LOCUS       BBa_S04448              2149 bp    DNA     linear       23-May-2024
DEFINITION  S04446:J31007
ACCESSION   BBa_S04448
VERSION     BBa_S04448.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..907
                     /label=BBa_E1010
     misc_feature    209..932
                     /label=BBa_I13502
     misc_feature    1..932
                     /label=BBa_I715039
     misc_feature    945..948
                     /label=conserved
     RBS             941..952
                     /label=BBa_B0034
     misc_feature    1..952
                     /label=BBa_S04446
     CDS             959..2149
                     /label=tetA(C) forward
     primer_bind     959..982
                     /label=Fwd primer J31007
     primer_bind     2130..2149
                     /label=Rev primer J31007
     misc_feature    2147..2149
                     /label=stop
     CDS             959..2149
                     /label=BBa_J31007
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag aaagaggaga aatactagat
      961 gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat
     1021 aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagcat
     1081 cgccagtcac tatggcgtgc tgctagcgct atatgcgttg atgcaatttc tatgcgcacc
     1141 cgttctcgga gcactgtccg accgctttgg ccgccgccca gtcctgctcg cttcgctact
     1201 tggagccact atcgactacg cgatcatggc gaccacaccc gtcctgtgga tcctctacgc
     1261 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     1321 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     1381 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     1441 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     1501 gcaggagtcg cataagggag agcgtcgacc gatgcccttg agagccttca acccagtcag
     1561 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     1621 catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt
     1681 tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct
     1741 cgctcaagcc ttcgtcactg gccccgccac caaacgtttc ggcgagaagc aggccattat
     1801 cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg
     1861 gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca
     1921 ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc
     1981 ggctcttacc agcctaactt cgatcattgg accgctgatc gtcacggcga tttatgccgc
     2041 ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat accttgtctg
     2101 cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctaa
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