LOCUS       BBa_S04461              2045 bp    DNA     linear       27-Jun-2024
DEFINITION  S04445:S03710
ACCESSION   BBa_S04461
VERSION     BBa_S04461.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..96
                     /label=BBa_J61046
     misc_feature    1..96
                     /label=BBa_S04437
     misc_feature    109..112
                     /label=conserved
     RBS             105..116
                     /label=BBa_B0034
     CDS             123..797
                     /label=mrfp1
     misc_feature    804..828
                     /label=Help:Barcodes
     CDS             123..828
                     /label=BBa_E1010
     misc_feature    105..828
                     /label=BBa_K093005
     misc_feature    1..828
                     /label=BBa_S04445
     misc_feature    841..844
                     /label=conserved
     RBS             837..848
                     /label=BBa_B0034
     CDS             855..2045
                     /label=tetA(C) forward
     primer_bind     855..878
                     /label=Fwd primer J31007
     primer_bind     2026..2045
                     /label=Rev primer J31007
     misc_feature    2043..2045
                     /label=stop
     CDS             855..2045
                     /label=BBa_J31007
     misc_feature    837..2045
                     /label=BBa_S03710
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agataacttc gtataatgta tgctatacga agttattact agagaaagag gagaaatact
      121 agatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag
      181 gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag
      241 gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca
      301 tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc
      361 cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg
      421 aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct
      481 acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa
      541 ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg
      601 aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca
      661 cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg
      721 acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc
      781 gtcactccac cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagaaag
      841 aggagaaata ctagatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc
      901 tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg
      961 tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc
     1021 aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc
     1081 tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc
     1141 tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     1201 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     1261 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     1321 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     1381 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag
     1441 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     1501 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
     1561 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
     1621 gaatcttgca cgccctcgct caagccttcg tcactggccc cgccaccaaa cgtttcggcg
     1681 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
     1741 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
     1801 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
     1861 ttcaaggatc gctcgcggct cttaccagcc taacttcgat cattggaccg ctgatcgtca
     1921 cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg
     1981 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2041 cctaa
//