LOCUS       BBa_S04470              3466 bp    DNA     linear       01-Jun-2024
DEFINITION  K091225:S04446
ACCESSION   BBa_S04470
VERSION     BBa_S04470.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    1246..1284
                     /label=LVA
     CDS             157..1284
                     /label=lacI-LVA
     misc_feature    157..1284
                     /label=BBa_C0012
     misc_feature    1285..1309
                     /label=Help:Barcodes
     CDS             157..1284
                     /label=BBa_C0012
     misc_feature    139..1309
                     /label=BBa_S0100
     promoter        1..1309
                     /label=BBa_K091225
     CDS             1318..1405
                     /label=end of LacI coding region (inactive)
     misc_feature    1318..1517
                     /label=BBa_R0010
     misc_feature    1406..1443
                     /label=CAP binding site
     promoter        1454..1459
                     /label=-35
     promoter        1478..1483
                     /label=-10
     misc_feature    1483..1517
                     /label=LacI binding site
     misc_feature    1490..1490
                     /label=start
     promoter        1318..1517
                     /label=BBa_R0010
     misc_feature    1530..1533
                     /label=conserved
     RBS             1526..1537
                     /label=BBa_B0034
     CDS             1544..2218
                     /label=mrfp1
     misc_feature    2225..2249
                     /label=Help:Barcodes
     CDS             1544..2224
                     /label=BBa_E1010
     misc_feature    1526..2249
                     /label=BBa_I13502
     misc_feature    1318..2249
                     /label=BBa_I715039
     misc_feature    2262..2265
                     /label=conserved
     RBS             2258..2269
                     /label=BBa_B0034
     misc_feature    1318..2269
                     /label=BBa_S04446
     CDS             2276..3466
                     /label=tetA(C) forward
     primer_bind     2276..2299
                     /label=Fwd primer J31007
     primer_bind     3447..3466
                     /label=Rev primer J31007
     misc_feature    3464..3466
                     /label=stop
     CDS             2276..3466
                     /label=BBa_J31007
     misc_feature    1318..3466
                     /label=BBa_S04448
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg
      181 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac
      241 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg
      301 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc
      361 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct
      421 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa
      481 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac
      541 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg
      601 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac
      661 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta
      721 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc
      781 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt
      841 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc
      901 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt
      961 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg
     1021 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg
     1081 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa
     1141 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1201 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa
     1261 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcaa
     1321 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1381 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     1441 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     1501 gataacaatt tcacacatac tagagaaaga ggagaaatac tagatggctt cctccgaaga
     1561 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga
     1621 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact
     1681 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca
     1741 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc
     1801 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac
     1861 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg
     1921 taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc
     1981 caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa
     2041 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc
     2101 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga
     2161 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta
     2221 ataacgctga tagtgctagt gtagatcgct actagagaaa gaggagaaat actagatgaa
     2281 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
     2341 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
     2401 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt
     2461 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg
     2521 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg
     2581 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     2641 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     2701 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     2761 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     2821 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
     2881 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     2941 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
     3001 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
     3061 tcaagccttc gtcactggcc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
     3121 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
     3181 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     3241 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc
     3301 tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt atgccgcctc
     3361 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct
     3421 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctaa
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