LOCUS       BBa_S04469              1752 bp    DNA     linear       26-Jun-2024
DEFINITION  pT7-RBS-GFP-TT-pT7-RBS-RFP-pTetR-RBS
ACCESSION   BBa_S04469
VERSION     BBa_S04469.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     RBS             32..44
                     /label=RBS-3\Weak
     RBS             38..41
                     /label=RBS
     misc_feature    32..44
                     /label=BBa_B0032
     RBS             32..44
                     /label=BBa_B0032
     CDS             51..770
                     /label=GFP protein
     CDS             51..770
                     /label=BBa_E0040
     stem_loop       790..833
                     /label=stem_loop
     misc_feature    779..858
                     /label=BBa_B0010
     terminator      779..858
                     /label=BBa_B0010
     stem_loop       874..893
                     /label=T7 TE
     misc_feature    900..900
                     /label=stop
     polyA_site      894..907
                     /label=polya
     misc_feature    867..907
                     /label=BBa_B0012
     terminator      867..907
                     /label=BBa_B0012
     misc_feature    1..907
                     /label=BBa_K199150
     misc_feature    933..933
                     /label=Transcription Start Site
     misc_feature    916..926
                     /label=Polymerase Binding Region
     misc_feature    927..938
                     /label=Initiation Region
     promoter        916..938
                     /label=BBa_R0085
     misc_feature    951..954
                     /label=conserved
     RBS             947..958
                     /label=BBa_B0034
     CDS             965..1639
                     /label=mrfp1
     misc_feature    1646..1670
                     /label=Help:Barcodes
     CDS             965..1670
                     /label=BBa_E1010
     misc_feature    916..1670
                     /label=BBa_K199118
     misc_feature    1679..1697
                     /label=TetR 1
     misc_feature    1679..1732
                     /label=BBa_R0040
     promoter        1698..1703
                     /label=-35
     misc_feature    1704..1722
                     /label=TetR 2
     promoter        1721..1726
                     /label=-10
     promoter        1679..1732
                     /label=BBa_R0040
     misc_feature    1745..1748
                     /label=conserved
     RBS             1741..1752
                     /label=BBa_B0034
     promoter        1679..1752
                     /label=BBa_J13002
     misc_feature    916..1752
                     /label=BBa_S04441
ORIGIN
        1 taatacgact cactataggg agatactaga gtcacacagg aaagtactag atgcgtaaag
       61 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      121 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
      181 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
      241 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
      301 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
      361 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
      421 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
      481 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
      541 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
      601 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
      661 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
      721 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc
      781 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      841 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      901 gtttatatac tagagtaata cgactcacta tagggagata ctagagaaag aggagaaata
      961 ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga
     1021 aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga
     1081 aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga
     1141 catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat
     1201 cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt
     1261 cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat
     1321 ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa
     1381 aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg
     1441 tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac
     1501 cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact
     1561 ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg
     1621 tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagtc
     1681 cctatcagtg atagagattg acatccctat cagtgataga gatactgagc actactagag
     1741 aaagaggaga aa
//