##gff-version 3
##sequence-region BBa_S04443 1 1442
BBa_S04443	.	sequence_feature	1	1049	.	+	0	ID=BBa_J70575;Name=BBa_J70575
BBa_S04443	.	engineered_region	999	1039	.	+	0	ID=annotation2069028;Name=B0012;Parent=BBa_J70575
BBa_S04443	.	promoter	1	170	.	+	0	ID=annotation2069019;Name=R0010(-naturally occurring internal BsaXI site);Parent=BBa_J70575
BBa_S04443	.	sequence_feature	795	805	.	+	0	ID=annotation2069016;Name=BsaXI site;Parent=BBa_J70575
BBa_S04443	.	sequence_feature	1	4	.	+	0	ID=annotation2069015;Name=BsaXI partial site (only occurs when follows scar);Parent=BBa_J70575
BBa_S04443	.	sequence_feature	1030	1032	.	+	0	ID=annotation2069026;Name=BsaXI cut site;Parent=BBa_J70575
BBa_S04443	.	sequence_feature	878	902	.	+	0	ID=annotation2069022;Name=Junk from J04450;Parent=BBa_J70575
BBa_S04443	.	sequence_feature	12	14	.	+	0	ID=annotation2069023;Name=BsaXI potential cut site;Parent=BBa_J70575
BBa_S04443	.	ribosome_entry_site	178	190	.	+	0	ID=annotation2069020;Name=B0034;Parent=BBa_J70575
BBa_S04443	.	sequence_feature	783	785	.	+	0	ID=annotation2069024;Name=BsaXI cut site;Parent=BBa_J70575
BBa_S04443	.	CDS	197	877	.	+	0	ID=annotation2069021;Name=E1010;Parent=BBa_J70575
BBa_S04443	.	engineered_region	911	990	.	+	0	ID=annotation2069027;Name=B0010;Parent=BBa_J70575
BBa_S04443	.	sequence_alteration	506	506	.	+	0	ID=annotation2069018;Name=mutation from J70568: G506A (DNA), V169I (protein);Parent=BBa_J70575
BBa_S04443	.	sequence_feature	1040	1049	.	+	0	ID=annotation2069017;Name=BsaXI partial site (occurs with suffix or scar);Parent=BBa_J70575
BBa_S04443	.	sequence_feature	813	815	.	+	0	ID=annotation2069025;Name=BsaXI cut site;Parent=BBa_J70575
BBa_S04443	.	polypeptide_domain	1056	1442	.	+	0	ID=BBa_J70554;Name=BBa_J70554
>BBa_S04443
ctccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgag
ttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataa
caatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgttt
caaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggt
acccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagt
acggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatg
ggaacgtgttatgaacttcgaagacggtggtattgttaccgttacccaggactcctccctgcaagacggtgagttcatc
tacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagctt
ccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtca
ctacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaa
ctggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtg
cttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttataggaggacaggtactagatgaagaatggaatcaaagttaacttcaaaattagacacaacattgaagat
ggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccatt
acctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagc
tgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgttta