BBa_S04461
type: ComponentDefinition
persistent identity: BBa S04461
display id: BBa_S04461
version: 1
was generated by: iGEM to SBOL conversion
description: S04445:S03710
created: 2010-07-13T11:00:00Z
modified: 2015-05-08T01:14:40Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 7370
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Brianna Pearson
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S03710
component: BBa_S04445
sequence annotation: BBa_S04445
sequence annotation: BBa_S03710
sequence: https://synbiohub.org/public/igem/BBa_S04461_sequence/1

BBa_S03710
type: Component
persistent identity: component2263578
display id: component2263578
version: 1
top level: BBa_S04461
definition: BBa_S03710
access: public

BBa_S04445
type: Component
persistent identity: component2263570
display id: component2263570
version: 1
top level: BBa_S04461
definition: BBa_S04445
access: public

BBa_S04445
type: SequenceAnnotation
persistent identity: annotation2263570
display id: annotation2263570
version: 1
top level: BBa_S04461
location: https://synbiohub.org/public/igem/BBa_S04461/annotation2263570/range2263570/1
component: BBa_S04445

https://synbiohub.org/public/igem/BBa_S04461/annotation2263570/range2263570/1
type: Range
persistent identity: range2263570
display id: range2263570
version: 1
top level: BBa_S04461
direction: direction/reverse
start: 1
end: 828
orientation: inline

BBa_S03710
type: SequenceAnnotation
persistent identity: annotation2263578
display id: annotation2263578
version: 1
top level: BBa_S04461
location: https://synbiohub.org/public/igem/BBa_S04461/annotation2263578/range2263578/1
component: BBa_S03710

https://synbiohub.org/public/igem/BBa_S04461/annotation2263578/range2263578/1
type: Range
persistent identity: range2263578
display id: range2263578
version: 1
top level: BBa_S04461
direction: direction/reverse
start: 837
end: 2045
orientation: inline

BBa_S04445
type: ComponentDefinition
persistent identity: BBa S04445
display id: BBa_S04445
version: 1
was generated by: iGEM to SBOL conversion
description: S04437:K093005
created: 2010-06-24T11:00:00Z
modified: 2015-05-08T01:14:40Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 7370
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Brianna Pearson
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K093005
component: BBa_S04437
sequence annotation: BBa_S04437
sequence annotation: BBa_K093005
sequence: https://synbiohub.org/public/igem/BBa_S04445_sequence/1

BBa_K093005
type: Component
persistent identity: component2250187
display id: component2250187
version: 1
top level: BBa_S04445
definition: RBS-RFP
access: public

BBa_S04437
type: Component
persistent identity: component2250181
display id: component2250181
version: 1
top level: BBa_S04445
definition: BBa_S04437
access: public

BBa_S04437
type: SequenceAnnotation
persistent identity: annotation2250181
display id: annotation2250181
version: 1
top level: BBa_S04445
location: https://synbiohub.org/public/igem/BBa_S04445/annotation2250181/range2250181/1
component: BBa_S04437

https://synbiohub.org/public/igem/BBa_S04445/annotation2250181/range2250181/1
type: Range
persistent identity: range2250181
display id: range2250181
version: 1
top level: BBa_S04445
direction: direction/reverse
start: 1
end: 96
orientation: inline

BBa_K093005
type: SequenceAnnotation
persistent identity: annotation2250187
display id: annotation2250187
version: 1
top level: BBa_S04445
location: https://synbiohub.org/public/igem/BBa_S04445/annotation2250187/range2250187/1
component: BBa_K093005

https://synbiohub.org/public/igem/BBa_S04445/annotation2250187/range2250187/1
type: Range
persistent identity: range2250187
display id: range2250187
version: 1
top level: BBa_S04445
direction: direction/reverse
start: 105
end: 828
orientation: inline

BBa_S03710
type: ComponentDefinition
persistent identity: BBa S03710
display id: BBa_S03710
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-Tet
created: 2007-06-18T11:00:00Z
modified: 2015-05-08T01:14:29Z
owned by: james
owned by: myers
discontinued: false
dominant: true
experience: experience/None
group u list: _120_
m user id: 0
owner id: 1803
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Tom Crowley
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J31007
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J31007
sequence: https://synbiohub.org/public/igem/BBa_S03710_sequence/1

BBa_J31007
type: Component
persistent identity: component1934936
display id: component1934936
version: 1
top level: BBa_S03710
definition: tetA(C)f
access: public

BBa_B0034
type: Component
persistent identity: component1934931
display id: component1934931
version: 1
top level: BBa_S03710
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1934931
display id: annotation1934931
version: 1
top level: BBa_S03710
location: https://synbiohub.org/public/igem/BBa_S03710/annotation1934931/range1934931/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03710/annotation1934931/range1934931/1
type: Range
persistent identity: range1934931
display id: range1934931
version: 1
top level: BBa_S03710
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_J31007
type: SequenceAnnotation
persistent identity: annotation1934936
display id: annotation1934936
version: 1
top level: BBa_S03710
location: https://synbiohub.org/public/igem/BBa_S03710/annotation1934936/range1934936/1
component: BBa_J31007

https://synbiohub.org/public/igem/BBa_S03710/annotation1934936/range1934936/1
type: Range
persistent identity: range1934936
display id: range1934936
version: 1
top level: BBa_S03710
direction: direction/reverse
start: 19
end: 1209
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_S04437
type: ComponentDefinition
persistent identity: BBa S04437
display id: BBa_S04437
version: 1
was generated by: iGEM to SBOL conversion
description: R0040:J61046
created: 2010-06-13T11:00:00Z
modified: 2015-05-08T01:14:40Z
owned by: james
owned by: myers
discontinued: false
dominant: true
group u list: _61_
m user id: 0
owner id: 201
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Malcolm Campbell
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J61046
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_J61046
sequence: https://synbiohub.org/public/igem/BBa_S04437_sequence/1

