##gff-version 3
##sequence-region BBa_S04461 1 2045
BBa_S04461	.	engineered_region	837	2045	.	+	0	ID=BBa_S03710;Name=BBa_S03710
BBa_S04461	.	CDS	855	2045	.	+	0	ID=BBa_J31007;Name=BBa_J31007;Parent=BBa_S03710
BBa_S04461	.	primer_binding_site	855	878	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_S04461	.	primer_binding_site	2026	2045	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_S04461	.	CDS	855	2045	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_S04461	.	stop_codon	2043	2045	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_S04461	.	ribosome_entry_site	837	848	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03710
BBa_S04461	.	conserved_region	841	844	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04461	.	engineered_region	1	828	.	+	0	ID=BBa_S04445;Name=BBa_S04445
BBa_S04461	.	engineered_region	105	828	.	+	0	ID=BBa_K093005;Name=BBa_K093005;Parent=BBa_S04445
BBa_S04461	.	CDS	123	828	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K093005
BBa_S04461	.	CDS	123	797	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S04461	.	engineered_tag	804	828	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S04461	.	ribosome_entry_site	105	116	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K093005
BBa_S04461	.	conserved_region	109	112	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04461	.	engineered_region	1	96	.	+	0	ID=BBa_S04437;Name=BBa_S04437;Parent=BBa_S04445
BBa_S04461	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S04437
BBa_S04461	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S04461	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04461	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04461	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04461	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S04461	.	sequence_feature	63	96	.	+	0	ID=BBa_J61046;Name=BBa_J61046;Parent=BBa_S04437
>BBa_S04461
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataatgt
atgctatacgaagttattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatg
cgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacg
aaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagtt
ccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttc
aaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagt
tcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttggga
agcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggt
ggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgaca
tcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccac
cggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgaaatctaacaat
gcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctct
tgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttct
atgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccact
atcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcg
ccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgag
cgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattc
cttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtc
gaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttat
gactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgc
tggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggcc
ccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggc
gttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcag
gccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaactt
cgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtagg
cgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa