LOCUS       BBa_S04449              2005 bp    DNA     linear       22-May-2024
DEFINITION  S04447:J31007
ACCESSION   BBa_S04449
VERSION     BBa_S04449.1  
FEATURES             Location/Qualifiers
     promoter        17..22
                     /label=-35
     promoter        44..49
                     /label=-10
     promoter        1..56
                     /label=BBa_K091110
     misc_feature    69..72
                     /label=conserved
     RBS             65..76
                     /label=BBa_B0034
     CDS             83..757
                     /label=mrfp1
     misc_feature    764..788
                     /label=Help:Barcodes
     CDS             83..788
                     /label=BBa_E1010
     misc_feature    65..788
                     /label=BBa_J70390
     misc_feature    1..788
                     /label=BBa_K315008
     misc_feature    801..804
                     /label=conserved
     RBS             797..808
                     /label=BBa_B0034
     misc_feature    1..808
                     /label=BBa_S04447
     CDS             815..2005
                     /label=tetA(C) forward
     primer_bind     815..838
                     /label=Fwd primer J31007
     primer_bind     1986..2005
                     /label=Rev primer J31007
     misc_feature    2003..2005
                     /label=stop
     CDS             815..2005
                     /label=BBa_J31007
ORIGIN
        1 cgttgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggtact
       61 agagaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag agttcatgcg
      121 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg
      181 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc
      241 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt
      301 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg
      361 ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct
      421 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg
      481 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga
      541 agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta
      601 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta
      661 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca
      721 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg
      781 tagatcgcta ctagagaaag aggagaaata ctagatgaaa tctaacaatg cgctcatcgt
      841 catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc
      901 gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct
      961 agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg
     1021 ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat
     1081 catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac
     1141 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     1201 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     1261 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     1321 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     1381 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
     1441 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     1501 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg
     1561 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggccc
     1621 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct
     1681 gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct
     1741 tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga
     1801 tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat
     1861 cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt
     1921 ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc
     1981 atggagccgg gccacctcga cctaa
//