LOCUS       BBa_S04472              2625 bp    DNA     linear       23-Dec-2024
DEFINITION  S04419:K199175
ACCESSION   BBa_S04472
VERSION     BBa_S04472.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..693
                     /label=mrfp1
     misc_feature    700..724
                     /label=Help:Barcodes
     CDS             19..724
                     /label=BBa_E1010
     misc_feature    1..724
                     /label=BBa_S04419
     RBS             733..747
                     /label=RBS-1\Strong
     RBS             740..744
                     /label=RBS
     misc_feature    733..747
                     /label=BBa_B0030
     RBS             733..747
                     /label=BBa_B0030
     CDS             754..1473
                     /label=GFP protein
     CDS             754..1473
                     /label=BBa_E0040
     misc_feature    733..1473
                     /label=BBa_E5500
     CDS             1482..1569
                     /label=end of LacI coding region (inactive)
     misc_feature    1482..1681
                     /label=BBa_R0010
     misc_feature    1570..1607
                     /label=CAP binding site
     promoter        1618..1623
                     /label=-35
     promoter        1642..1647
                     /label=-10
     misc_feature    1647..1681
                     /label=LacI binding site
     misc_feature    1654..1654
                     /label=start
     promoter        1482..1681
                     /label=BBa_R0010
     misc_feature    1694..1697
                     /label=conserved
     RBS             1690..1701
                     /label=BBa_B0034
     misc_feature    1708..1709
                     /label=prefix
     misc_feature    1881..1881
                     /label=T
     misc_feature    2199..2199
                     /label=A
     CDS             1708..2457
                     /label=luxR
     misc_feature    1708..2463
                     /label=BBa_C0062
     misc_feature    2464..2488
                     /label=Help:Barcodes
     CDS             1708..2463
                     /label=BBa_C0062
     stem_loop       2508..2551
                     /label=stem_loop
     misc_feature    2497..2576
                     /label=BBa_B0010
     terminator      2497..2576
                     /label=BBa_B0010
     stem_loop       2592..2611
                     /label=T7 TE
     misc_feature    2618..2618
                     /label=stop
     polyA_site      2612..2625
                     /label=polya
     misc_feature    2585..2625
                     /label=BBa_B0012
     terminator      2585..2625
                     /label=BBa_B0012
     misc_feature    1690..2625
                     /label=BBa_I0462
     misc_feature    1482..2625
                     /label=BBa_K109702
     misc_feature    733..2625
                     /label=BBa_K199175
ORIGIN
        1 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc
       61 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      121 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      181 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
      241 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
      301 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
      361 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
      421 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
      481 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
      541 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
      601 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
      661 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga
      721 tcgctactag agattaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact
      781 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      841 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      901 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa
      961 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
     1021 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
     1081 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
     1141 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
     1201 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
     1261 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
     1321 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
     1381 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
     1441 acacatggca tggatgaact atacaaataa taatactaga gcaatacgca aaccgcctct
     1501 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     1561 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     1621 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     1681 atactagaga aagaggagaa atactagatg aaaaacataa atgccgacga cacatacaga
     1741 ataattaata aaattaaagc ttgtagaagc aataatgata ttaatcaatg cttatctgat
     1801 atgactaaaa tggtacattg tgaatattat ttactcgcga tcatttatcc tcattctatg
     1861 gttaaatctg atatttcaat cctagataat taccctaaaa aatggaggca atattatgat
     1921 gacgctaatt taataaaata tgatcctata gtagattatt ctaactccaa tcattcacca
     1981 attaattgga atatatttga aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa
     2041 gaagcgaaaa catcaggtct tatcactggg tttagtttcc ctattcatac ggctaacaat
     2101 ggcttcggaa tgcttagttt tgcacattca gaaaaagaca actatataga tagtttattt
     2161 ttacatgcgt gtatgaacat accattaatt gttccttctc tagttgataa ttatcgaaaa
     2221 ataaatatag caaataataa atcaaacaac gatttaacca aaagagaaaa agaatgttta
     2281 gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa aaatattagg ttgcagtgag
     2341 cgtactgtca ctttccattt aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc
     2401 caaagtattt ctaaagcaat tttaacagga gcaattgatt gcccatactt taaaaattaa
     2461 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg
     2521 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     2581 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//