##gff-version 3 ##sequence-region BBa_S04454 1 96 BBa_S04454 . sequence_feature 63 96 . + 0 ID=BBa_K315012;Name=BBa_K315012 BBa_S04454 . sequence_alteration 78 78 . + 0 ID=annotation2071536;Name=G -> C;Parent=BBa_K315012 BBa_S04454 . sequence_alteration 82 82 . + 0 ID=annotation2071537;Name=G -> A;Parent=BBa_K315012 BBa_S04454 . sequence_feature 76 83 . + 0 ID=annotation2071523;Name=spacer region;Parent=BBa_K315012 BBa_S04454 . sequence_feature 84 96 . + 0 ID=annotation2071524;Name=inverted repeat arm;Parent=BBa_K315012 BBa_S04454 . sequence_alteration 77 77 . + 0 ID=annotation2071535;Name=T -> A;Parent=BBa_K315012 BBa_S04454 . sequence_feature 63 75 . + 0 ID=annotation2071522;Name=inverted repeat arm;Parent=BBa_K315012 BBa_S04454 . promoter 1 54 . + 0 ID=BBa_R0040;Name=BBa_R0040 BBa_S04454 . non_covalent_binding_site 1 19 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_S04454 . promoter 43 48 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_S04454 . non_covalent_binding_site 26 44 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_S04454 . promoter 20 25 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_S04454 . engineered_region 1 54 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 >BBa_S04454 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataaact atactatacgaagttat