LOCUS       BBa_S04439               989 bp    DNA     linear       22-Nov-2024
DEFINITION  J13002:K199150
ACCESSION   BBa_S04439
VERSION     BBa_S04439.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    100..100
                     /label=Transcription Start Site
     misc_feature    83..93
                     /label=Polymerase Binding Region
     misc_feature    94..105
                     /label=Initiation Region
     promoter        83..105
                     /label=BBa_R0085
     RBS             114..126
                     /label=RBS-3\Weak
     RBS             120..123
                     /label=RBS
     misc_feature    114..126
                     /label=BBa_B0032
     RBS             114..126
                     /label=BBa_B0032
     CDS             133..852
                     /label=GFP protein
     CDS             133..852
                     /label=BBa_E0040
     stem_loop       872..915
                     /label=stem_loop
     misc_feature    861..940
                     /label=BBa_B0010
     terminator      861..940
                     /label=BBa_B0010
     stem_loop       956..975
                     /label=T7 TE
     misc_feature    982..982
                     /label=stop
     polyA_site      976..989
                     /label=polya
     misc_feature    949..989
                     /label=BBa_B0012
     terminator      949..989
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag agtaatacga ctcactatag ggagatacta gagtcacaca
      121 ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      181 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      241 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      301 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
      361 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
      421 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
      481 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
      541 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
      601 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
      661 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
      721 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
      781 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
      841 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      901 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      961 accttcgggt gggcctttct gcgtttata
//