LOCUS       BBa_S04432              1726 bp    DNA     linear       19-Nov-2024
DEFINITION  J70569:J70575
ACCESSION   BBa_S04432
VERSION     BBa_S04432.1  
FEATURES             Location/Qualifiers
     misc_feature    1..186
                     /label=R0010, BsaXI site removed
     CDS             193..669
                     /label=N terminal E0040
     CDS             1..669
                     /label=BBa_J70569
     misc_feature    678..681
                     /label=BsaXI partial site (only occurs when follows scar)
     misc_feature    1472..1482
                     /label=BsaXI site
     misc_feature    1717..1726
                     /label=BsaXI partial site (occurs with suffix or scar)
     misc_feature    1183..1183
                     /label=mutation from J70568: G506A (DNA), V169I (protein)
     promoter        678..847
                     /label=R0010(-naturally occurring internal BsaXI site)
     RBS             855..867
                     /label=B0034
     CDS             874..1554
                     /label=E1010
     misc_feature    1555..1579
                     /label=Junk from J04450
     misc_feature    689..691
                     /label=BsaXI potential cut site
     misc_feature    1460..1462
                     /label=BsaXI cut site
     misc_feature    1490..1492
                     /label=BsaXI cut site
     misc_feature    1707..1709
                     /label=BsaXI cut site
     misc_feature    1588..1667
                     /label=B0010
     misc_feature    1676..1716
                     /label=B0012
     misc_feature    678..1726
                     /label=BBa_J70575
ORIGIN
        1 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
       61 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      121 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagaaagag
      181 gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      241 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      301 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      361 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
      421 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
      481 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
      541 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
      601 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
      661 caataataat actagagctc cgattcatta atgcagctgg cacgacaggt ttcccgactg
      721 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      781 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      841 tcacacatac tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa
      901 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc
      961 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc
     1021 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc
     1081 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa
     1141 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtattgttac cgttacccag
     1201 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc
     1261 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt
     1321 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac
     1381 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg
     1441 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc
     1501 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga
     1561 tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1621 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1681 ctggctcacc ttcgggtggg cctttctgcg tttataggag gacagg
//