LOCUS       BBa_S04436              2219 bp    DNA     linear       22-Dec-2024
DEFINITION  K315000:K091220
ACCESSION   BBa_S04436
VERSION     BBa_S04436.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..831
                     /label=mrfp1
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             157..862
                     /label=BBa_E1010
     misc_feature    1..862
                     /label=BBa_K315000
     RBS             871..885
                     /label=RBS-1\Strong
     RBS             878..882
                     /label=RBS
     misc_feature    871..885
                     /label=BBa_B0030
     RBS             871..885
                     /label=BBa_B0030
     CDS             892..2082
                     /label=tetA(C) forward
     primer_bind     892..915
                     /label=Fwd primer J31007
     primer_bind     2063..2082
                     /label=Rev primer J31007
     misc_feature    2080..2082
                     /label=stop
     CDS             892..2082
                     /label=BBa_J31007
     stem_loop       2102..2145
                     /label=stem_loop
     misc_feature    2091..2170
                     /label=BBa_B0010
     terminator      2091..2170
                     /label=BBa_B0010
     stem_loop       2186..2205
                     /label=T7 TE
     misc_feature    2212..2212
                     /label=stop
     polyA_site      2206..2219
                     /label=polya
     misc_feature    2179..2219
                     /label=BBa_B0012
     terminator      2179..2219
                     /label=BBa_B0012
     misc_feature    871..2219
                     /label=BBa_K091220
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc
      181 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
      241 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
      301 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
      361 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
      421 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
      481 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
      541 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
      601 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
      661 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
      721 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
      781 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
      841 tgatagtgct agtgtagatc gctactagag attaaagagg agaaatacta gatgaaatct
      901 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
      961 gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt
     1021 cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc
     1081 ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc
     1141 actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc
     1201 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     1261 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     1321 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     1381 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     1441 tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     1501 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     1561 ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     1621 agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     1681 gccttcgtca ctggccccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     1741 atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
     1801 ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     1861 ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     1921 accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
     1981 agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
     2041 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct aatactagag ccaggcatca
     2101 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2161 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//