LOCUS       BBa_S04441               837 bp    DNA     linear       27-Dec-2025
DEFINITION  pT7-RBS-RFP-pTetR-RBS
ACCESSION   BBa_S04441
VERSION     BBa_S04441.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     CDS             50..724
                     /label=mrfp1
     misc_feature    731..755
                     /label=Help:Barcodes
     CDS             50..755
                     /label=BBa_E1010
     misc_feature    1..755
                     /label=BBa_K199118
     misc_feature    764..782
                     /label=TetR 1
     misc_feature    764..817
                     /label=BBa_R0040
     promoter        783..788
                     /label=-35
     misc_feature    789..807
                     /label=TetR 2
     promoter        806..811
                     /label=-10
     promoter        764..817
                     /label=BBa_R0040
     misc_feature    830..833
                     /label=conserved
     RBS             826..837
                     /label=BBa_B0034
     promoter        764..837
                     /label=BBa_J13002
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc
       61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      481 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
      541 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
      601 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
      661 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
      721 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagtccctat cagtgataga
      781 gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga ggagaaa
//