LOCUS       BBa_S04435              2289 bp    DNA     linear       25-Nov-2024
DEFINITION  I715039:K091220
ACCESSION   BBa_S04435
VERSION     BBa_S04435.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..907
                     /label=BBa_E1010
     misc_feature    209..932
                     /label=BBa_I13502
     misc_feature    1..932
                     /label=BBa_I715039
     RBS             941..955
                     /label=RBS-1\Strong
     RBS             948..952
                     /label=RBS
     misc_feature    941..955
                     /label=BBa_B0030
     RBS             941..955
                     /label=BBa_B0030
     CDS             962..2152
                     /label=tetA(C) forward
     primer_bind     962..985
                     /label=Fwd primer J31007
     primer_bind     2133..2152
                     /label=Rev primer J31007
     misc_feature    2150..2152
                     /label=stop
     CDS             962..2152
                     /label=BBa_J31007
     stem_loop       2172..2215
                     /label=stem_loop
     misc_feature    2161..2240
                     /label=BBa_B0010
     terminator      2161..2240
                     /label=BBa_B0010
     stem_loop       2256..2275
                     /label=T7 TE
     misc_feature    2282..2282
                     /label=stop
     polyA_site      2276..2289
                     /label=polya
     misc_feature    2249..2289
                     /label=BBa_B0012
     terminator      2249..2289
                     /label=BBa_B0012
     misc_feature    941..2289
                     /label=BBa_K091220
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag attaaagagg agaaatacta
      961 gatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg
     1021 cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag
     1081 catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc
     1141 acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct
     1201 acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta
     1261 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     1321 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     1381 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     1441 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     1501 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt
     1561 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
     1621 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
     1681 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc
     1741 cctcgctcaa gccttcgtca ctggccccgc caccaaacgt ttcggcgaga agcaggccat
     1801 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg
     1861 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt
     1921 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct
     1981 cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc
     2041 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt
     2101 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct aatactagag
     2161 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     2221 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     2281 gcgtttata
//