LOCUS       BBa_S04471              3322 bp    DNA     linear       01-Jun-2024
DEFINITION  K091225:S04447
ACCESSION   BBa_S04471
VERSION     BBa_S04471.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    1246..1284
                     /label=LVA
     CDS             157..1284
                     /label=lacI-LVA
     misc_feature    157..1284
                     /label=BBa_C0012
     misc_feature    1285..1309
                     /label=Help:Barcodes
     CDS             157..1284
                     /label=BBa_C0012
     misc_feature    139..1309
                     /label=BBa_S0100
     promoter        1..1309
                     /label=BBa_K091225
     promoter        1334..1339
                     /label=-35
     promoter        1361..1366
                     /label=-10
     promoter        1318..1373
                     /label=BBa_K091110
     misc_feature    1386..1389
                     /label=conserved
     RBS             1382..1393
                     /label=BBa_B0034
     CDS             1400..2074
                     /label=mrfp1
     misc_feature    2081..2105
                     /label=Help:Barcodes
     CDS             1400..2105
                     /label=BBa_E1010
     misc_feature    1382..2105
                     /label=BBa_J70390
     misc_feature    1318..2105
                     /label=BBa_K315008
     misc_feature    2118..2121
                     /label=conserved
     RBS             2114..2125
                     /label=BBa_B0034
     CDS             2132..3322
                     /label=tetA(C) forward
     primer_bind     2132..2155
                     /label=Fwd primer J31007
     primer_bind     3303..3322
                     /label=Rev primer J31007
     misc_feature    3320..3322
                     /label=stop
     CDS             2132..3322
                     /label=BBa_J31007
     misc_feature    2114..3322
                     /label=BBa_J85100
     misc_feature    1318..3322
                     /label=BBa_K315009
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg
      181 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac
      241 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg
      301 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc
      361 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct
      421 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa
      481 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac
      541 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg
      601 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac
      661 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta
      721 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc
      781 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt
      841 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc
      901 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt
      961 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg
     1021 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg
     1081 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa
     1141 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1201 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa
     1261 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcgt
     1321 tgacaccatc gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggtactaga
     1381 gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt
     1441 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
     1501 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
     1561 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
     1621 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
     1681 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
     1741 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
     1801 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga
     1861 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
     1921 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa
     1981 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
     2041 cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag
     2101 atcgctacta gagaaagagg agaaatacta gatgaaatct aacaatgcgc tcatcgtcat
     2161 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
     2221 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
     2281 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
     2341 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
     2401 ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg
     2461 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     2521 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     2581 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     2641 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     2701 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     2761 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     2821 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     2881 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggccccgc
     2941 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     3001 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     3061 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     3121 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     3181 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     3241 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     3301 gagccgggcc acctcgacct aa
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