##gff-version 3
##sequence-region BBa_S04436 1 2219
BBa_S04436	.	engineered_region	871	2219	.	+	0	ID=BBa_K091220;Name=BBa_K091220
BBa_S04436	.	CDS	892	2082	.	+	0	ID=BBa_J31007;Name=BBa_J31007;Parent=BBa_K091220
BBa_S04436	.	primer_binding_site	892	915	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S04436	.	primer_binding_site	2063	2082	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S04436	.	CDS	892	2082	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S04436	.	stop_codon	2080	2082	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S04436	.	ribosome_entry_site	871	885	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K091220
BBa_S04436	.	engineered_region	871	885	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S04436	.	ribosome_entry_site	878	882	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S04436	.	ribosome_entry_site	871	885	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S04436	.	terminator	2179	2219	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K091220
BBa_S04436	.	stem_loop	2186	2205	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04436	.	polyA_site	2206	2219	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04436	.	stop_codon	2212	2212	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04436	.	engineered_region	2179	2219	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04436	.	terminator	2091	2170	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K091220
BBa_S04436	.	stem_loop	2102	2145	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04436	.	engineered_region	2091	2170	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04436	.	engineered_region	1	862	.	+	0	ID=BBa_K315000;Name=BBa_K315000
BBa_S04436	.	CDS	157	862	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K315000
BBa_S04436	.	CDS	157	831	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S04436	.	engineered_tag	838	862	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S04436	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K315000
BBa_S04436	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04436	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K315000
>BBa_S04436
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
ggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttc
gaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgc
tgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatccc
ggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttacc
gttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacg
gtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaagg
tgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaa
ccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttg
aacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactaga
gattaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgta
ggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatg
gcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccg
ccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatc
ctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccg
atggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgg
gggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggc
tgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttcc
ggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggc
agcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcgga
atcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccg
gcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattct
tctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacag
cttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcct
cggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcgg
tgcatggagccgggccacctcgacctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttata