##gff-version 3
##sequence-region BBa_S04439 1 989
BBa_S04439	.	terminator	861	940	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S04439	.	engineered_region	861	940	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04439	.	stem_loop	872	915	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04439	.	promoter	83	105	.	+	0	ID=BBa_R0085;Name=BBa_R0085
BBa_S04439	.	sequence_feature	100	100	.	+	0	ID=annotation1721520;Name=Transcription Start Site;Parent=BBa_R0085
BBa_S04439	.	sequence_feature	94	105	.	+	0	ID=annotation1721522;Name=Initiation Region;Parent=BBa_R0085
BBa_S04439	.	non_covalent_binding_site	83	93	.	+	0	ID=annotation1721521;Name=Polymerase Binding Region;Parent=BBa_R0085
BBa_S04439	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S04439	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04439	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S04439	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04439	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04439	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04439	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S04439	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S04439	.	CDS	133	852	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_S04439	.	CDS	133	852	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_S04439	.	terminator	949	989	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S04439	.	stem_loop	956	975	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04439	.	polyA_site	976	989	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04439	.	stop_codon	982	982	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04439	.	engineered_region	949	989	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04439	.	ribosome_entry_site	114	126	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_S04439	.	ribosome_entry_site	114	126	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04439	.	ribosome_entry_site	120	123	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04439	.	engineered_region	114	126	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
>BBa_S04439
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gagtaatacgactcactatagggagatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcac
tggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggt
gatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtca
ctactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccat
gcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagttt
gaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaat
tggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaat
tagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtc
cttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcc
ttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata