ID=BBa_S04404;Name=BBa_S04404;Parent=BBa_S04409
BBa_S04414	.	CDS	227	911	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S04404
BBa_S04414	.	tag	847	880	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S04414	.	CDS	230	846	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S04414	.	engineered_tag	887	911	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S04414	.	engineered_region	1	220	.	+	0	ID=BBa_S04403;Name=BBa_S04403;Parent=BBa_S04404
BBa_S04414	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04403
BBa_S04414	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04414	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_S04403
BBa_S04414	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S04414	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S04414	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S04414	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S04414	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S04414	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S04414	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S04414	.	promoter	1057	1105	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_S04410
BBa_S04414	.	promoter	1071	1076	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_S04414	.	engineered_region	1057	1105	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_S04414	.	promoter	1094	1099	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_S04414	.	non_covalent_binding_site	1081	1097	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_S04414	.	non_covalent_binding_site	1057	1073	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_S04414	.	terminator	920	1048	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S04413
BBa_S04414	.	terminator	920	999	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04414	.	stem_loop	931	974	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04414	.	engineered_region	920	999	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04414	.	terminator	1008	1048	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04414	.	stem_loop	1015	1034	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04414	.	stop_codon	1041	1041	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04414	.	polyA_site	1035	1048	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04414	.	engineered_region	1008	1048	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04414	.	terminator	2293	2421	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S04413
BBa_S04414	.	terminator	2293	2372	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04414	.	stem_loop	2304	2347	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04414	.	engineered_region	2293	2372	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04414	.	terminator	2381	2421	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04414	.	stem_loop	2388	2407	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04414	.	stop_codon	2414	2414	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04414	.	polyA_site	2408	2421	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04414	.	engineered_region	2381	2421	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04414	.	promoter	2430	2483	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S04414	.	promoter	2449	2454	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04414	.	engineered_region	2430	2483	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04414	.	non_covalent_binding_site	2430	2448	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S04414	.	non_covalent_binding_site	2455	2473	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04414	.	promoter	2472	2477	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_S04414
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgtccagatt
agataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactc
gcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagcca
ttgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgc
taaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacag
tatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcg
ctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctac
tactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattc
ggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacg
ctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgct
actagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtc
tcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcga
tggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccac
ctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtg
gtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtg
ggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatt
tcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcat
ctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctg
gctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttt
tcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggc
gcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagaca
gctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgct
gcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcg
cccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaa
gcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagat
tgacatccctatcagtgatagagatactgagcac