BBa_J61046
type: Component
persistent identity: component2070860
display id: component2070860
version: 1
top level: BBa_S04437
definition: lox
access: public

BBa_R0040
type: Component
persistent identity: component2070855
display id: component2070855
version: 1
top level: BBa_S04437
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2070855
display id: annotation2070855
version: 1
top level: BBa_S04437
location: https://synbiohub.org/public/igem/BBa_S04437/annotation2070855/range2070855/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_S04437/annotation2070855/range2070855/1
type: Range
persistent identity: range2070855
display id: range2070855
version: 1
top level: BBa_S04437
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_J61046
type: SequenceAnnotation
persistent identity: annotation2070860
display id: annotation2070860
version: 1
top level: BBa_S04437
location: https://synbiohub.org/public/igem/BBa_S04437/annotation2070860/range2070860/1
component: BBa_J61046

https://synbiohub.org/public/igem/BBa_S04437/annotation2070860/range2070860/1
type: Range
persistent identity: range2070860
display id: range2070860
version: 1
top level: BBa_S04437
direction: direction/reverse
start: 63
end: 96
orientation: inline

RBS-RFP
type: ComponentDefinition
persistent identity: BBa K093005
display id: BBa_K093005
version: 1
was generated by: iGEM to SBOL conversion
description: RFP with RBS
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: registry plasmids
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RFP, BBa_E1010, with Elowitz RBS, BBa_B0034.
discontinued: false
dominant: false
experience: experience/Works
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The part was needed for downstream applications. There can be no expression of a reporter without a ribosome binding site.
star: true
creator: Kathy Lam, Danielle Nash
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K093005_sequence/1

BBa_B0034
type: Component
persistent identity: component2244022
display id: component2244022
version: 1
top level: RBS-RFP
definition: BBa_B0034
access: public

BBa_E1010
type: Component
persistent identity: component2244025
display id: component2244025
version: 1
top level: RBS-RFP
definition: mRFP1
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2244022
display id: annotation2244022
version: 1
top level: RBS-RFP
location: https://synbiohub.org/public/igem/BBa_K093005/annotation2244022/range2244022/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K093005/annotation2244022/range2244022/1
type: Range
persistent identity: range2244022
display id: range2244022
version: 1
top level: RBS-RFP
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2244025
display id: annotation2244025
version: 1
top level: RBS-RFP
location: https://synbiohub.org/public/igem/BBa_K093005/annotation2244025/range2244025/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K093005/annotation2244025/range2244025/1
type: Range
persistent identity: range2244025
display id: range2244025
version: 1
top level: RBS-RFP
direction: direction/reverse
start: 19
end: 724
orientation: inline

tetA(C)f
type: ComponentDefinition
persistent identity: BBa J31007
display id: BBa_J31007
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This part is the coding region of the TetR gene. It requires a promoter and RBS for the cell to be able to express tetracyline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plamsid.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetA(C) forward
sequence annotation: Fwd primer J31007
sequence annotation: Rev primer J31007
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J31007_sequence/1

tetA(C) forward
type: SequenceAnnotation
persistent identity: annotation1885000
display id: annotation1885000
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
type: Range
persistent identity: range1885000
display id: range1885000
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 1191
orientation: inline

Fwd primer J31007
type: SequenceAnnotation
persistent identity: annotation1910571
display id: annotation1910571
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
type: Range
persistent identity: range1910571
display id: range1910571
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 24
orientation: inline

Rev primer J31007
type: SequenceAnnotation
persistent identity: annotation1910572
display id: annotation1910572
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
type: Range
persistent identity: range1910572
display id: range1910572
version: 1
top level: tetA(C)f
direction: direction/reverse
start: 1172
end: 1191
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1910573
display id: annotation1910573
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
type: Range
persistent identity: range1910573
display id: range1910573
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1189
end: 1191
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

lox
type: ComponentDefinition
persistent identity: BBa J61046
display id: BBa_J61046
version: 1
was generated by: iGEM to SBOL conversion
description: [Lox] site for recombination
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: bob
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: bob
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: bob
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J61046_sequence/1

https://synbiohub.org/public/igem/BBa_S04461_sequence/1
type: Sequence
persistent identity: BBa S04461 sequence
display id: BBa_S04461_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataatgtatgctatacgaagttattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04445_sequence/1
type: Sequence
persistent identity: BBa S04445 sequence
display id: BBa_S04445_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataatgtatgctatacgaagttattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03710_sequence/1
type: Sequence
persistent identity: BBa S03710 sequence
display id: BBa_S03710_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04437_sequence/1
type: Sequence
persistent identity: BBa S04437 sequence
display id: BBa_S04437_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataatgtatgctatacgaagttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K093005_sequence/1
type: Sequence
persistent identity: BBa K093005 sequence
display id: BBa_K093005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31007_sequence/1
type: Sequence
persistent identity: BBa J31007 sequence
display id: BBa_J31007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61046_sequence/1
type: Sequence
persistent identity: BBa J61046 sequence
display id: BBa_J61046_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttcgtataatgtatgctatacgaagttat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z